Job ID = 10608917 sra ファイルのダウンロード中... Completed: 459334K bytes transferred in 20 seconds (183762K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 11427913 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2737488/SRR5448621.sra Written 11427913 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:17 11427913 reads; of these: 11427913 (100.00%) were unpaired; of these: 679651 (5.95%) aligned 0 times 8127840 (71.12%) aligned exactly 1 time 2620422 (22.93%) aligned >1 times 94.05% overall alignment rate Time searching: 00:03:17 Overall time: 00:03:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4227610 / 10748262 = 0.3933 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 22:50:08: # Command line: callpeak -t SRX2737488.bam -f BAM -g 12100000 -n SRX2737488.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2737488.20 # format = BAM # ChIP-seq file = ['SRX2737488.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:50:08: #1 read tag files... INFO @ Thu, 03 May 2018 22:50:08: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:50:08: # Command line: callpeak -t SRX2737488.bam -f BAM -g 12100000 -n SRX2737488.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2737488.05 # format = BAM # ChIP-seq file = ['SRX2737488.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:50:08: #1 read tag files... INFO @ Thu, 03 May 2018 22:50:08: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:50:08: # Command line: callpeak -t SRX2737488.bam -f BAM -g 12100000 -n SRX2737488.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2737488.10 # format = BAM # ChIP-seq file = ['SRX2737488.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 22:50:08: #1 read tag files... INFO @ Thu, 03 May 2018 22:50:08: #1 read treatment tags... INFO @ Thu, 03 May 2018 22:50:18: 1000000 INFO @ Thu, 03 May 2018 22:50:19: 1000000 INFO @ Thu, 03 May 2018 22:50:19: 1000000 INFO @ Thu, 03 May 2018 22:50:27: 2000000 INFO @ Thu, 03 May 2018 22:50:31: 2000000 INFO @ Thu, 03 May 2018 22:50:31: 2000000 INFO @ Thu, 03 May 2018 22:50:37: 3000000 INFO @ Thu, 03 May 2018 22:50:42: 3000000 INFO @ Thu, 03 May 2018 22:50:42: 3000000 INFO @ Thu, 03 May 2018 22:50:47: 4000000 INFO @ Thu, 03 May 2018 22:50:53: 4000000 INFO @ Thu, 03 May 2018 22:50:53: 4000000 INFO @ Thu, 03 May 2018 22:50:57: 5000000 INFO @ Thu, 03 May 2018 22:51:05: 5000000 INFO @ Thu, 03 May 2018 22:51:05: 5000000 INFO @ Thu, 03 May 2018 22:51:06: 6000000 INFO @ Thu, 03 May 2018 22:51:11: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:51:11: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:51:11: #1 total tags in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:11: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:51:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:51:11: #1 tags after filtering in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:51:11: #1 finished! INFO @ Thu, 03 May 2018 22:51:11: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:51:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:51:12: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:51:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:51:12: Process for pairing-model is terminated! cat: SRX2737488.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737488.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 22:51:17: 6000000 INFO @ Thu, 03 May 2018 22:51:17: 6000000 INFO @ Thu, 03 May 2018 22:51:22: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:51:22: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:51:22: #1 total tags in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:22: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:51:22: #1 tags after filtering in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:51:22: #1 finished! INFO @ Thu, 03 May 2018 22:51:22: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:51:22: #1 tag size is determined as 101 bps INFO @ Thu, 03 May 2018 22:51:22: #1 tag size = 101 INFO @ Thu, 03 May 2018 22:51:22: #1 total tags in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:22: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 22:51:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 22:51:22: #1 tags after filtering in treatment: 6520652 INFO @ Thu, 03 May 2018 22:51:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 03 May 2018 22:51:22: #1 finished! INFO @ Thu, 03 May 2018 22:51:22: #2 Build Peak Model... INFO @ Thu, 03 May 2018 22:51:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 22:51:22: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:51:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:51:22: Process for pairing-model is terminated! cat: SRX2737488.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737488.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 22:51:23: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 22:51:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 22:51:23: Process for pairing-model is terminated! cat: SRX2737488.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737488.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737488.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。