Job ID = 2010029 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,316,936 reads read : 40,633,872 reads written : 20,316,936 reads 0-length : 20,316,936 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:19 20316936 reads; of these: 20316936 (100.00%) were unpaired; of these: 552843 (2.72%) aligned 0 times 15078169 (74.21%) aligned exactly 1 time 4685924 (23.06%) aligned >1 times 97.28% overall alignment rate Time searching: 00:03:19 Overall time: 00:03:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8466536 / 19764093 = 0.4284 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:14:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:48: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:48: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:50: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:50: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:56: 1000000 INFO @ Fri, 05 Jul 2019 21:14:57: 1000000 INFO @ Fri, 05 Jul 2019 21:14:58: 1000000 INFO @ Fri, 05 Jul 2019 21:15:04: 2000000 INFO @ Fri, 05 Jul 2019 21:15:05: 2000000 INFO @ Fri, 05 Jul 2019 21:15:06: 2000000 INFO @ Fri, 05 Jul 2019 21:15:12: 3000000 INFO @ Fri, 05 Jul 2019 21:15:13: 3000000 INFO @ Fri, 05 Jul 2019 21:15:14: 3000000 INFO @ Fri, 05 Jul 2019 21:15:20: 4000000 INFO @ Fri, 05 Jul 2019 21:15:20: 4000000 INFO @ Fri, 05 Jul 2019 21:15:22: 4000000 INFO @ Fri, 05 Jul 2019 21:15:28: 5000000 INFO @ Fri, 05 Jul 2019 21:15:28: 5000000 INFO @ Fri, 05 Jul 2019 21:15:30: 5000000 INFO @ Fri, 05 Jul 2019 21:15:35: 6000000 INFO @ Fri, 05 Jul 2019 21:15:36: 6000000 INFO @ Fri, 05 Jul 2019 21:15:38: 6000000 INFO @ Fri, 05 Jul 2019 21:15:43: 7000000 INFO @ Fri, 05 Jul 2019 21:15:44: 7000000 INFO @ Fri, 05 Jul 2019 21:15:46: 7000000 INFO @ Fri, 05 Jul 2019 21:15:50: 8000000 INFO @ Fri, 05 Jul 2019 21:15:52: 8000000 INFO @ Fri, 05 Jul 2019 21:15:54: 8000000 INFO @ Fri, 05 Jul 2019 21:15:57: 9000000 INFO @ Fri, 05 Jul 2019 21:16:00: 9000000 INFO @ Fri, 05 Jul 2019 21:16:02: 9000000 INFO @ Fri, 05 Jul 2019 21:16:05: 10000000 INFO @ Fri, 05 Jul 2019 21:16:08: 10000000 INFO @ Fri, 05 Jul 2019 21:16:09: 10000000 INFO @ Fri, 05 Jul 2019 21:16:12: 11000000 INFO @ Fri, 05 Jul 2019 21:16:14: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:14: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:14: #1 total tags in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:14: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:14: #1 tags after filtering in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:14: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:14: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:15: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:16: 11000000 INFO @ Fri, 05 Jul 2019 21:16:17: 11000000 INFO @ Fri, 05 Jul 2019 21:16:18: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:18: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:18: #1 total tags in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:18: #1 tags after filtering in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:18: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:19: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:16:20: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:16:20: #1 total tags in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:20: #1 tags after filtering in treatment: 11297557 INFO @ Fri, 05 Jul 2019 21:16:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:16:20: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249060/SRX249060.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。