Job ID = 9160124 sra ファイルのダウンロード中... Completed: 629983K bytes transferred in 11 seconds (439327K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28616821 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2619068/SRR5319653.sra Written 28616821 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:19 28616821 reads; of these: 28616821 (100.00%) were unpaired; of these: 675728 (2.36%) aligned 0 times 24411451 (85.30%) aligned exactly 1 time 3529642 (12.33%) aligned >1 times 97.64% overall alignment rate Time searching: 00:05:19 Overall time: 00:05:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14116545 / 27941093 = 0.5052 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 02:06:06: # Command line: callpeak -t SRX2619068.bam -f BAM -g 12100000 -n SRX2619068.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2619068.05 # format = BAM # ChIP-seq file = ['SRX2619068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:06:06: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:06:06: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:06:06: # Command line: callpeak -t SRX2619068.bam -f BAM -g 12100000 -n SRX2619068.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2619068.20 # format = BAM # ChIP-seq file = ['SRX2619068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:06:06: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:06:06: # Command line: callpeak -t SRX2619068.bam -f BAM -g 12100000 -n SRX2619068.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2619068.10 # format = BAM # ChIP-seq file = ['SRX2619068.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:06:06: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:06:06: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:06:06: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:06:15: 1000000 INFO @ Wed, 28 Jun 2017 02:06:15: 1000000 INFO @ Wed, 28 Jun 2017 02:06:15: 1000000 INFO @ Wed, 28 Jun 2017 02:06:23: 2000000 INFO @ Wed, 28 Jun 2017 02:06:23: 2000000 INFO @ Wed, 28 Jun 2017 02:06:23: 2000000 INFO @ Wed, 28 Jun 2017 02:06:30: 3000000 INFO @ Wed, 28 Jun 2017 02:06:31: 3000000 INFO @ Wed, 28 Jun 2017 02:06:31: 3000000 INFO @ Wed, 28 Jun 2017 02:06:38: 4000000 INFO @ Wed, 28 Jun 2017 02:06:38: 4000000 INFO @ Wed, 28 Jun 2017 02:06:38: 4000000 INFO @ Wed, 28 Jun 2017 02:06:46: 5000000 INFO @ Wed, 28 Jun 2017 02:06:46: 5000000 INFO @ Wed, 28 Jun 2017 02:06:46: 5000000 INFO @ Wed, 28 Jun 2017 02:06:54: 6000000 INFO @ Wed, 28 Jun 2017 02:06:54: 6000000 INFO @ Wed, 28 Jun 2017 02:06:55: 6000000 INFO @ Wed, 28 Jun 2017 02:07:03: 7000000 INFO @ Wed, 28 Jun 2017 02:07:03: 7000000 INFO @ Wed, 28 Jun 2017 02:07:04: 7000000 INFO @ Wed, 28 Jun 2017 02:07:09: 8000000 INFO @ Wed, 28 Jun 2017 02:07:12: 8000000 INFO @ Wed, 28 Jun 2017 02:07:14: 8000000 INFO @ Wed, 28 Jun 2017 02:07:16: 9000000 INFO @ Wed, 28 Jun 2017 02:07:22: 9000000 INFO @ Wed, 28 Jun 2017 02:07:23: 9000000 INFO @ Wed, 28 Jun 2017 02:07:23: 10000000 INFO @ Wed, 28 Jun 2017 02:07:30: 11000000 INFO @ Wed, 28 Jun 2017 02:07:31: 10000000 INFO @ Wed, 28 Jun 2017 02:07:32: 10000000 INFO @ Wed, 28 Jun 2017 02:07:37: 12000000 INFO @ Wed, 28 Jun 2017 02:07:40: 11000000 INFO @ Wed, 28 Jun 2017 02:07:41: 11000000 INFO @ Wed, 28 Jun 2017 02:07:44: 13000000 INFO @ Wed, 28 Jun 2017 02:07:48: 12000000 INFO @ Wed, 28 Jun 2017 02:07:49: 12000000 INFO @ Wed, 28 Jun 2017 02:07:50: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:07:50: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:07:50: #1 total tags in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:07:50: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:07:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:07:51: #1 tags after filtering in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:07:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:07:51: #1 finished! INFO @ Wed, 28 Jun 2017 02:07:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:07:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:07:51: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:07:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:07:51: Process for pairing-model is terminated! cat: SRX2619068.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619068.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:07:55: 13000000 INFO @ Wed, 28 Jun 2017 02:07:56: 13000000 INFO @ Wed, 28 Jun 2017 02:08:01: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:08:01: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:08:01: #1 total tags in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:08:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:08:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:08:01: #1 tags after filtering in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:08:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:08:01: #1 finished! INFO @ Wed, 28 Jun 2017 02:08:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:08:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:08:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:08:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:08:02: Process for pairing-model is terminated! cat: SRX2619068.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619068.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:08:02: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:08:02: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:08:02: #1 total tags in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:08:02: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:08:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:08:03: #1 tags after filtering in treatment: 13824548 INFO @ Wed, 28 Jun 2017 02:08:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:08:03: #1 finished! INFO @ Wed, 28 Jun 2017 02:08:03: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:08:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:08:03: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:08:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:08:03: Process for pairing-model is terminated! cat: SRX2619068.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619068.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619068.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。