Job ID = 2010052 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 38,196,422 reads read : 38,196,422 reads written : 38,196,422 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:44 38196422 reads; of these: 38196422 (100.00%) were unpaired; of these: 2066858 (5.41%) aligned 0 times 24656689 (64.55%) aligned exactly 1 time 11472875 (30.04%) aligned >1 times 94.59% overall alignment rate Time searching: 00:06:44 Overall time: 00:06:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 24054518 / 36129564 = 0.6658 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:36:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:50: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:50: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:51: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:51: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:57: 1000000 INFO @ Fri, 05 Jul 2019 21:36:59: 1000000 INFO @ Fri, 05 Jul 2019 21:37:00: 1000000 INFO @ Fri, 05 Jul 2019 21:37:05: 2000000 INFO @ Fri, 05 Jul 2019 21:37:07: 2000000 INFO @ Fri, 05 Jul 2019 21:37:10: 2000000 INFO @ Fri, 05 Jul 2019 21:37:13: 3000000 INFO @ Fri, 05 Jul 2019 21:37:15: 3000000 INFO @ Fri, 05 Jul 2019 21:37:20: 3000000 INFO @ Fri, 05 Jul 2019 21:37:21: 4000000 INFO @ Fri, 05 Jul 2019 21:37:23: 4000000 INFO @ Fri, 05 Jul 2019 21:37:28: 5000000 INFO @ Fri, 05 Jul 2019 21:37:29: 4000000 INFO @ Fri, 05 Jul 2019 21:37:31: 5000000 INFO @ Fri, 05 Jul 2019 21:37:36: 6000000 INFO @ Fri, 05 Jul 2019 21:37:39: 5000000 INFO @ Fri, 05 Jul 2019 21:37:39: 6000000 INFO @ Fri, 05 Jul 2019 21:37:44: 7000000 INFO @ Fri, 05 Jul 2019 21:37:46: 7000000 INFO @ Fri, 05 Jul 2019 21:37:48: 6000000 INFO @ Fri, 05 Jul 2019 21:37:52: 8000000 INFO @ Fri, 05 Jul 2019 21:37:54: 8000000 INFO @ Fri, 05 Jul 2019 21:37:57: 7000000 INFO @ Fri, 05 Jul 2019 21:38:00: 9000000 INFO @ Fri, 05 Jul 2019 21:38:02: 9000000 INFO @ Fri, 05 Jul 2019 21:38:07: 8000000 INFO @ Fri, 05 Jul 2019 21:38:07: 10000000 INFO @ Fri, 05 Jul 2019 21:38:10: 10000000 INFO @ Fri, 05 Jul 2019 21:38:15: 11000000 INFO @ Fri, 05 Jul 2019 21:38:16: 9000000 INFO @ Fri, 05 Jul 2019 21:38:18: 11000000 INFO @ Fri, 05 Jul 2019 21:38:23: 12000000 INFO @ Fri, 05 Jul 2019 21:38:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:38:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:38:23: #1 total tags in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:38:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:38:24: #1 tags after filtering in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:38:24: #1 finished! INFO @ Fri, 05 Jul 2019 21:38:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:38:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:38:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:38:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:38:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:38:25: 12000000 INFO @ Fri, 05 Jul 2019 21:38:26: 10000000 INFO @ Fri, 05 Jul 2019 21:38:26: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:38:26: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:38:26: #1 total tags in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:38:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:38:26: #1 tags after filtering in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:38:26: #1 finished! INFO @ Fri, 05 Jul 2019 21:38:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:38:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:38:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:38:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:38:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:38:35: 11000000 INFO @ Fri, 05 Jul 2019 21:38:44: 12000000 INFO @ Fri, 05 Jul 2019 21:38:44: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:38:44: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:38:44: #1 total tags in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:38:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:38:45: #1 tags after filtering in treatment: 12075046 INFO @ Fri, 05 Jul 2019 21:38:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:38:45: #1 finished! INFO @ Fri, 05 Jul 2019 21:38:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:38:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:38:46: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:38:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:38:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609716/SRX2609716.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。