Job ID = 9160096 sra ファイルのダウンロード中... Completed: 103316K bytes transferred in 4 seconds (179506K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 4746551 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2619040/SRR5319625.sra Written 4746551 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:52 4746551 reads; of these: 4746551 (100.00%) were unpaired; of these: 44390 (0.94%) aligned 0 times 4154998 (87.54%) aligned exactly 1 time 547163 (11.53%) aligned >1 times 99.06% overall alignment rate Time searching: 00:00:52 Overall time: 00:00:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 753099 / 4702161 = 0.1602 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:54:13: # Command line: callpeak -t SRX2619040.bam -f BAM -g 12100000 -n SRX2619040.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2619040.20 # format = BAM # ChIP-seq file = ['SRX2619040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:54:13: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:54:13: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:54:13: # Command line: callpeak -t SRX2619040.bam -f BAM -g 12100000 -n SRX2619040.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2619040.10 # format = BAM # ChIP-seq file = ['SRX2619040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:54:13: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:54:13: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:54:13: # Command line: callpeak -t SRX2619040.bam -f BAM -g 12100000 -n SRX2619040.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2619040.05 # format = BAM # ChIP-seq file = ['SRX2619040.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:54:13: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:54:13: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:54:20: 1000000 INFO @ Wed, 28 Jun 2017 01:54:21: 1000000 INFO @ Wed, 28 Jun 2017 01:54:21: 1000000 INFO @ Wed, 28 Jun 2017 01:54:28: 2000000 INFO @ Wed, 28 Jun 2017 01:54:28: 2000000 INFO @ Wed, 28 Jun 2017 01:54:28: 2000000 INFO @ Wed, 28 Jun 2017 01:54:35: 3000000 INFO @ Wed, 28 Jun 2017 01:54:36: 3000000 INFO @ Wed, 28 Jun 2017 01:54:36: 3000000 INFO @ Wed, 28 Jun 2017 01:54:41: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:54:41: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:54:41: #1 total tags in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:54:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:54:41: #1 tags after filtering in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:54:41: #1 finished! INFO @ Wed, 28 Jun 2017 01:54:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:54:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:54:42: #2 number of paired peaks: 30 WARNING @ Wed, 28 Jun 2017 01:54:42: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:54:42: Process for pairing-model is terminated! cat: SRX2619040.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619040.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:54:43: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:54:43: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:54:43: #1 total tags in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:43: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:54:43: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:54:43: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:54:43: #1 total tags in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:43: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:54:43: #1 tags after filtering in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:54:43: #1 finished! INFO @ Wed, 28 Jun 2017 01:54:43: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:54:43: #1 tags after filtering in treatment: 3949062 INFO @ Wed, 28 Jun 2017 01:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:54:43: #1 finished! INFO @ Wed, 28 Jun 2017 01:54:43: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:54:43: #2 number of paired peaks: 30 WARNING @ Wed, 28 Jun 2017 01:54:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:54:43: Process for pairing-model is terminated! cat: SRX2619040.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619040.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:54:43: #2 number of paired peaks: 30 WARNING @ Wed, 28 Jun 2017 01:54:43: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:54:43: Process for pairing-model is terminated! cat: SRX2619040.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2619040.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2619040.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。