Job ID = 2010045 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,511,322 reads read : 15,511,322 reads written : 15,511,322 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 15511322 reads; of these: 15511322 (100.00%) were unpaired; of these: 1925353 (12.41%) aligned 0 times 9224980 (59.47%) aligned exactly 1 time 4360989 (28.11%) aligned >1 times 87.59% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6164524 / 13585969 = 0.4537 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:21:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:46: 1000000 INFO @ Fri, 05 Jul 2019 21:21:47: 1000000 INFO @ Fri, 05 Jul 2019 21:21:47: 1000000 INFO @ Fri, 05 Jul 2019 21:21:53: 2000000 INFO @ Fri, 05 Jul 2019 21:21:55: 2000000 INFO @ Fri, 05 Jul 2019 21:21:56: 2000000 INFO @ Fri, 05 Jul 2019 21:22:00: 3000000 INFO @ Fri, 05 Jul 2019 21:22:05: 3000000 INFO @ Fri, 05 Jul 2019 21:22:05: 3000000 INFO @ Fri, 05 Jul 2019 21:22:07: 4000000 INFO @ Fri, 05 Jul 2019 21:22:13: 5000000 INFO @ Fri, 05 Jul 2019 21:22:14: 4000000 INFO @ Fri, 05 Jul 2019 21:22:15: 4000000 INFO @ Fri, 05 Jul 2019 21:22:20: 6000000 INFO @ Fri, 05 Jul 2019 21:22:23: 5000000 INFO @ Fri, 05 Jul 2019 21:22:24: 5000000 INFO @ Fri, 05 Jul 2019 21:22:26: 7000000 INFO @ Fri, 05 Jul 2019 21:22:29: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:29: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:29: #1 total tags in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:29: #1 tags after filtering in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:29: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:30: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:22:32: 6000000 INFO @ Fri, 05 Jul 2019 21:22:34: 6000000 INFO @ Fri, 05 Jul 2019 21:22:41: 7000000 INFO @ Fri, 05 Jul 2019 21:22:43: 7000000 INFO @ Fri, 05 Jul 2019 21:22:44: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:44: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:44: #1 total tags in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:45: #1 tags after filtering in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:45: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:45: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:22:47: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:47: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:47: #1 total tags in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:47: #1 tags after filtering in treatment: 7421445 INFO @ Fri, 05 Jul 2019 21:22:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:47: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:47: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609709/SRX2609709.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。