Job ID = 10608919 sra ファイルのダウンロード中... Completed: 505878K bytes transferred in 28 seconds (147722K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 12652936 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2737490/SRR5448623.sra Written 12652936 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:27 12652936 reads; of these: 12652936 (100.00%) were unpaired; of these: 1033054 (8.16%) aligned 0 times 9435762 (74.57%) aligned exactly 1 time 2184120 (17.26%) aligned >1 times 91.84% overall alignment rate Time searching: 00:03:27 Overall time: 00:03:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4207424 / 11619882 = 0.3621 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 22:51:47: # Command line: callpeak -t SRX2737490.bam -f BAM -g 12100000 -n SRX2737490.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2737490.10 # format = BAM # ChIP-seq file = ['SRX2737490.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:51:47: #1 read tag files... INFO @ Thu, 03 May 2018 22:51:47: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:51:47: # Command line: callpeak -t SRX2737490.bam -f BAM -g 12100000 -n SRX2737490.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2737490.05 # format = BAM # ChIP-seq file = ['SRX2737490.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:51:47: #1 read tag files... INFO @ Thu, 03 May 2018 22:51:47: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:51:47: # Command line: callpeak -t SRX2737490.bam -f BAM -g 12100000 -n SRX2737490.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2737490.20 # format = BAM # ChIP-seq file = ['SRX2737490.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:51:47: #1 read tag files... INFO @ Thu, 03 May 2018 22:51:47: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:51:54: 1000000 INFO @ Thu, 03 May 2018 22:51:55: 1000000 INFO @ Thu, 03 May 2018 22:51:55: 1000000 INFO @ Thu, 03 May 2018 22:52:02: 2000000 INFO @ Thu, 03 May 2018 22:52:02: 2000000 INFO @ Thu, 03 May 2018 22:52:02: 2000000 INFO @ Thu, 03 May 2018 22:52:09: 3000000 INFO @ Thu, 03 May 2018 22:52:10: 3000000 INFO @ Thu, 03 May 2018 22:52:11: 3000000 INFO @ Thu, 03 May 2018 22:52:17: 4000000 INFO @ Thu, 03 May 2018 22:52:17: 4000000 INFO @ Thu, 03 May 2018 22:52:19: 4000000 INFO @ Thu, 03 May 2018 22:52:25: 5000000 INFO @ Thu, 03 May 2018 22:52:25: 5000000 INFO @ Thu, 03 May 2018 22:52:28: 5000000 INFO @ Thu, 03 May 2018 22:52:33: 6000000 INFO @ Thu, 03 May 2018 22:52:34: 6000000 INFO @ Thu, 03 May 2018 22:52:37: 6000000 INFO @ Thu, 03 May 2018 22:52:41: 7000000 INFO @ Thu, 03 May 2018 22:52:42: 7000000 INFO @ Thu, 03 May 2018 22:52:44: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:52:44: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:52:44: #1 total tags in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:44: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:52:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:52:44: #1 tags after filtering in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:44: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:52:44: #1 finished! INFO @ Thu, 03 May 2018 22:52:44: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:52:44: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:52:45: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:52:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:52:45: Process for pairing-model is terminated! cat: SRX2737490.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737490.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 22:52:45: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:52:45: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:52:45: #1 total tags in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:45: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:52:45: #1 tags after filtering in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:52:45: #1 finished! INFO @ Thu, 03 May 2018 22:52:45: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:52:46: 7000000 INFO @ Thu, 03 May 2018 22:52:46: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:52:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:52:46: Process for pairing-model is terminated! cat: SRX2737490.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737490.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 22:52:49: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:52:49: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:52:49: #1 total tags in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:49: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:52:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:52:49: #1 tags after filtering in treatment: 7412458 INFO @ Thu, 03 May 2018 22:52:49: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:52:49: #1 finished! INFO @ Thu, 03 May 2018 22:52:49: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:52:49: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:52:49: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:52:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:52:49: Process for pairing-model is terminated! cat: SRX2737490.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737490.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737490.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。