Job ID = 10721710 sra ファイルのダウンロード中... Completed: 851698K bytes transferred in 68 seconds (101333K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 24487011 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766297/SRR5482925.sra Written 24487011 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766297/SRR5482925.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:58 24487011 reads; of these: 24487011 (100.00%) were unpaired; of these: 1096356 (4.48%) aligned 0 times 16190353 (66.12%) aligned exactly 1 time 7200302 (29.40%) aligned >1 times 95.52% overall alignment rate Time searching: 00:04:58 Overall time: 00:04:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15797721 / 23390655 = 0.6754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 04 Jun 2018 03:26:43: # Command line: callpeak -t SRX2766297.bam -f BAM -g 12100000 -n SRX2766297.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2766297.10 # format = BAM # ChIP-seq file = ['SRX2766297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:26:43: # Command line: callpeak -t SRX2766297.bam -f BAM -g 12100000 -n SRX2766297.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2766297.05 # format = BAM # ChIP-seq file = ['SRX2766297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:26:43: # Command line: callpeak -t SRX2766297.bam -f BAM -g 12100000 -n SRX2766297.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2766297.20 # format = BAM # ChIP-seq file = ['SRX2766297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:26:43: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:26:43: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:26:43: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:26:43: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:26:43: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:26:43: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:26:50: 1000000 INFO @ Mon, 04 Jun 2018 03:26:50: 1000000 INFO @ Mon, 04 Jun 2018 03:26:50: 1000000 INFO @ Mon, 04 Jun 2018 03:26:57: 2000000 INFO @ Mon, 04 Jun 2018 03:26:57: 2000000 INFO @ Mon, 04 Jun 2018 03:26:58: 2000000 INFO @ Mon, 04 Jun 2018 03:27:04: 3000000 INFO @ Mon, 04 Jun 2018 03:27:04: 3000000 INFO @ Mon, 04 Jun 2018 03:27:05: 3000000 INFO @ Mon, 04 Jun 2018 03:27:11: 4000000 INFO @ Mon, 04 Jun 2018 03:27:11: 4000000 INFO @ Mon, 04 Jun 2018 03:27:12: 4000000 INFO @ Mon, 04 Jun 2018 03:27:18: 5000000 INFO @ Mon, 04 Jun 2018 03:27:18: 5000000 INFO @ Mon, 04 Jun 2018 03:27:20: 5000000 INFO @ Mon, 04 Jun 2018 03:27:25: 6000000 INFO @ Mon, 04 Jun 2018 03:27:25: 6000000 INFO @ Mon, 04 Jun 2018 03:27:27: 6000000 INFO @ Mon, 04 Jun 2018 03:27:32: 7000000 INFO @ Mon, 04 Jun 2018 03:27:32: 7000000 INFO @ Mon, 04 Jun 2018 03:27:34: 7000000 INFO @ Mon, 04 Jun 2018 03:27:36: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:27:36: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:27:36: #1 total tags in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:36: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:27:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:27:36: #1 tags after filtering in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:36: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:27:36: #1 finished! INFO @ Mon, 04 Jun 2018 03:27:36: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:27:36: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Jun 2018 03:27:37: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:27:37: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:27:37: #1 total tags in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:37: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:27:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:27:37: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:27:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:27:37: Process for pairing-model is terminated! INFO @ Mon, 04 Jun 2018 03:27:37: #1 tags after filtering in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:37: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:27:37: #1 finished! INFO @ Mon, 04 Jun 2018 03:27:37: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:27:37: #2 looking for paired plus/minus strand peaks... cat: SRX2766297.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766297.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 04 Jun 2018 03:27:37: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:27:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:27:37: Process for pairing-model is terminated! cat: SRX2766297.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766297.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 04 Jun 2018 03:27:38: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:27:38: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:27:38: #1 total tags in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:38: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:27:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:27:38: #1 tags after filtering in treatment: 7592934 INFO @ Mon, 04 Jun 2018 03:27:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:27:38: #1 finished! INFO @ Mon, 04 Jun 2018 03:27:38: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:27:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Jun 2018 03:27:39: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:27:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:27:39: Process for pairing-model is terminated! cat: SRX2766297.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766297.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766297.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。