Job ID = 10608995 sra ファイルのダウンロード中... Completed: 230184K bytes transferred in 30 seconds (62235K bits/sec), in 1 file. Completed: 4472K bytes transferred in 2 seconds (13002K bits/sec), in 1 file. Completed: 230881K bytes transferred in 29 seconds (63225K bits/sec), in 1 file. Completed: 230723K bytes transferred in 13 seconds (137839K bits/sec), in 1 file. Completed: 228430K bytes transferred in 12 seconds (145209K bits/sec), in 1 file. Completed: 231583K bytes transferred in 14 seconds (126994K bits/sec), in 1 file. Completed: 233456K bytes transferred in 22 seconds (85369K bits/sec), in 1 file. Completed: 227395K bytes transferred in 19 seconds (97437K bits/sec), in 1 file. Completed: 228796K bytes transferred in 19 seconds (97348K bits/sec), in 1 file. Completed: 234414K bytes transferred in 14 seconds (131640K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 73304 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457329.sra Written 73304 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457330.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457334.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457331.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457336.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457332.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457337.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457335.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457333.sra Written 4000000 spots total Written 4000000 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745397/SRR5457328.sra Written 4000000 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 36073304 reads; of these: 36073304 (100.00%) were unpaired; of these: 10961778 (30.39%) aligned 0 times 16441063 (45.58%) aligned exactly 1 time 8670463 (24.04%) aligned >1 times 69.61% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16306943 / 25111526 = 0.6494 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:16:16: # Command line: callpeak -t SRX2745397.bam -f BAM -g 12100000 -n SRX2745397.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2745397.20 # format = BAM # ChIP-seq file = ['SRX2745397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:16:16: #1 read tag files... INFO @ Thu, 03 May 2018 23:16:16: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:16:16: # Command line: callpeak -t SRX2745397.bam -f BAM -g 12100000 -n SRX2745397.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2745397.10 # format = BAM # ChIP-seq file = ['SRX2745397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:16:16: #1 read tag files... INFO @ Thu, 03 May 2018 23:16:16: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:16:16: # Command line: callpeak -t SRX2745397.bam -f BAM -g 12100000 -n SRX2745397.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2745397.05 # format = BAM # ChIP-seq file = ['SRX2745397.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:16:16: #1 read tag files... INFO @ Thu, 03 May 2018 23:16:16: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:16:24: 1000000 INFO @ Thu, 03 May 2018 23:16:24: 1000000 INFO @ Thu, 03 May 2018 23:16:24: 1000000 INFO @ Thu, 03 May 2018 23:16:32: 2000000 INFO @ Thu, 03 May 2018 23:16:32: 2000000 INFO @ Thu, 03 May 2018 23:16:32: 2000000 INFO @ Thu, 03 May 2018 23:16:39: 3000000 INFO @ Thu, 03 May 2018 23:16:40: 3000000 INFO @ Thu, 03 May 2018 23:16:40: 3000000 INFO @ Thu, 03 May 2018 23:16:47: 4000000 INFO @ Thu, 03 May 2018 23:16:47: 4000000 INFO @ Thu, 03 May 2018 23:16:47: 4000000 INFO @ Thu, 03 May 2018 23:16:55: 5000000 INFO @ Thu, 03 May 2018 23:16:55: 5000000 INFO @ Thu, 03 May 2018 23:16:55: 5000000 INFO @ Thu, 03 May 2018 23:17:02: 6000000 INFO @ Thu, 03 May 2018 23:17:03: 6000000 INFO @ Thu, 03 May 2018 23:17:03: 6000000 INFO @ Thu, 03 May 2018 23:17:10: 7000000 INFO @ Thu, 03 May 2018 23:17:11: 7000000 INFO @ Thu, 03 May 2018 23:17:11: 7000000 INFO @ Thu, 03 May 2018 23:17:17: 8000000 INFO @ Thu, 03 May 2018 23:17:18: 8000000 INFO @ Thu, 03 May 2018 23:17:18: 8000000 INFO @ Thu, 03 May 2018 23:17:24: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:17:24: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:17:24: #1 total tags in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:24: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:17:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:17:24: #1 tags after filtering in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:17:24: #1 finished! INFO @ Thu, 03 May 2018 23:17:24: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:17:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:17:24: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:17:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:17:24: Process for pairing-model is terminated! cat: SRX2745397.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745397.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:17:25: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:17:25: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:17:25: #1 total tags in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:25: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:17:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:17:25: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 23:17:25: #1 tag size = 101 INFO @ Thu, 03 May 2018 23:17:25: #1 total tags in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:25: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:17:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:17:25: #1 tags after filtering in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:17:25: #1 finished! INFO @ Thu, 03 May 2018 23:17:25: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:17:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:17:25: #1 tags after filtering in treatment: 8804583 INFO @ Thu, 03 May 2018 23:17:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:17:25: #1 finished! INFO @ Thu, 03 May 2018 23:17:25: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:17:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:17:25: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:17:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:17:25: Process for pairing-model is terminated! cat: SRX2745397.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745397.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:17:25: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:17:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:17:25: Process for pairing-model is terminated! cat: SRX2745397.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745397.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745397.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。