Job ID = 2010028 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,050,816 reads read : 42,101,632 reads written : 21,050,816 reads 0-length : 21,050,816 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:01 21050816 reads; of these: 21050816 (100.00%) were unpaired; of these: 835928 (3.97%) aligned 0 times 15576910 (74.00%) aligned exactly 1 time 4637978 (22.03%) aligned >1 times 96.03% overall alignment rate Time searching: 00:05:01 Overall time: 00:05:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8688681 / 20214888 = 0.4298 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:20:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:20:58: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:20:58: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:20:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:20:59: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:20:59: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:21:00: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:21:00: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:21:07: 1000000 INFO @ Fri, 05 Jul 2019 21:21:09: 1000000 INFO @ Fri, 05 Jul 2019 21:21:10: 1000000 INFO @ Fri, 05 Jul 2019 21:21:19: 2000000 INFO @ Fri, 05 Jul 2019 21:21:20: 2000000 INFO @ Fri, 05 Jul 2019 21:21:20: 2000000 INFO @ Fri, 05 Jul 2019 21:21:29: 3000000 INFO @ Fri, 05 Jul 2019 21:21:30: 3000000 INFO @ Fri, 05 Jul 2019 21:21:31: 3000000 INFO @ Fri, 05 Jul 2019 21:21:39: 4000000 INFO @ Fri, 05 Jul 2019 21:21:40: 4000000 INFO @ Fri, 05 Jul 2019 21:21:42: 4000000 INFO @ Fri, 05 Jul 2019 21:21:49: 5000000 INFO @ Fri, 05 Jul 2019 21:21:51: 5000000 INFO @ Fri, 05 Jul 2019 21:21:52: 5000000 INFO @ Fri, 05 Jul 2019 21:21:58: 6000000 INFO @ Fri, 05 Jul 2019 21:22:02: 6000000 INFO @ Fri, 05 Jul 2019 21:22:03: 6000000 INFO @ Fri, 05 Jul 2019 21:22:08: 7000000 INFO @ Fri, 05 Jul 2019 21:22:13: 7000000 INFO @ Fri, 05 Jul 2019 21:22:14: 7000000 INFO @ Fri, 05 Jul 2019 21:22:17: 8000000 INFO @ Fri, 05 Jul 2019 21:22:24: 8000000 INFO @ Fri, 05 Jul 2019 21:22:25: 8000000 INFO @ Fri, 05 Jul 2019 21:22:27: 9000000 INFO @ Fri, 05 Jul 2019 21:22:34: 9000000 INFO @ Fri, 05 Jul 2019 21:22:36: 9000000 INFO @ Fri, 05 Jul 2019 21:22:37: 10000000 INFO @ Fri, 05 Jul 2019 21:22:45: 10000000 INFO @ Fri, 05 Jul 2019 21:22:46: 11000000 INFO @ Fri, 05 Jul 2019 21:22:46: 10000000 INFO @ Fri, 05 Jul 2019 21:22:51: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:22:51: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:22:51: #1 total tags in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:22:51: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:22:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:22:51: #1 tags after filtering in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:22:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:22:51: #1 finished! INFO @ Fri, 05 Jul 2019 21:22:51: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:22:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:22:52: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:22:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:22:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:22:56: 11000000 INFO @ Fri, 05 Jul 2019 21:22:57: 11000000 INFO @ Fri, 05 Jul 2019 21:23:01: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:23:01: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:23:01: #1 total tags in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:23:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:23:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:23:02: #1 tags after filtering in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:23:02: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:23:02: #1 finished! INFO @ Fri, 05 Jul 2019 21:23:02: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:23:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:23:02: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:23:02: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:23:02: #1 total tags in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:23:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:23:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:23:03: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:23:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:23:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:23:03: #1 tags after filtering in treatment: 11526207 INFO @ Fri, 05 Jul 2019 21:23:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:23:03: #1 finished! INFO @ Fri, 05 Jul 2019 21:23:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:23:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:23:04: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:23:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:23:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX249059/SRX249059.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。