Job ID = 9160132 sra ファイルのダウンロード中... Completed: 456277K bytes transferred in 7 seconds (494080K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 20675038 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2619076/SRR5319661.sra Written 20675038 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:33 20675038 reads; of these: 20675038 (100.00%) were unpaired; of these: 355240 (1.72%) aligned 0 times 17122504 (82.82%) aligned exactly 1 time 3197294 (15.46%) aligned >1 times 98.28% overall alignment rate Time searching: 00:03:33 Overall time: 00:03:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8804356 / 20319798 = 0.4333 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 02:03:44: # Command line: callpeak -t SRX2619076.bam -f BAM -g 12100000 -n SRX2619076.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2619076.10 # format = BAM # ChIP-seq file = ['SRX2619076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:44: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:44: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:44: # Command line: callpeak -t SRX2619076.bam -f BAM -g 12100000 -n SRX2619076.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2619076.05 # format = BAM # ChIP-seq file = ['SRX2619076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:44: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:44: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:44: # Command line: callpeak -t SRX2619076.bam -f BAM -g 12100000 -n SRX2619076.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2619076.20 # format = BAM # ChIP-seq file = ['SRX2619076.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:03:44: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:03:44: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:03:50: 1000000 INFO @ Wed, 28 Jun 2017 02:03:50: 1000000 INFO @ Wed, 28 Jun 2017 02:03:50: 1000000 INFO @ Wed, 28 Jun 2017 02:03:56: 2000000 INFO @ Wed, 28 Jun 2017 02:03:56: 2000000 INFO @ Wed, 28 Jun 2017 02:03:56: 2000000 INFO @ Wed, 28 Jun 2017 02:04:02: 3000000 INFO @ Wed, 28 Jun 2017 02:04:02: 3000000 INFO @ Wed, 28 Jun 2017 02:04:02: 3000000 INFO @ Wed, 28 Jun 2017 02:04:08: 4000000 INFO @ Wed, 28 Jun 2017 02:04:08: 4000000 INFO @ Wed, 28 Jun 2017 02:04:09: 4000000 INFO @ Wed, 28 Jun 2017 02:04:14: 5000000 INFO @ Wed, 28 Jun 2017 02:04:14: 5000000 INFO @ Wed, 28 Jun 2017 02:04:15: 5000000 INFO @ Wed, 28 Jun 2017 02:04:20: 6000000 INFO @ Wed, 28 Jun 2017 02:04:20: 6000000 INFO @ Wed, 28 Jun 2017 02:04:22: 6000000 INFO @ Wed, 28 Jun 2017 02:04:26: 7000000 INFO @ Wed, 28 Jun 2017 02:04:27: 7000000 INFO @ Wed, 28 Jun 2017 02:04:29: 7000000 INFO @ Wed, 28 Jun 2017 02:04:33: 8000000 INFO @ Wed, 28 Jun 2017 02:04:33: 8000000 INFO @ Wed, 28 Jun 2017 02:04:35: 8000000 INFO @ Wed, 28 Jun 2017 02:04:39: 9000000 INFO @ Wed, 28 Jun 2017 02:04:40: 9000000 INFO @ Wed, 28 Jun 2017 02:04:42: 9000000 INFO @ Wed, 28 Jun 2017 02:04:45: 10000000 INFO @ Wed, 28 Jun 2017 02:04:46: 10000000 INFO @ Wed, 28 Jun 2017 02:04:49: 10000000 INFO @ Wed, 28 Jun 2017 02:04:51: 11000000 INFO @ Wed, 28 Jun 2017 02:04:52: 11000000 INFO @ Wed, 28 Jun 2017 02:04:55: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:55: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:55: #1 total tags in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:55: #1 tags after filtering in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:55: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:55: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:55: 11000000 INFO @ Wed, 28 Jun 2017 02:04:55: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:55: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:55: #1 total tags in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:55: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:55: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:55: Process for pairing-model is terminated! cat: SRX2619076.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619076.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:04:56: #1 tags after filtering in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:56: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:56: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:56: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:56: Process for pairing-model is terminated! cat: SRX2619076.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619076.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:04:58: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:04:58: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:04:58: #1 total tags in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:58: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:04:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:04:59: #1 tags after filtering in treatment: 11515442 INFO @ Wed, 28 Jun 2017 02:04:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:04:59: #1 finished! INFO @ Wed, 28 Jun 2017 02:04:59: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:04:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:04:59: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:04:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:04:59: Process for pairing-model is terminated! cat: SRX2619076.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619076.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619076.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。