Job ID = 10608960 sra ファイルのダウンロード中... Completed: 591998K bytes transferred in 34 seconds (138875K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 14897949 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2737499/SRR5448632.sra Written 14897949 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:05 14897949 reads; of these: 14897949 (100.00%) were unpaired; of these: 274438 (1.84%) aligned 0 times 12216347 (82.00%) aligned exactly 1 time 2407164 (16.16%) aligned >1 times 98.16% overall alignment rate Time searching: 00:04:05 Overall time: 00:04:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5108943 / 14623511 = 0.3494 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 22:55:58: # Command line: callpeak -t SRX2737499.bam -f BAM -g 12100000 -n SRX2737499.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2737499.05 # format = BAM # ChIP-seq file = ['SRX2737499.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:55:58: #1 read tag files... INFO @ Thu, 03 May 2018 22:55:58: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:55:58: # Command line: callpeak -t SRX2737499.bam -f BAM -g 12100000 -n SRX2737499.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2737499.20 # format = BAM # ChIP-seq file = ['SRX2737499.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:55:58: #1 read tag files... INFO @ Thu, 03 May 2018 22:55:58: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:55:58: # Command line: callpeak -t SRX2737499.bam -f BAM -g 12100000 -n SRX2737499.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2737499.10 # format = BAM # ChIP-seq file = ['SRX2737499.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:55:58: #1 read tag files... INFO @ Thu, 03 May 2018 22:55:58: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:56:05: 1000000 INFO @ Thu, 03 May 2018 22:56:05: 1000000 INFO @ Thu, 03 May 2018 22:56:05: 1000000 INFO @ Thu, 03 May 2018 22:56:13: 2000000 INFO @ Thu, 03 May 2018 22:56:13: 2000000 INFO @ Thu, 03 May 2018 22:56:13: 2000000 INFO @ Thu, 03 May 2018 22:56:20: 3000000 INFO @ Thu, 03 May 2018 22:56:20: 3000000 INFO @ Thu, 03 May 2018 22:56:20: 3000000 INFO @ Thu, 03 May 2018 22:56:27: 4000000 INFO @ Thu, 03 May 2018 22:56:27: 4000000 INFO @ Thu, 03 May 2018 22:56:28: 4000000 INFO @ Thu, 03 May 2018 22:56:35: 5000000 INFO @ Thu, 03 May 2018 22:56:35: 5000000 INFO @ Thu, 03 May 2018 22:56:35: 5000000 INFO @ Thu, 03 May 2018 22:56:42: 6000000 INFO @ Thu, 03 May 2018 22:56:42: 6000000 INFO @ Thu, 03 May 2018 22:56:43: 6000000 INFO @ Thu, 03 May 2018 22:56:50: 7000000 INFO @ Thu, 03 May 2018 22:56:50: 7000000 INFO @ Thu, 03 May 2018 22:56:50: 7000000 INFO @ Thu, 03 May 2018 22:56:57: 8000000 INFO @ Thu, 03 May 2018 22:56:57: 8000000 INFO @ Thu, 03 May 2018 22:56:58: 8000000 INFO @ Thu, 03 May 2018 22:57:05: 9000000 INFO @ Thu, 03 May 2018 22:57:05: 9000000 INFO @ Thu, 03 May 2018 22:57:05: 9000000 INFO @ Thu, 03 May 2018 22:57:08: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:57:08: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:57:08: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:57:08: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:57:08: #1 total tags in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:08: #1 total tags in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:08: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:57:08: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:57:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:57:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:57:09: #1 tags after filtering in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:09: #1 tags after filtering in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:57:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:57:09: #1 finished! INFO @ Thu, 03 May 2018 22:57:09: #1 finished! INFO @ Thu, 03 May 2018 22:57:09: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:57:09: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:57:09: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:57:09: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:57:09: #1 total tags in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:09: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:57:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:57:09: #1 tags after filtering in treatment: 9514568 INFO @ Thu, 03 May 2018 22:57:09: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:57:09: #1 finished! INFO @ Thu, 03 May 2018 22:57:09: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:57:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:57:09: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:57:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:57:09: Process for pairing-model is terminated! INFO @ Thu, 03 May 2018 22:57:09: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:57:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:57:09: Process for pairing-model is terminated! cat: SRX2737499.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2737499.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737499.20_model.r'rm: : そのようなファイルやディレクトリはありませんcannot remove `SRX2737499.05_model.r' : そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 22:57:10: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:57:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:57:10: Process for pairing-model is terminated! cat: SRX2737499.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737499.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737499.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。