Job ID = 2010057 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,396,884 reads read : 27,396,884 reads written : 27,396,884 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:11 27396884 reads; of these: 27396884 (100.00%) were unpaired; of these: 1547550 (5.65%) aligned 0 times 18266428 (66.67%) aligned exactly 1 time 7582906 (27.68%) aligned >1 times 94.35% overall alignment rate Time searching: 00:05:11 Overall time: 00:05:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16454066 / 25849334 = 0.6365 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:28:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:28:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:28:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:28:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:28:50: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:28:50: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:28:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:28:51: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:28:51: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:28:57: 1000000 INFO @ Fri, 05 Jul 2019 21:28:58: 1000000 INFO @ Fri, 05 Jul 2019 21:29:00: 1000000 INFO @ Fri, 05 Jul 2019 21:29:04: 2000000 INFO @ Fri, 05 Jul 2019 21:29:05: 2000000 INFO @ Fri, 05 Jul 2019 21:29:09: 2000000 INFO @ Fri, 05 Jul 2019 21:29:11: 3000000 INFO @ Fri, 05 Jul 2019 21:29:12: 3000000 INFO @ Fri, 05 Jul 2019 21:29:17: 3000000 INFO @ Fri, 05 Jul 2019 21:29:19: 4000000 INFO @ Fri, 05 Jul 2019 21:29:20: 4000000 INFO @ Fri, 05 Jul 2019 21:29:25: 4000000 INFO @ Fri, 05 Jul 2019 21:29:26: 5000000 INFO @ Fri, 05 Jul 2019 21:29:27: 5000000 INFO @ Fri, 05 Jul 2019 21:29:33: 6000000 INFO @ Fri, 05 Jul 2019 21:29:34: 5000000 INFO @ Fri, 05 Jul 2019 21:29:34: 6000000 INFO @ Fri, 05 Jul 2019 21:29:40: 7000000 INFO @ Fri, 05 Jul 2019 21:29:42: 7000000 INFO @ Fri, 05 Jul 2019 21:29:43: 6000000 INFO @ Fri, 05 Jul 2019 21:29:48: 8000000 INFO @ Fri, 05 Jul 2019 21:29:49: 8000000 INFO @ Fri, 05 Jul 2019 21:29:51: 7000000 INFO @ Fri, 05 Jul 2019 21:29:55: 9000000 INFO @ Fri, 05 Jul 2019 21:29:56: 9000000 INFO @ Fri, 05 Jul 2019 21:29:58: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:29:58: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:29:58: #1 total tags in treatment: 9395268 INFO @ Fri, 05 Jul 2019 21:29:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:29:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:29:58: #1 tags after filtering in treatment: 9395268 INFO @ Fri, 05 Jul 2019 21:29:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:29:58: #1 finished! INFO @ Fri, 05 Jul 2019 21:29:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:29:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:29:59: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:29:59: #2 number of paired peaks: 0 INFO @ Fri, 05 Jul 2019 21:29:59: #1 tag size = 51 WARNING @ Fri, 05 Jul 2019 21:29:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Fri, 05 Jul 2019 21:29:59: #1 total tags in treatment: 9395268 WARNING @ Fri, 05 Jul 2019 21:29:59: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 21:29:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:29:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:29:59: #1 tags after filtering in treatment: 9395268 INFO @ Fri, 05 Jul 2019 21:29:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:29:59: #1 finished! INFO @ Fri, 05 Jul 2019 21:29:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:29:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:29:59: 8000000 INFO @ Fri, 05 Jul 2019 21:30:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:30:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:30:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:30:07: 9000000 INFO @ Fri, 05 Jul 2019 21:30:10: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:30:10: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:30:10: #1 total tags in treatment: 9395268 INFO @ Fri, 05 Jul 2019 21:30:10: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:30:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:30:11: #1 tags after filtering in treatment: 9395268 INFO @ Fri, 05 Jul 2019 21:30:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:30:11: #1 finished! INFO @ Fri, 05 Jul 2019 21:30:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:30:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:30:11: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:30:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:30:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609720/SRX2609720.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。