Job ID = 10609001 sra ファイルのダウンロード中... Completed: 229040K bytes transferred in 22 seconds (81839K bits/sec), in 1 file. Completed: 229297K bytes transferred in 17 seconds (105244K bits/sec), in 1 file. Completed: 230332K bytes transferred in 21 seconds (85851K bits/sec), in 1 file. Completed: 229404K bytes transferred in 15 seconds (120850K bits/sec), in 1 file. Completed: 228813K bytes transferred in 17 seconds (107748K bits/sec), in 1 file. Completed: 231133K bytes transferred in 25 seconds (73399K bits/sec), in 1 file. Completed: 92774K bytes transferred in 8 seconds (86679K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 1564806 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457349.sra Written 1564806 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457344.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457343.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457347.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457348.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457345.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745403/SRR5457346.sra Written 4000000 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:05 25564806 reads; of these: 25564806 (100.00%) were unpaired; of these: 7768411 (30.39%) aligned 0 times 11577167 (45.29%) aligned exactly 1 time 6219228 (24.33%) aligned >1 times 69.61% overall alignment rate Time searching: 00:06:05 Overall time: 00:06:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 12405450 / 17796395 = 0.6971 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:12:32: # Command line: callpeak -t SRX2745403.bam -f BAM -g 12100000 -n SRX2745403.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2745403.10 # format = BAM # ChIP-seq file = ['SRX2745403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:12:32: #1 read tag files... INFO @ Thu, 03 May 2018 23:12:32: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:12:32: # Command line: callpeak -t SRX2745403.bam -f BAM -g 12100000 -n SRX2745403.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2745403.05 # format = BAM # ChIP-seq file = ['SRX2745403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:12:32: #1 read tag files... INFO @ Thu, 03 May 2018 23:12:32: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:12:32: # Command line: callpeak -t SRX2745403.bam -f BAM -g 12100000 -n SRX2745403.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2745403.20 # format = BAM # ChIP-seq file = ['SRX2745403.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:12:32: #1 read tag files... INFO @ Thu, 03 May 2018 23:12:32: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:12:40: 1000000 INFO @ Thu, 03 May 2018 23:12:40: 1000000 INFO @ Thu, 03 May 2018 23:12:40: 1000000 INFO @ Thu, 03 May 2018 23:12:47: 2000000 INFO @ Thu, 03 May 2018 23:12:47: 2000000 INFO @ Thu, 03 May 2018 23:12:47: 2000000 INFO @ Thu, 03 May 2018 23:12:55: 3000000 INFO @ Thu, 03 May 2018 23:12:55: 3000000 INFO @ Thu, 03 May 2018 23:12:55: 3000000 INFO @ Thu, 03 May 2018 23:13:03: 4000000 INFO @ Thu, 03 May 2018 23:13:03: 4000000 INFO @ Thu, 03 May 2018 23:13:03: 4000000 INFO @ Thu, 03 May 2018 23:13:10: 5000000 INFO @ Thu, 03 May 2018 23:13:10: 5000000 INFO @ Thu, 03 May 2018 23:13:10: 5000000 INFO @ Thu, 03 May 2018 23:13:13: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:13:13: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:13:13: #1 total tags in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:13:13: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:13:13: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:13:13: #1 total tags in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:13:13: #1 tags after filtering in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:13:13: #1 finished! INFO @ Thu, 03 May 2018 23:13:13: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:13:13: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:13:13: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:13:13: #1 total tags in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:13:13: #1 tags after filtering in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:13:13: #1 finished! INFO @ Thu, 03 May 2018 23:13:13: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:13:13: #1 tags after filtering in treatment: 5390945 INFO @ Thu, 03 May 2018 23:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:13:13: #1 finished! INFO @ Thu, 03 May 2018 23:13:13: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:13:14: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:13:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:13:14: Process for pairing-model is terminated! cat: SRX2745403.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745403.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:13:14: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:13:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:13:14: Process for pairing-model is terminated! cat: SRX2745403.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745403.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:13:14: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:13:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:13:14: Process for pairing-model is terminated! cat: SRX2745403.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745403.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745403.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。