Job ID = 10608985 sra ファイルのダウンロード中... Completed: 724109K bytes transferred in 57 seconds (103296K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 23861925 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745379/SRR5457310.sra Written 23861925 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:45 23861925 reads; of these: 23861925 (100.00%) were unpaired; of these: 3903051 (16.36%) aligned 0 times 15462615 (64.80%) aligned exactly 1 time 4496259 (18.84%) aligned >1 times 83.64% overall alignment rate Time searching: 00:04:45 Overall time: 00:04:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14264042 / 19958874 = 0.7147 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:03:37: # Command line: callpeak -t SRX2745379.bam -f BAM -g 12100000 -n SRX2745379.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2745379.10 # format = BAM # ChIP-seq file = ['SRX2745379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:03:37: #1 read tag files... INFO @ Thu, 03 May 2018 23:03:37: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:03:37: # Command line: callpeak -t SRX2745379.bam -f BAM -g 12100000 -n SRX2745379.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2745379.05 # format = BAM # ChIP-seq file = ['SRX2745379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:03:37: #1 read tag files... INFO @ Thu, 03 May 2018 23:03:37: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:03:37: # Command line: callpeak -t SRX2745379.bam -f BAM -g 12100000 -n SRX2745379.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2745379.20 # format = BAM # ChIP-seq file = ['SRX2745379.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:03:37: #1 read tag files... INFO @ Thu, 03 May 2018 23:03:37: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:03:44: 1000000 INFO @ Thu, 03 May 2018 23:03:44: 1000000 INFO @ Thu, 03 May 2018 23:03:44: 1000000 INFO @ Thu, 03 May 2018 23:03:51: 2000000 INFO @ Thu, 03 May 2018 23:03:51: 2000000 INFO @ Thu, 03 May 2018 23:03:51: 2000000 INFO @ Thu, 03 May 2018 23:03:57: 3000000 INFO @ Thu, 03 May 2018 23:03:57: 3000000 INFO @ Thu, 03 May 2018 23:03:58: 3000000 INFO @ Thu, 03 May 2018 23:04:05: 4000000 INFO @ Thu, 03 May 2018 23:04:05: 4000000 INFO @ Thu, 03 May 2018 23:04:06: 4000000 INFO @ Thu, 03 May 2018 23:04:12: 5000000 INFO @ Thu, 03 May 2018 23:04:12: 5000000 INFO @ Thu, 03 May 2018 23:04:14: 5000000 INFO @ Thu, 03 May 2018 23:04:17: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:04:17: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:04:17: #1 total tags in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:17: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:04:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:04:17: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:04:17: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:04:17: #1 total tags in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:17: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:04:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:04:17: #1 tags after filtering in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:04:17: #1 finished! INFO @ Thu, 03 May 2018 23:04:17: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:04:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:04:17: #1 tags after filtering in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:04:17: #1 finished! INFO @ Thu, 03 May 2018 23:04:17: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:04:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:04:18: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:04:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:04:18: Process for pairing-model is terminated! cat: SRX2745379.20_peaks.narrowPeakINFO @ Thu, 03 May 2018 23:04:18: #2 number of paired peaks: 0 : そのようなファイルやディレクトリはありませんWARNING @ Thu, 03 May 2018 23:04:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:04:18: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis cat: SRX2745379.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745379.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745379.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:04:19: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:04:19: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:04:19: #1 total tags in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:19: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:04:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:04:19: #1 tags after filtering in treatment: 5694832 INFO @ Thu, 03 May 2018 23:04:19: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:04:19: #1 finished! INFO @ Thu, 03 May 2018 23:04:19: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:04:19: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:04:20: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:04:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:04:20: Process for pairing-model is terminated! cat: SRX2745379.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745379.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745379.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。