Job ID = 9160120 sra ファイルのダウンロード中... Completed: 331259K bytes transferred in 8 seconds (313746K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 14926301 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2619064/SRR5319649.sra Written 14926301 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:35 14926301 reads; of these: 14926301 (100.00%) were unpaired; of these: 381400 (2.56%) aligned 0 times 12739690 (85.35%) aligned exactly 1 time 1805211 (12.09%) aligned >1 times 97.44% overall alignment rate Time searching: 00:02:35 Overall time: 00:02:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5473845 / 14544901 = 0.3763 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 02:00:20: # Command line: callpeak -t SRX2619064.bam -f BAM -g 12100000 -n SRX2619064.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2619064.05 # format = BAM # ChIP-seq file = ['SRX2619064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:00:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:00:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:00:20: # Command line: callpeak -t SRX2619064.bam -f BAM -g 12100000 -n SRX2619064.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2619064.20 # format = BAM # ChIP-seq file = ['SRX2619064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:00:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:00:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:00:20: # Command line: callpeak -t SRX2619064.bam -f BAM -g 12100000 -n SRX2619064.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2619064.10 # format = BAM # ChIP-seq file = ['SRX2619064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:00:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:00:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:00:25: 1000000 INFO @ Wed, 28 Jun 2017 02:00:25: 1000000 INFO @ Wed, 28 Jun 2017 02:00:27: 1000000 INFO @ Wed, 28 Jun 2017 02:00:31: 2000000 INFO @ Wed, 28 Jun 2017 02:00:31: 2000000 INFO @ Wed, 28 Jun 2017 02:00:35: 2000000 INFO @ Wed, 28 Jun 2017 02:00:37: 3000000 INFO @ Wed, 28 Jun 2017 02:00:37: 3000000 INFO @ Wed, 28 Jun 2017 02:00:42: 4000000 INFO @ Wed, 28 Jun 2017 02:00:42: 4000000 INFO @ Wed, 28 Jun 2017 02:00:43: 3000000 INFO @ Wed, 28 Jun 2017 02:00:48: 5000000 INFO @ Wed, 28 Jun 2017 02:00:48: 5000000 INFO @ Wed, 28 Jun 2017 02:00:50: 4000000 INFO @ Wed, 28 Jun 2017 02:00:54: 6000000 INFO @ Wed, 28 Jun 2017 02:00:54: 6000000 INFO @ Wed, 28 Jun 2017 02:00:57: 5000000 INFO @ Wed, 28 Jun 2017 02:00:59: 7000000 INFO @ Wed, 28 Jun 2017 02:01:00: 7000000 INFO @ Wed, 28 Jun 2017 02:01:03: 6000000 INFO @ Wed, 28 Jun 2017 02:01:05: 8000000 INFO @ Wed, 28 Jun 2017 02:01:05: 8000000 INFO @ Wed, 28 Jun 2017 02:01:09: 7000000 INFO @ Wed, 28 Jun 2017 02:01:11: 9000000 INFO @ Wed, 28 Jun 2017 02:01:11: 9000000 INFO @ Wed, 28 Jun 2017 02:01:11: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:01:11: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:01:11: #1 total tags in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:11: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:01:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:01:11: #1 tags after filtering in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:01:11: #1 finished! INFO @ Wed, 28 Jun 2017 02:01:11: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:01:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:01:12: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:01:12: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:01:12: #1 total tags in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:12: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:01:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:01:12: #1 tags after filtering in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:12: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:01:12: #1 finished! INFO @ Wed, 28 Jun 2017 02:01:12: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:01:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:01:12: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:01:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:01:12: Process for pairing-model is terminated! cat: SRX2619064.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619064.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:01:12: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:01:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:01:12: Process for pairing-model is terminated! cat: SRX2619064.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619064.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:01:15: 8000000 INFO @ Wed, 28 Jun 2017 02:01:20: 9000000 INFO @ Wed, 28 Jun 2017 02:01:21: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:01:21: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:01:21: #1 total tags in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:01:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:01:21: #1 tags after filtering in treatment: 9071056 INFO @ Wed, 28 Jun 2017 02:01:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:01:21: #1 finished! INFO @ Wed, 28 Jun 2017 02:01:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:01:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:01:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:01:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:01:22: Process for pairing-model is terminated! cat: SRX2619064.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619064.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619064.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。