Job ID = 2640807 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,388,675 reads read : 22,777,350 reads written : 11,388,675 reads 0-length : 11,388,675 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:52 11388675 reads; of these: 11388675 (100.00%) were unpaired; of these: 648599 (5.70%) aligned 0 times 9710570 (85.27%) aligned exactly 1 time 1029506 (9.04%) aligned >1 times 94.30% overall alignment rate Time searching: 00:04:52 Overall time: 00:04:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6894797 / 10740076 = 0.6420 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 19:36:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:36:45: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:36:45: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:36:57: 1000000 INFO @ Sat, 24 Aug 2019 19:37:09: 2000000 INFO @ Sat, 24 Aug 2019 19:37:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:37:14: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:37:14: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:37:21: 3000000 INFO @ Sat, 24 Aug 2019 19:37:29: 1000000 INFO @ Sat, 24 Aug 2019 19:37:31: #1 tag size is determined as 82 bps INFO @ Sat, 24 Aug 2019 19:37:31: #1 tag size = 82 INFO @ Sat, 24 Aug 2019 19:37:31: #1 total tags in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:37:31: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:37:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:37:31: #1 tags after filtering in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:37:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:37:31: #1 finished! INFO @ Sat, 24 Aug 2019 19:37:31: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:37:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:37:32: #2 number of paired peaks: 26 WARNING @ Sat, 24 Aug 2019 19:37:32: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:37:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... INFO @ Sat, 24 Aug 2019 19:37:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 19:37:44: #1 read tag files... INFO @ Sat, 24 Aug 2019 19:37:44: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 19:37:45: 2000000 INFO @ Sat, 24 Aug 2019 19:37:56: 1000000 INFO @ Sat, 24 Aug 2019 19:38:01: 3000000 INFO @ Sat, 24 Aug 2019 19:38:08: 2000000 INFO @ Sat, 24 Aug 2019 19:38:14: #1 tag size is determined as 82 bps INFO @ Sat, 24 Aug 2019 19:38:14: #1 tag size = 82 INFO @ Sat, 24 Aug 2019 19:38:14: #1 total tags in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:38:14: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:38:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:38:14: #1 tags after filtering in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:38:14: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:38:14: #1 finished! INFO @ Sat, 24 Aug 2019 19:38:14: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:38:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:38:15: #2 number of paired peaks: 26 WARNING @ Sat, 24 Aug 2019 19:38:15: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:38:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 19:38:21: 3000000 INFO @ Sat, 24 Aug 2019 19:38:31: #1 tag size is determined as 82 bps INFO @ Sat, 24 Aug 2019 19:38:31: #1 tag size = 82 INFO @ Sat, 24 Aug 2019 19:38:31: #1 total tags in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:38:31: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 19:38:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 19:38:31: #1 tags after filtering in treatment: 3845279 INFO @ Sat, 24 Aug 2019 19:38:31: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 19:38:31: #1 finished! INFO @ Sat, 24 Aug 2019 19:38:31: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 19:38:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 19:38:31: #2 number of paired peaks: 26 WARNING @ Sat, 24 Aug 2019 19:38:31: Too few paired peaks (26) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 24 Aug 2019 19:38:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2439294/SRX2439294.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。