Job ID = 2010037 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,618,730 reads read : 17,618,730 reads written : 17,618,730 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:38 17618730 reads; of these: 17618730 (100.00%) were unpaired; of these: 850367 (4.83%) aligned 0 times 13172489 (74.76%) aligned exactly 1 time 3595874 (20.41%) aligned >1 times 95.17% overall alignment rate Time searching: 00:03:38 Overall time: 00:03:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7297874 / 16768363 = 0.4352 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:17:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:36: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:36: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:44: 1000000 INFO @ Fri, 05 Jul 2019 21:17:45: 1000000 INFO @ Fri, 05 Jul 2019 21:17:47: 1000000 INFO @ Fri, 05 Jul 2019 21:17:52: 2000000 INFO @ Fri, 05 Jul 2019 21:17:53: 2000000 INFO @ Fri, 05 Jul 2019 21:17:56: 2000000 INFO @ Fri, 05 Jul 2019 21:18:00: 3000000 INFO @ Fri, 05 Jul 2019 21:18:01: 3000000 INFO @ Fri, 05 Jul 2019 21:18:04: 3000000 INFO @ Fri, 05 Jul 2019 21:18:07: 4000000 INFO @ Fri, 05 Jul 2019 21:18:08: 4000000 INFO @ Fri, 05 Jul 2019 21:18:13: 4000000 INFO @ Fri, 05 Jul 2019 21:18:15: 5000000 INFO @ Fri, 05 Jul 2019 21:18:16: 5000000 INFO @ Fri, 05 Jul 2019 21:18:22: 6000000 INFO @ Fri, 05 Jul 2019 21:18:23: 5000000 INFO @ Fri, 05 Jul 2019 21:18:23: 6000000 INFO @ Fri, 05 Jul 2019 21:18:30: 7000000 INFO @ Fri, 05 Jul 2019 21:18:31: 7000000 INFO @ Fri, 05 Jul 2019 21:18:31: 6000000 INFO @ Fri, 05 Jul 2019 21:18:38: 8000000 INFO @ Fri, 05 Jul 2019 21:18:39: 8000000 INFO @ Fri, 05 Jul 2019 21:18:39: 7000000 INFO @ Fri, 05 Jul 2019 21:18:45: 9000000 INFO @ Fri, 05 Jul 2019 21:18:46: 9000000 INFO @ Fri, 05 Jul 2019 21:18:47: 8000000 INFO @ Fri, 05 Jul 2019 21:18:49: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:49: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:49: #1 total tags in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:49: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:49: #1 tags after filtering in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:49: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:49: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:18:50: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:50: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:50: #1 total tags in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:50: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:18:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:18:50: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 21:18:50: #1 tags after filtering in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:50: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:50: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:18:51: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:18:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:18:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:18:55: 9000000 INFO @ Fri, 05 Jul 2019 21:18:59: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:59: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:59: #1 total tags in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:59: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:59: #1 tags after filtering in treatment: 9470489 INFO @ Fri, 05 Jul 2019 21:18:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:59: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:19:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:19:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:19:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609702/SRX2609702.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。