Job ID = 2010034 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,854,035 reads read : 37,708,070 reads written : 18,854,035 reads 0-length : 18,854,035 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:11 18854035 reads; of these: 18854035 (100.00%) were unpaired; of these: 430122 (2.28%) aligned 0 times 14693361 (77.93%) aligned exactly 1 time 3730552 (19.79%) aligned >1 times 97.72% overall alignment rate Time searching: 00:03:11 Overall time: 00:03:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7326870 / 18423913 = 0.3977 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 21:15:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:15:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:15:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:15:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:15:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:15:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:15:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:15:10: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:15:10: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:15:16: 1000000 INFO @ Fri, 05 Jul 2019 21:15:18: 1000000 INFO @ Fri, 05 Jul 2019 21:15:18: 1000000 INFO @ Fri, 05 Jul 2019 21:15:23: 2000000 INFO @ Fri, 05 Jul 2019 21:15:25: 2000000 INFO @ Fri, 05 Jul 2019 21:15:27: 2000000 INFO @ Fri, 05 Jul 2019 21:15:30: 3000000 INFO @ Fri, 05 Jul 2019 21:15:33: 3000000 INFO @ Fri, 05 Jul 2019 21:15:36: 3000000 INFO @ Fri, 05 Jul 2019 21:15:37: 4000000 INFO @ Fri, 05 Jul 2019 21:15:40: 4000000 INFO @ Fri, 05 Jul 2019 21:15:43: 5000000 INFO @ Fri, 05 Jul 2019 21:15:45: 4000000 INFO @ Fri, 05 Jul 2019 21:15:48: 5000000 INFO @ Fri, 05 Jul 2019 21:15:50: 6000000 INFO @ Fri, 05 Jul 2019 21:15:54: 5000000 INFO @ Fri, 05 Jul 2019 21:15:55: 6000000 INFO @ Fri, 05 Jul 2019 21:15:57: 7000000 INFO @ Fri, 05 Jul 2019 21:16:02: 7000000 INFO @ Fri, 05 Jul 2019 21:16:02: 6000000 INFO @ Fri, 05 Jul 2019 21:16:04: 8000000 INFO @ Fri, 05 Jul 2019 21:16:10: 8000000 INFO @ Fri, 05 Jul 2019 21:16:11: 9000000 INFO @ Fri, 05 Jul 2019 21:16:12: 7000000 INFO @ Fri, 05 Jul 2019 21:16:17: 10000000 INFO @ Fri, 05 Jul 2019 21:16:18: 9000000 INFO @ Fri, 05 Jul 2019 21:16:20: 8000000 INFO @ Fri, 05 Jul 2019 21:16:24: 11000000 INFO @ Fri, 05 Jul 2019 21:16:25: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:25: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:25: #1 total tags in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:25: 10000000 INFO @ Fri, 05 Jul 2019 21:16:25: #1 tags after filtering in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:25: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:26: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:29: 9000000 INFO @ Fri, 05 Jul 2019 21:16:32: 11000000 INFO @ Fri, 05 Jul 2019 21:16:33: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:33: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:33: #1 total tags in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:33: #1 tags after filtering in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:33: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:34: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:37: 10000000 INFO @ Fri, 05 Jul 2019 21:16:47: 11000000 INFO @ Fri, 05 Jul 2019 21:16:48: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:48: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:48: #1 total tags in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:48: #1 tags after filtering in treatment: 11097043 INFO @ Fri, 05 Jul 2019 21:16:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:48: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249064/SRX249064.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。