Job ID = 2640810 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 19,653,720 reads read : 39,307,440 reads written : 19,653,720 reads 0-length : 19,653,720 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:11 19653720 reads; of these: 19653720 (100.00%) were unpaired; of these: 841822 (4.28%) aligned 0 times 16289485 (82.88%) aligned exactly 1 time 2522413 (12.83%) aligned >1 times 95.72% overall alignment rate Time searching: 00:08:11 Overall time: 00:08:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7399045 / 18811898 = 0.3933 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:55:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:55:49: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:55:49: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:55:59: 1000000 INFO @ Sat, 24 Aug 2019 19:56:09: 2000000 INFO @ Sat, 24 Aug 2019 19:56:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:56:19: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:56:19: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:56:19: 3000000 INFO @ Sat, 24 Aug 2019 19:56:28: 4000000 INFO @ Sat, 24 Aug 2019 19:56:29: 1000000 INFO @ Sat, 24 Aug 2019 19:56:38: 5000000 INFO @ Sat, 24 Aug 2019 19:56:39: 2000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:56:48: 6000000 INFO @ Sat, 24 Aug 2019 19:56:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:56:49: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:56:49: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:56:49: 3000000 INFO @ Sat, 24 Aug 2019 19:56:58: 7000000 INFO @ Sat, 24 Aug 2019 19:56:58: 1000000 INFO @ Sat, 24 Aug 2019 19:56:58: 4000000 INFO @ Sat, 24 Aug 2019 19:57:07: 2000000 INFO @ Sat, 24 Aug 2019 19:57:08: 8000000 INFO @ Sat, 24 Aug 2019 19:57:08: 5000000 INFO @ Sat, 24 Aug 2019 19:57:16: 3000000 INFO @ Sat, 24 Aug 2019 19:57:17: 9000000 INFO @ Sat, 24 Aug 2019 19:57:18: 6000000 INFO @ Sat, 24 Aug 2019 19:57:25: 4000000 INFO @ Sat, 24 Aug 2019 19:57:28: 7000000 INFO @ Sat, 24 Aug 2019 19:57:28: 10000000 INFO @ Sat, 24 Aug 2019 19:57:34: 5000000 INFO @ Sat, 24 Aug 2019 19:57:38: 8000000 INFO @ Sat, 24 Aug 2019 19:57:38: 11000000 INFO @ Sat, 24 Aug 2019 19:57:43: #1 tag size is determined as 136 bps INFO @ Sat, 24 Aug 2019 19:57:43: #1 tag size = 136 INFO @ Sat, 24 Aug 2019 19:57:43: #1 total tags in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:57:43: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:57:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:57:43: #1 tags after filtering in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:57:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:57:43: #1 finished! INFO @ Sat, 24 Aug 2019 19:57:43: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:57:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:57:43: 6000000 INFO @ Sat, 24 Aug 2019 19:57:44: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:57:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:57:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:57:47: 9000000 INFO @ Sat, 24 Aug 2019 19:57:52: 7000000 INFO @ Sat, 24 Aug 2019 19:57:57: 10000000 INFO @ Sat, 24 Aug 2019 19:58:01: 8000000 INFO @ Sat, 24 Aug 2019 19:58:07: 11000000 INFO @ Sat, 24 Aug 2019 19:58:11: 9000000 INFO @ Sat, 24 Aug 2019 19:58:11: #1 tag size is determined as 136 bps INFO @ Sat, 24 Aug 2019 19:58:11: #1 tag size = 136 INFO @ Sat, 24 Aug 2019 19:58:11: #1 total tags in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:58:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:58:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:58:12: #1 tags after filtering in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:58:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:58:12: #1 finished! INFO @ Sat, 24 Aug 2019 19:58:12: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:58:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:58:12: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:58:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:58:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:58:20: 10000000 INFO @ Sat, 24 Aug 2019 19:58:29: 11000000 INFO @ Sat, 24 Aug 2019 19:58:33: #1 tag size is determined as 136 bps INFO @ Sat, 24 Aug 2019 19:58:33: #1 tag size = 136 INFO @ Sat, 24 Aug 2019 19:58:33: #1 total tags in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:58:33: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:58:33: #1 tags after filtering in treatment: 11412853 INFO @ Sat, 24 Aug 2019 19:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:58:33: #1 finished! INFO @ Sat, 24 Aug 2019 19:58:33: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:58:34: #2 number of paired peaks: 0 WARNING @ Sat, 24 Aug 2019 19:58:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439297/SRX2439297.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。