Job ID = 2010032 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,821,504 reads read : 47,643,008 reads written : 23,821,504 reads 0-length : 23,821,504 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:07 23821504 reads; of these: 23821504 (100.00%) were unpaired; of these: 662679 (2.78%) aligned 0 times 18717325 (78.57%) aligned exactly 1 time 4441500 (18.64%) aligned >1 times 97.22% overall alignment rate Time searching: 00:04:08 Overall time: 00:04:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10171930 / 23158825 = 0.4392 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:34:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:34:06: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:34:06: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:34:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:34:07: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:34:07: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:34:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:34:08: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:34:08: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:34:14: 1000000 INFO @ Fri, 05 Jul 2019 21:34:17: 1000000 INFO @ Fri, 05 Jul 2019 21:34:18: 1000000 INFO @ Fri, 05 Jul 2019 21:34:21: 2000000 INFO @ Fri, 05 Jul 2019 21:34:27: 2000000 INFO @ Fri, 05 Jul 2019 21:34:27: 2000000 INFO @ Fri, 05 Jul 2019 21:34:28: 3000000 INFO @ Fri, 05 Jul 2019 21:34:35: 4000000 INFO @ Fri, 05 Jul 2019 21:34:36: 3000000 INFO @ Fri, 05 Jul 2019 21:34:36: 3000000 INFO @ Fri, 05 Jul 2019 21:34:42: 5000000 INFO @ Fri, 05 Jul 2019 21:34:45: 4000000 INFO @ Fri, 05 Jul 2019 21:34:46: 4000000 INFO @ Fri, 05 Jul 2019 21:34:49: 6000000 INFO @ Fri, 05 Jul 2019 21:34:54: 5000000 INFO @ Fri, 05 Jul 2019 21:34:55: 5000000 INFO @ Fri, 05 Jul 2019 21:34:55: 7000000 INFO @ Fri, 05 Jul 2019 21:35:02: 8000000 INFO @ Fri, 05 Jul 2019 21:35:03: 6000000 INFO @ Fri, 05 Jul 2019 21:35:05: 6000000 INFO @ Fri, 05 Jul 2019 21:35:09: 9000000 INFO @ Fri, 05 Jul 2019 21:35:12: 7000000 INFO @ Fri, 05 Jul 2019 21:35:14: 7000000 INFO @ Fri, 05 Jul 2019 21:35:16: 10000000 INFO @ Fri, 05 Jul 2019 21:35:21: 8000000 INFO @ Fri, 05 Jul 2019 21:35:23: 11000000 INFO @ Fri, 05 Jul 2019 21:35:23: 8000000 INFO @ Fri, 05 Jul 2019 21:35:30: 9000000 INFO @ Fri, 05 Jul 2019 21:35:30: 12000000 INFO @ Fri, 05 Jul 2019 21:35:33: 9000000 INFO @ Fri, 05 Jul 2019 21:35:36: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:35:36: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:35:36: #1 total tags in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:35:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:35:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:35:37: #1 tags after filtering in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:35:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:35:37: #1 finished! INFO @ Fri, 05 Jul 2019 21:35:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:35:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:35:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:35:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:35:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:35:38: 10000000 INFO @ Fri, 05 Jul 2019 21:35:42: 10000000 INFO @ Fri, 05 Jul 2019 21:35:47: 11000000 INFO @ Fri, 05 Jul 2019 21:35:51: 11000000 INFO @ Fri, 05 Jul 2019 21:35:56: 12000000 INFO @ Fri, 05 Jul 2019 21:36:01: 12000000 INFO @ Fri, 05 Jul 2019 21:36:06: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:36:06: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:36:06: #1 total tags in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:36:06: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:36:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:36:06: #1 tags after filtering in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:36:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:36:06: #1 finished! INFO @ Fri, 05 Jul 2019 21:36:06: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:36:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:36:07: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:36:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:36:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 21:36:09: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:36:09: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:36:09: #1 total tags in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:36:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:36:10: #1 tags after filtering in treatment: 12986895 INFO @ Fri, 05 Jul 2019 21:36:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:36:10: #1 finished! INFO @ Fri, 05 Jul 2019 21:36:10: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:36:11: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:36:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:36:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249062/SRX249062.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。