Job ID = 10608982 sra ファイルのダウンロード中... Completed: 1442704K bytes transferred in 100 seconds (117379K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 45888952 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2745376/SRR5457307.sra Written 45888952 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:17 45888952 reads; of these: 45888952 (100.00%) were unpaired; of these: 12142866 (26.46%) aligned 0 times 13174514 (28.71%) aligned exactly 1 time 20571572 (44.83%) aligned >1 times 73.54% overall alignment rate Time searching: 00:15:17 Overall time: 00:15:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 29162216 / 33746086 = 0.8642 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:17:46: # Command line: callpeak -t SRX2745376.bam -f BAM -g 12100000 -n SRX2745376.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2745376.20 # format = BAM # ChIP-seq file = ['SRX2745376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:17:46: #1 read tag files... INFO @ Thu, 03 May 2018 23:17:46: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:17:46: # Command line: callpeak -t SRX2745376.bam -f BAM -g 12100000 -n SRX2745376.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2745376.10 # format = BAM # ChIP-seq file = ['SRX2745376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:17:46: #1 read tag files... INFO @ Thu, 03 May 2018 23:17:46: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:17:46: # Command line: callpeak -t SRX2745376.bam -f BAM -g 12100000 -n SRX2745376.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2745376.05 # format = BAM # ChIP-seq file = ['SRX2745376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:17:46: #1 read tag files... INFO @ Thu, 03 May 2018 23:17:46: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:17:56: 1000000 INFO @ Thu, 03 May 2018 23:17:56: 1000000 INFO @ Thu, 03 May 2018 23:17:56: 1000000 INFO @ Thu, 03 May 2018 23:18:06: 2000000 INFO @ Thu, 03 May 2018 23:18:06: 2000000 INFO @ Thu, 03 May 2018 23:18:06: 2000000 INFO @ Thu, 03 May 2018 23:18:15: 3000000 INFO @ Thu, 03 May 2018 23:18:15: 3000000 INFO @ Thu, 03 May 2018 23:18:15: 3000000 INFO @ Thu, 03 May 2018 23:18:25: 4000000 INFO @ Thu, 03 May 2018 23:18:25: 4000000 INFO @ Thu, 03 May 2018 23:18:25: 4000000 INFO @ Thu, 03 May 2018 23:18:31: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:18:31: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:18:31: #1 total tags in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:18:31: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:18:31: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:18:31: #1 total tags in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:18:31: #1 tag size is determined as 50 bps INFO @ Thu, 03 May 2018 23:18:31: #1 tag size = 50 INFO @ Thu, 03 May 2018 23:18:31: #1 total tags in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:18:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:18:31: #1 tags after filtering in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:18:31: #1 finished! INFO @ Thu, 03 May 2018 23:18:31: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:18:31: #1 tags after filtering in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:18:31: #1 finished! INFO @ Thu, 03 May 2018 23:18:31: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:18:31: #1 tags after filtering in treatment: 4583870 INFO @ Thu, 03 May 2018 23:18:31: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 23:18:31: #1 finished! INFO @ Thu, 03 May 2018 23:18:31: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:18:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:18:31: #2 number of paired peaks: 54 WARNING @ Thu, 03 May 2018 23:18:31: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:18:31: Process for pairing-model is terminated! INFO @ Thu, 03 May 2018 23:18:31: #2 number of paired peaks: 54 WARNING @ Thu, 03 May 2018 23:18:31: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:18:31: Process for pairing-model is terminated! cat: SRX2745376.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2745376.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745376.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745376.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745376.20_model.r'rm: : そのようなファイルやディレクトリはありませんcannot remove `SRX2745376.10_peaks.narrowPeak' : そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745376.20_*.xls': そのようなファイルやディレクトリはありません rm: CompletedMACS2peakCalling cannot remove `SRX2745376.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:18:31: #2 number of paired peaks: 54 WARNING @ Thu, 03 May 2018 23:18:31: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:18:31: Process for pairing-model is terminated! cat: SRX2745376.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2745376.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745376.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2745376.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。