Job ID = 2010069 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,006,395 reads read : 12,012,790 reads written : 6,006,395 reads 0-length : 6,006,395 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:44 6006395 reads; of these: 6006395 (100.00%) were unpaired; of these: 613037 (10.21%) aligned 0 times 4616297 (76.86%) aligned exactly 1 time 777061 (12.94%) aligned >1 times 89.79% overall alignment rate Time searching: 00:04:44 Overall time: 00:04:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1442862 / 5393358 = 0.2675 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:16:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:16:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:16:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:16:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:16:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:16:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:16:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:16:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:16:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:16:47: 1000000 INFO @ Fri, 05 Jul 2019 21:16:47: 1000000 INFO @ Fri, 05 Jul 2019 21:16:49: 1000000 INFO @ Fri, 05 Jul 2019 21:16:56: 2000000 INFO @ Fri, 05 Jul 2019 21:16:57: 2000000 INFO @ Fri, 05 Jul 2019 21:16:59: 2000000 INFO @ Fri, 05 Jul 2019 21:17:05: 3000000 INFO @ Fri, 05 Jul 2019 21:17:07: 3000000 INFO @ Fri, 05 Jul 2019 21:17:09: 3000000 INFO @ Fri, 05 Jul 2019 21:17:13: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 21:17:13: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 21:17:13: #1 total tags in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:13: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:17:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:17:13: #1 tags after filtering in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:17:13: #1 finished! INFO @ Fri, 05 Jul 2019 21:17:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:17:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:17:13: #2 number of paired peaks: 83 WARNING @ Fri, 05 Jul 2019 21:17:13: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:17:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:17:17: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 21:17:17: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 21:17:17: #1 total tags in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:17: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:17:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:17:17: #1 tags after filtering in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:17:17: #1 finished! INFO @ Fri, 05 Jul 2019 21:17:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:17:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:17:17: #2 number of paired peaks: 83 WARNING @ Fri, 05 Jul 2019 21:17:17: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:17:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:17:18: #1 tag size is determined as 126 bps INFO @ Fri, 05 Jul 2019 21:17:18: #1 tag size = 126 INFO @ Fri, 05 Jul 2019 21:17:18: #1 total tags in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:17:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:17:18: #1 tags after filtering in treatment: 3950496 INFO @ Fri, 05 Jul 2019 21:17:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:17:18: #1 finished! INFO @ Fri, 05 Jul 2019 21:17:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:17:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:17:19: #2 number of paired peaks: 83 WARNING @ Fri, 05 Jul 2019 21:17:19: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:17:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737662/SRX2737662.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。