Job ID = 2010063 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T12:09:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T12:09:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 5,614,356 reads read : 5,614,356 reads written : 5,614,356 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:51 5614356 reads; of these: 5614356 (100.00%) were unpaired; of these: 9790 (0.17%) aligned 0 times 4896281 (87.21%) aligned exactly 1 time 708285 (12.62%) aligned >1 times 99.83% overall alignment rate Time searching: 00:00:51 Overall time: 00:00:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1685412 / 5604566 = 0.3007 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:11:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:29: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:29: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:34: 1000000 INFO @ Fri, 05 Jul 2019 21:11:36: 1000000 INFO @ Fri, 05 Jul 2019 21:11:37: 1000000 INFO @ Fri, 05 Jul 2019 21:11:40: 2000000 INFO @ Fri, 05 Jul 2019 21:11:43: 2000000 INFO @ Fri, 05 Jul 2019 21:11:46: 2000000 INFO @ Fri, 05 Jul 2019 21:11:47: 3000000 INFO @ Fri, 05 Jul 2019 21:11:50: 3000000 INFO @ Fri, 05 Jul 2019 21:11:53: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:11:53: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:11:53: #1 total tags in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:11:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:11:53: #1 tags after filtering in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:11:53: #1 finished! INFO @ Fri, 05 Jul 2019 21:11:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:11:53: #2 number of paired peaks: 41 WARNING @ Fri, 05 Jul 2019 21:11:53: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:11:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:11:54: 3000000 INFO @ Fri, 05 Jul 2019 21:11:56: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:11:56: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:11:56: #1 total tags in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:11:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:11:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:11:56: #1 tags after filtering in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:11:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:11:56: #1 finished! INFO @ Fri, 05 Jul 2019 21:11:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:11:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:11:57: #2 number of paired peaks: 41 WARNING @ Fri, 05 Jul 2019 21:11:57: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:11:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:12:02: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:12:02: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:12:02: #1 total tags in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:12:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:12:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:12:02: #1 tags after filtering in treatment: 3919154 INFO @ Fri, 05 Jul 2019 21:12:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:12:02: #1 finished! INFO @ Fri, 05 Jul 2019 21:12:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:12:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:12:02: #2 number of paired peaks: 41 WARNING @ Fri, 05 Jul 2019 21:12:02: Too few paired peaks (41) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:12:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2737657/SRX2737657.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。