Job ID = 2010041 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,473,073 reads read : 19,473,073 reads written : 19,473,073 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:55 19473073 reads; of these: 19473073 (100.00%) were unpaired; of these: 194050 (1.00%) aligned 0 times 15512674 (79.66%) aligned exactly 1 time 3766349 (19.34%) aligned >1 times 99.00% overall alignment rate Time searching: 00:04:55 Overall time: 00:04:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8757092 / 19279023 = 0.4542 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:21:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:27: 1000000 INFO @ Fri, 05 Jul 2019 21:21:28: 1000000 INFO @ Fri, 05 Jul 2019 21:21:29: 1000000 INFO @ Fri, 05 Jul 2019 21:21:34: 2000000 INFO @ Fri, 05 Jul 2019 21:21:35: 2000000 INFO @ Fri, 05 Jul 2019 21:21:37: 2000000 INFO @ Fri, 05 Jul 2019 21:21:41: 3000000 INFO @ Fri, 05 Jul 2019 21:21:42: 3000000 INFO @ Fri, 05 Jul 2019 21:21:44: 3000000 INFO @ Fri, 05 Jul 2019 21:21:48: 4000000 INFO @ Fri, 05 Jul 2019 21:21:49: 4000000 INFO @ Fri, 05 Jul 2019 21:21:51: 4000000 INFO @ Fri, 05 Jul 2019 21:21:55: 5000000 INFO @ Fri, 05 Jul 2019 21:21:56: 5000000 INFO @ Fri, 05 Jul 2019 21:21:59: 5000000 INFO @ Fri, 05 Jul 2019 21:22:01: 6000000 INFO @ Fri, 05 Jul 2019 21:22:03: 6000000 INFO @ Fri, 05 Jul 2019 21:22:06: 6000000 INFO @ Fri, 05 Jul 2019 21:22:08: 7000000 INFO @ Fri, 05 Jul 2019 21:22:09: 7000000 INFO @ Fri, 05 Jul 2019 21:22:14: 7000000 INFO @ Fri, 05 Jul 2019 21:22:17: 8000000 INFO @ Fri, 05 Jul 2019 21:22:17: 8000000 INFO @ Fri, 05 Jul 2019 21:22:22: 8000000 INFO @ Fri, 05 Jul 2019 21:22:25: 9000000 INFO @ Fri, 05 Jul 2019 21:22:25: 9000000 INFO @ Fri, 05 Jul 2019 21:22:29: 9000000 INFO @ Fri, 05 Jul 2019 21:22:32: 10000000 INFO @ Fri, 05 Jul 2019 21:22:33: 10000000 INFO @ Fri, 05 Jul 2019 21:22:36: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:36: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:36: #1 total tags in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:37: #1 tags after filtering in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:37: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:37: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:37: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:37: #1 total tags in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:37: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:37: #1 tags after filtering in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:37: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:37: 10000000 INFO @ Fri, 05 Jul 2019 21:22:37: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:22:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:22:41: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:41: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:41: #1 total tags in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:41: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:41: #1 tags after filtering in treatment: 10521931 INFO @ Fri, 05 Jul 2019 21:22:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:41: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:42: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609706/SRX2609706.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。