Job ID = 2010031 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,087,002 reads read : 44,174,004 reads written : 22,087,002 reads 0-length : 22,087,002 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:59 22087002 reads; of these: 22087002 (100.00%) were unpaired; of these: 618221 (2.80%) aligned 0 times 16766093 (75.91%) aligned exactly 1 time 4702688 (21.29%) aligned >1 times 97.20% overall alignment rate Time searching: 00:03:59 Overall time: 00:03:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9377415 / 21468781 = 0.4368 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:17:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:17:10: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:17:10: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:17:15: 1000000 INFO @ Fri, 05 Jul 2019 21:17:17: 1000000 INFO @ Fri, 05 Jul 2019 21:17:18: 1000000 INFO @ Fri, 05 Jul 2019 21:17:22: 2000000 INFO @ Fri, 05 Jul 2019 21:17:25: 2000000 INFO @ Fri, 05 Jul 2019 21:17:25: 2000000 INFO @ Fri, 05 Jul 2019 21:17:28: 3000000 INFO @ Fri, 05 Jul 2019 21:17:32: 3000000 INFO @ Fri, 05 Jul 2019 21:17:32: 3000000 INFO @ Fri, 05 Jul 2019 21:17:34: 4000000 INFO @ Fri, 05 Jul 2019 21:17:39: 4000000 INFO @ Fri, 05 Jul 2019 21:17:39: 4000000 INFO @ Fri, 05 Jul 2019 21:17:41: 5000000 INFO @ Fri, 05 Jul 2019 21:17:46: 5000000 INFO @ Fri, 05 Jul 2019 21:17:46: 5000000 INFO @ Fri, 05 Jul 2019 21:17:47: 6000000 INFO @ Fri, 05 Jul 2019 21:17:53: 6000000 INFO @ Fri, 05 Jul 2019 21:17:53: 6000000 INFO @ Fri, 05 Jul 2019 21:17:54: 7000000 INFO @ Fri, 05 Jul 2019 21:18:00: 7000000 INFO @ Fri, 05 Jul 2019 21:18:00: 7000000 INFO @ Fri, 05 Jul 2019 21:18:00: 8000000 INFO @ Fri, 05 Jul 2019 21:18:06: 9000000 INFO @ Fri, 05 Jul 2019 21:18:07: 8000000 INFO @ Fri, 05 Jul 2019 21:18:07: 8000000 INFO @ Fri, 05 Jul 2019 21:18:13: 10000000 INFO @ Fri, 05 Jul 2019 21:18:14: 9000000 INFO @ Fri, 05 Jul 2019 21:18:14: 9000000 INFO @ Fri, 05 Jul 2019 21:18:19: 11000000 INFO @ Fri, 05 Jul 2019 21:18:21: 10000000 INFO @ Fri, 05 Jul 2019 21:18:21: 10000000 INFO @ Fri, 05 Jul 2019 21:18:25: 12000000 INFO @ Fri, 05 Jul 2019 21:18:26: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:26: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:26: #1 total tags in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:26: #1 tags after filtering in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:26: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:18:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:18:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:18:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:18:27: 11000000 INFO @ Fri, 05 Jul 2019 21:18:28: 11000000 INFO @ Fri, 05 Jul 2019 21:18:34: 12000000 INFO @ Fri, 05 Jul 2019 21:18:34: 12000000 INFO @ Fri, 05 Jul 2019 21:18:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:35: #1 total tags in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:18:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:18:35: #1 total tags in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:18:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:18:35: #1 tags after filtering in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:35: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:18:35: #1 tags after filtering in treatment: 12091366 INFO @ Fri, 05 Jul 2019 21:18:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:18:35: #1 finished! INFO @ Fri, 05 Jul 2019 21:18:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:18:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:18:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:18:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:18:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:18:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:18:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:18:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249061/SRX249061.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。