Job ID = 10608909 sra ファイルのダウンロード中... Completed: 1934868K bytes transferred in 27 seconds (583008K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Written 35737012 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2469160/SRR5151070.sra Written 35737012 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:38:09 35737012 reads; of these: 35737012 (100.00%) were paired; of these: 1416050 (3.96%) aligned concordantly 0 times 25394824 (71.06%) aligned concordantly exactly 1 time 8926138 (24.98%) aligned concordantly >1 times ---- 1416050 pairs aligned concordantly 0 times; of these: 145384 (10.27%) aligned discordantly 1 time ---- 1270666 pairs aligned 0 times concordantly or discordantly; of these: 2541332 mates make up the pairs; of these: 2075155 (81.66%) aligned 0 times 211698 (8.33%) aligned exactly 1 time 254479 (10.01%) aligned >1 times 97.10% overall alignment rate Time searching: 00:38:09 Overall time: 00:38:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 32 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 8317401 / 34426777 = 0.2416 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:37:39: # Command line: callpeak -t SRX2469160.bam -f BAM -g 12100000 -n SRX2469160.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2469160.10 # format = BAM # ChIP-seq file = ['SRX2469160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:37:39: #1 read tag files... INFO @ Thu, 03 May 2018 23:37:39: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:37:40: # Command line: callpeak -t SRX2469160.bam -f BAM -g 12100000 -n SRX2469160.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2469160.20 # format = BAM # ChIP-seq file = ['SRX2469160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:37:40: #1 read tag files... INFO @ Thu, 03 May 2018 23:37:40: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:37:40: # Command line: callpeak -t SRX2469160.bam -f BAM -g 12100000 -n SRX2469160.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2469160.05 # format = BAM # ChIP-seq file = ['SRX2469160.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:37:40: #1 read tag files... INFO @ Thu, 03 May 2018 23:37:40: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:37:46: 1000000 INFO @ Thu, 03 May 2018 23:37:46: 1000000 INFO @ Thu, 03 May 2018 23:37:46: 1000000 INFO @ Thu, 03 May 2018 23:37:53: 2000000 INFO @ Thu, 03 May 2018 23:37:53: 2000000 INFO @ Thu, 03 May 2018 23:37:53: 2000000 INFO @ Thu, 03 May 2018 23:37:59: 3000000 INFO @ Thu, 03 May 2018 23:38:00: 3000000 INFO @ Thu, 03 May 2018 23:38:00: 3000000 INFO @ Thu, 03 May 2018 23:38:06: 4000000 INFO @ Thu, 03 May 2018 23:38:07: 4000000 INFO @ Thu, 03 May 2018 23:38:07: 4000000 INFO @ Thu, 03 May 2018 23:38:12: 5000000 INFO @ Thu, 03 May 2018 23:38:13: 5000000 INFO @ Thu, 03 May 2018 23:38:13: 5000000 INFO @ Thu, 03 May 2018 23:38:18: 6000000 INFO @ Thu, 03 May 2018 23:38:20: 6000000 INFO @ Thu, 03 May 2018 23:38:20: 6000000 INFO @ Thu, 03 May 2018 23:38:25: 7000000 INFO @ Thu, 03 May 2018 23:38:27: 7000000 INFO @ Thu, 03 May 2018 23:38:27: 7000000 INFO @ Thu, 03 May 2018 23:38:31: 8000000 INFO @ Thu, 03 May 2018 23:38:34: 8000000 INFO @ Thu, 03 May 2018 23:38:34: 8000000 INFO @ Thu, 03 May 2018 23:38:38: 9000000 INFO @ Thu, 03 May 2018 23:38:40: 9000000 INFO @ Thu, 03 May 2018 23:38:40: 9000000 INFO @ Thu, 03 May 2018 23:38:44: 10000000 INFO @ Thu, 03 May 2018 23:38:47: 10000000 INFO @ Thu, 03 May 2018 23:38:47: 10000000 INFO @ Thu, 03 May 2018 23:38:51: 11000000 INFO @ Thu, 03 May 2018 23:38:54: 11000000 INFO @ Thu, 03 May 2018 23:38:54: 11000000 INFO @ Thu, 03 May 2018 23:38:57: 12000000 INFO @ Thu, 03 May 2018 23:39:01: 12000000 INFO @ Thu, 03 May 2018 23:39:01: 12000000 INFO @ Thu, 03 May 2018 23:39:04: 13000000 INFO @ Thu, 03 May 2018 23:39:07: 13000000 INFO @ Thu, 03 May 2018 23:39:07: 13000000 INFO @ Thu, 03 May 2018 23:39:10: 14000000 INFO @ Thu, 03 May 2018 23:39:14: 14000000 INFO @ Thu, 03 May 2018 23:39:14: 14000000 INFO @ Thu, 03 May 2018 23:39:16: 15000000 INFO @ Thu, 03 May 2018 23:39:21: 15000000 INFO @ Thu, 03 May 2018 23:39:21: 15000000 INFO @ Thu, 03 May 2018 23:39:23: 16000000 INFO @ Thu, 03 May 2018 23:39:27: 16000000 INFO @ Thu, 03 May 2018 23:39:27: 16000000 INFO @ Thu, 03 May 2018 23:39:29: 17000000 INFO @ Thu, 03 May 2018 23:39:34: 17000000 INFO @ Thu, 03 May 2018 23:39:34: 17000000 INFO @ Thu, 03 May 2018 23:39:36: 18000000 INFO @ Thu, 03 May 2018 23:39:41: 18000000 INFO @ Thu, 03 May 2018 23:39:41: 18000000 INFO @ Thu, 03 May 2018 23:39:42: 19000000 INFO @ Thu, 03 May 2018 23:39:47: 19000000 INFO @ Thu, 03 May 2018 23:39:47: 19000000 INFO @ Thu, 03 May 2018 23:39:49: 20000000 INFO @ Thu, 03 May 2018 23:39:54: 20000000 INFO @ Thu, 03 May 2018 23:39:54: 20000000 INFO @ Thu, 03 May 2018 23:39:55: 21000000 INFO @ Thu, 03 May 2018 23:40:01: 21000000 INFO @ Thu, 03 May 2018 23:40:01: 21000000 INFO @ Thu, 03 May 2018 23:40:01: 22000000 INFO @ Thu, 03 May 2018 23:40:07: 22000000 INFO @ Thu, 03 May 2018 23:40:07: 22000000 INFO @ Thu, 03 May 2018 23:40:08: 23000000 INFO @ Thu, 03 May 2018 23:40:14: 23000000 INFO @ Thu, 03 May 2018 23:40:14: 23000000 INFO @ Thu, 03 May 2018 23:40:14: 24000000 INFO @ Thu, 03 May 2018 23:40:21: 24000000 INFO @ Thu, 03 May 2018 23:40:21: 24000000 INFO @ Thu, 03 May 2018 23:40:21: 25000000 INFO @ Thu, 03 May 2018 23:40:27: 25000000 INFO @ Thu, 03 May 2018 23:40:27: 25000000 INFO @ Thu, 03 May 2018 23:40:27: 26000000 INFO @ Thu, 03 May 2018 23:40:34: 26000000 INFO @ Thu, 03 May 2018 23:40:34: 26000000 INFO @ Thu, 03 May 2018 23:40:34: 27000000 INFO @ Thu, 03 May 2018 23:40:41: 27000000 INFO @ Thu, 03 May 2018 23:40:41: 27000000 INFO @ Thu, 03 May 2018 23:40:41: 28000000 INFO @ Thu, 03 May 2018 23:40:47: 28000000 INFO @ Thu, 03 May 2018 23:40:48: 28000000 INFO @ Thu, 03 May 2018 23:40:48: 29000000 INFO @ Thu, 03 May 2018 23:40:54: 29000000 INFO @ Thu, 03 May 2018 23:40:55: 29000000 INFO @ Thu, 03 May 2018 23:40:55: 30000000 INFO @ Thu, 03 May 2018 23:41:00: 30000000 INFO @ Thu, 03 May 2018 23:41:01: 30000000 INFO @ Thu, 03 May 2018 23:41:02: 31000000 INFO @ Thu, 03 May 2018 23:41:06: 31000000 INFO @ Thu, 03 May 2018 23:41:08: 31000000 INFO @ Thu, 03 May 2018 23:41:08: 32000000 INFO @ Thu, 03 May 2018 23:41:13: 32000000 INFO @ Thu, 03 May 2018 23:41:15: 32000000 INFO @ Thu, 03 May 2018 23:41:15: 33000000 INFO @ Thu, 03 May 2018 23:41:19: 33000000 INFO @ Thu, 03 May 2018 23:41:22: 33000000 INFO @ Thu, 03 May 2018 23:41:22: 34000000 INFO @ Thu, 03 May 2018 23:41:25: 34000000 INFO @ Thu, 03 May 2018 23:41:28: 34000000 INFO @ Thu, 03 May 2018 23:41:28: 35000000 INFO @ Thu, 03 May 2018 23:41:32: 35000000 INFO @ Thu, 03 May 2018 23:41:35: 35000000 INFO @ Thu, 03 May 2018 23:41:35: 36000000 INFO @ Thu, 03 May 2018 23:41:38: 36000000 INFO @ Thu, 03 May 2018 23:41:42: 36000000 INFO @ Thu, 03 May 2018 23:41:42: 37000000 INFO @ Thu, 03 May 2018 23:41:45: 37000000 INFO @ Thu, 03 May 2018 23:41:48: 37000000 INFO @ Thu, 03 May 2018 23:41:49: 38000000 INFO @ Thu, 03 May 2018 23:41:51: 38000000 INFO @ Thu, 03 May 2018 23:41:55: 38000000 INFO @ Thu, 03 May 2018 23:41:55: 39000000 INFO @ Thu, 03 May 2018 23:41:57: 39000000 INFO @ Thu, 03 May 2018 23:42:02: 39000000 INFO @ Thu, 03 May 2018 23:42:02: 40000000 INFO @ Thu, 03 May 2018 23:42:04: 40000000 INFO @ Thu, 03 May 2018 23:42:08: 40000000 INFO @ Thu, 03 May 2018 23:42:09: 41000000 INFO @ Thu, 03 May 2018 23:42:10: 41000000 INFO @ Thu, 03 May 2018 23:42:15: 41000000 INFO @ Thu, 03 May 2018 23:42:16: 42000000 INFO @ Thu, 03 May 2018 23:42:17: 42000000 INFO @ Thu, 03 May 2018 23:42:22: 42000000 INFO @ Thu, 03 May 2018 23:42:23: 43000000 INFO @ Thu, 03 May 2018 23:42:23: 43000000 INFO @ Thu, 03 May 2018 23:42:29: 43000000 INFO @ Thu, 03 May 2018 23:42:30: 44000000 INFO @ Thu, 03 May 2018 23:42:30: 44000000 INFO @ Thu, 03 May 2018 23:42:36: 44000000 INFO @ Thu, 03 May 2018 23:42:36: 45000000 INFO @ Thu, 03 May 2018 23:42:37: 45000000 INFO @ Thu, 03 May 2018 23:42:43: 45000000 INFO @ Thu, 03 May 2018 23:42:43: 46000000 INFO @ Thu, 03 May 2018 23:42:44: 46000000 INFO @ Thu, 03 May 2018 23:42:49: 46000000 INFO @ Thu, 03 May 2018 23:42:50: 47000000 INFO @ Thu, 03 May 2018 23:42:51: 47000000 INFO @ Thu, 03 May 2018 23:42:56: 47000000 INFO @ Thu, 03 May 2018 23:42:57: 48000000 INFO @ Thu, 03 May 2018 23:42:58: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 03 May 2018 23:43:02: 48000000 INFO @ Thu, 03 May 2018 23:43:04: 49000000 INFO @ Thu, 03 May 2018 23:43:05: 49000000 INFO @ Thu, 03 May 2018 23:43:08: 49000000 INFO @ Thu, 03 May 2018 23:43:11: 50000000 INFO @ Thu, 03 May 2018 23:43:12: 50000000 INFO @ Thu, 03 May 2018 23:43:15: 50000000 INFO @ Thu, 03 May 2018 23:43:18: 51000000 INFO @ Thu, 03 May 2018 23:43:19: 51000000 BigWig に変換しました。 INFO @ Thu, 03 May 2018 23:43:21: 51000000 INFO @ Thu, 03 May 2018 23:43:25: 52000000 INFO @ Thu, 03 May 2018 23:43:26: 52000000 INFO @ Thu, 03 May 2018 23:43:28: 52000000 INFO @ Thu, 03 May 2018 23:43:31: #1 tag size is determined as 75 bps INFO @ Thu, 03 May 2018 23:43:31: #1 tag size = 75 INFO @ Thu, 03 May 2018 23:43:31: #1 total tags in treatment: 26012829 INFO @ Thu, 03 May 2018 23:43:31: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:43:31: #1 tags after filtering in treatment: 14340792 INFO @ Thu, 03 May 2018 23:43:31: #1 Redundant rate of treatment: 0.45 INFO @ Thu, 03 May 2018 23:43:31: #1 finished! INFO @ Thu, 03 May 2018 23:43:31: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:43:31: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:43:32: #1 tag size is determined as 75 bps INFO @ Thu, 03 May 2018 23:43:32: #1 tag size = 75 INFO @ Thu, 03 May 2018 23:43:32: #1 total tags in treatment: 26012829 INFO @ Thu, 03 May 2018 23:43:32: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:43:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:43:32: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:43:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:43:32: Process for pairing-model is terminated! cat: SRX2469160.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2469160.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:43:32: #1 tags after filtering in treatment: 14340792 INFO @ Thu, 03 May 2018 23:43:32: #1 Redundant rate of treatment: 0.45 INFO @ Thu, 03 May 2018 23:43:32: #1 finished! INFO @ Thu, 03 May 2018 23:43:32: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:43:32: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:43:33: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:43:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:43:33: Process for pairing-model is terminated! cat: SRX2469160.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2469160.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:43:33: #1 tag size is determined as 75 bps INFO @ Thu, 03 May 2018 23:43:33: #1 tag size = 75 INFO @ Thu, 03 May 2018 23:43:33: #1 total tags in treatment: 26012829 INFO @ Thu, 03 May 2018 23:43:33: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:43:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:43:34: #1 tags after filtering in treatment: 14340792 INFO @ Thu, 03 May 2018 23:43:34: #1 Redundant rate of treatment: 0.45 INFO @ Thu, 03 May 2018 23:43:34: #1 finished! INFO @ Thu, 03 May 2018 23:43:34: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:43:34: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:43:35: #2 number of paired peaks: 0 WARNING @ Thu, 03 May 2018 23:43:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:43:35: Process for pairing-model is terminated! cat: SRX2469160.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2469160.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2469160.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling