Job ID = 2010048 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,214,977 reads read : 9,214,977 reads written : 9,214,977 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:47 9214977 reads; of these: 9214977 (100.00%) were unpaired; of these: 543924 (5.90%) aligned 0 times 7055623 (76.57%) aligned exactly 1 time 1615430 (17.53%) aligned >1 times 94.10% overall alignment rate Time searching: 00:01:47 Overall time: 00:01:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3506669 / 8671053 = 0.4044 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:12:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:12:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:12:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:12:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:12:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:12:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:12:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:12:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:12:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:12:39: 1000000 INFO @ Fri, 05 Jul 2019 21:12:40: 1000000 INFO @ Fri, 05 Jul 2019 21:12:40: 1000000 INFO @ Fri, 05 Jul 2019 21:12:47: 2000000 INFO @ Fri, 05 Jul 2019 21:12:48: 2000000 INFO @ Fri, 05 Jul 2019 21:12:48: 2000000 INFO @ Fri, 05 Jul 2019 21:12:54: 3000000 INFO @ Fri, 05 Jul 2019 21:12:55: 3000000 INFO @ Fri, 05 Jul 2019 21:12:56: 3000000 INFO @ Fri, 05 Jul 2019 21:13:01: 4000000 INFO @ Fri, 05 Jul 2019 21:13:03: 4000000 INFO @ Fri, 05 Jul 2019 21:13:03: 4000000 INFO @ Fri, 05 Jul 2019 21:13:08: 5000000 INFO @ Fri, 05 Jul 2019 21:13:09: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:13:09: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:13:09: #1 total tags in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:13:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:13:09: #1 tags after filtering in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:13:09: #1 finished! INFO @ Fri, 05 Jul 2019 21:13:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:13:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:13:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:13:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:13:09: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 21:13:11: 5000000 INFO @ Fri, 05 Jul 2019 21:13:11: 5000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:13:12: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:13:12: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:13:12: #1 total tags in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:12: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:13:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:13:13: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:13:13: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:13:13: #1 total tags in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:13: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:13:13: #1 tags after filtering in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:13:13: #1 finished! INFO @ Fri, 05 Jul 2019 21:13:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:13:13: #1 tags after filtering in treatment: 5164384 INFO @ Fri, 05 Jul 2019 21:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:13:13: #1 finished! INFO @ Fri, 05 Jul 2019 21:13:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:13:13: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:13:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:13:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 21:13:13: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:13:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:13:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609712/SRX2609712.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。