Job ID = 9159992 sra ファイルのダウンロード中... Completed: 587756K bytes transferred in 9 seconds (510858K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 33673698 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2381671/SRR5061145.sra Written 33673698 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:47 33673698 reads; of these: 33673698 (100.00%) were unpaired; of these: 1678503 (4.98%) aligned 0 times 25645223 (76.16%) aligned exactly 1 time 6349972 (18.86%) aligned >1 times 95.02% overall alignment rate Time searching: 00:05:47 Overall time: 00:05:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21536862 / 31995195 = 0.6731 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:42:04: # Command line: callpeak -t SRX2381671.bam -f BAM -g 12100000 -n SRX2381671.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2381671.10 # format = BAM # ChIP-seq file = ['SRX2381671.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:42:04: # Command line: callpeak -t SRX2381671.bam -f BAM -g 12100000 -n SRX2381671.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2381671.05 # format = BAM # ChIP-seq file = ['SRX2381671.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:42:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:42:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:42:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:42:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:42:04: # Command line: callpeak -t SRX2381671.bam -f BAM -g 12100000 -n SRX2381671.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2381671.20 # format = BAM # ChIP-seq file = ['SRX2381671.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:42:04: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:42:04: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:42:10: 1000000 INFO @ Wed, 28 Jun 2017 01:42:10: 1000000 INFO @ Wed, 28 Jun 2017 01:42:10: 1000000 INFO @ Wed, 28 Jun 2017 01:42:17: 2000000 INFO @ Wed, 28 Jun 2017 01:42:17: 2000000 INFO @ Wed, 28 Jun 2017 01:42:17: 2000000 INFO @ Wed, 28 Jun 2017 01:42:23: 3000000 INFO @ Wed, 28 Jun 2017 01:42:24: 3000000 INFO @ Wed, 28 Jun 2017 01:42:24: 3000000 INFO @ Wed, 28 Jun 2017 01:42:30: 4000000 INFO @ Wed, 28 Jun 2017 01:42:30: 4000000 INFO @ Wed, 28 Jun 2017 01:42:31: 4000000 INFO @ Wed, 28 Jun 2017 01:42:37: 5000000 INFO @ Wed, 28 Jun 2017 01:42:37: 5000000 INFO @ Wed, 28 Jun 2017 01:42:38: 5000000 INFO @ Wed, 28 Jun 2017 01:42:43: 6000000 INFO @ Wed, 28 Jun 2017 01:42:44: 6000000 INFO @ Wed, 28 Jun 2017 01:42:45: 6000000 INFO @ Wed, 28 Jun 2017 01:42:50: 7000000 INFO @ Wed, 28 Jun 2017 01:42:50: 7000000 INFO @ Wed, 28 Jun 2017 01:42:52: 7000000 INFO @ Wed, 28 Jun 2017 01:42:56: 8000000 INFO @ Wed, 28 Jun 2017 01:42:57: 8000000 INFO @ Wed, 28 Jun 2017 01:42:59: 8000000 INFO @ Wed, 28 Jun 2017 01:43:03: 9000000 INFO @ Wed, 28 Jun 2017 01:43:04: 9000000 INFO @ Wed, 28 Jun 2017 01:43:06: 9000000 INFO @ Wed, 28 Jun 2017 01:43:09: 10000000 INFO @ Wed, 28 Jun 2017 01:43:10: 10000000 INFO @ Wed, 28 Jun 2017 01:43:12: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:43:12: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:43:12: #1 total tags in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:12: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:43:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:43:12: #1 tags after filtering in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:12: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:43:12: #1 finished! INFO @ Wed, 28 Jun 2017 01:43:12: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:43:12: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:43:13: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:43:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:43:13: Process for pairing-model is terminated! cat: SRX2381671.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381671.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:43:13: 10000000 INFO @ Wed, 28 Jun 2017 01:43:13: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:43:13: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:43:13: #1 total tags in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:13: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:43:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:43:13: #1 tags after filtering in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:43:13: #1 finished! INFO @ Wed, 28 Jun 2017 01:43:13: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:43:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:43:14: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:43:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:43:14: Process for pairing-model is terminated! cat: SRX2381671.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381671.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:43:16: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:43:16: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:43:16: #1 total tags in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:16: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:43:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:43:16: #1 tags after filtering in treatment: 10458333 INFO @ Wed, 28 Jun 2017 01:43:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:43:16: #1 finished! INFO @ Wed, 28 Jun 2017 01:43:16: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:43:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:43:17: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:43:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:43:17: Process for pairing-model is terminated! cat: SRX2381671.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381671.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381671.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。