Job ID = 2010035 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,066,666 reads read : 16,066,666 reads written : 16,066,666 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:53 16066666 reads; of these: 16066666 (100.00%) were unpaired; of these: 542213 (3.37%) aligned 0 times 13274053 (82.62%) aligned exactly 1 time 2250400 (14.01%) aligned >1 times 96.63% overall alignment rate Time searching: 00:02:53 Overall time: 00:02:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5114194 / 15524453 = 0.3294 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:14:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:25: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:25: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:32: 1000000 INFO @ Fri, 05 Jul 2019 21:14:33: 1000000 INFO @ Fri, 05 Jul 2019 21:14:33: 1000000 INFO @ Fri, 05 Jul 2019 21:14:40: 2000000 INFO @ Fri, 05 Jul 2019 21:14:41: 2000000 INFO @ Fri, 05 Jul 2019 21:14:41: 2000000 INFO @ Fri, 05 Jul 2019 21:14:47: 3000000 INFO @ Fri, 05 Jul 2019 21:14:48: 3000000 INFO @ Fri, 05 Jul 2019 21:14:50: 3000000 INFO @ Fri, 05 Jul 2019 21:14:53: 4000000 INFO @ Fri, 05 Jul 2019 21:14:56: 4000000 INFO @ Fri, 05 Jul 2019 21:14:58: 4000000 INFO @ Fri, 05 Jul 2019 21:15:00: 5000000 INFO @ Fri, 05 Jul 2019 21:15:03: 5000000 INFO @ Fri, 05 Jul 2019 21:15:06: 5000000 INFO @ Fri, 05 Jul 2019 21:15:07: 6000000 INFO @ Fri, 05 Jul 2019 21:15:10: 6000000 INFO @ Fri, 05 Jul 2019 21:15:14: 7000000 INFO @ Fri, 05 Jul 2019 21:15:14: 6000000 INFO @ Fri, 05 Jul 2019 21:15:18: 7000000 INFO @ Fri, 05 Jul 2019 21:15:21: 8000000 INFO @ Fri, 05 Jul 2019 21:15:23: 7000000 INFO @ Fri, 05 Jul 2019 21:15:26: 8000000 INFO @ Fri, 05 Jul 2019 21:15:28: 9000000 INFO @ Fri, 05 Jul 2019 21:15:31: 8000000 INFO @ Fri, 05 Jul 2019 21:15:34: 9000000 INFO @ Fri, 05 Jul 2019 21:15:35: 10000000 INFO @ Fri, 05 Jul 2019 21:15:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:15:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:15:38: #1 total tags in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:15:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:15:38: #1 tags after filtering in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:15:38: #1 finished! INFO @ Fri, 05 Jul 2019 21:15:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:15:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:15:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:15:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:15:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:15:40: 9000000 INFO @ Fri, 05 Jul 2019 21:15:42: 10000000 INFO @ Fri, 05 Jul 2019 21:15:46: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:15:46: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:15:46: #1 total tags in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:15:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:15:46: #1 tags after filtering in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:15:46: #1 finished! INFO @ Fri, 05 Jul 2019 21:15:46: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:15:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:15:47: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:15:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:15:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:15:49: 10000000 INFO @ Fri, 05 Jul 2019 21:15:52: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:15:52: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:15:52: #1 total tags in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:52: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:15:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:15:52: #1 tags after filtering in treatment: 10410259 INFO @ Fri, 05 Jul 2019 21:15:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:15:52: #1 finished! INFO @ Fri, 05 Jul 2019 21:15:52: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:15:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:15:53: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:15:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:15:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2609700/SRX2609700.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。