Job ID = 9159991 sra ファイルのダウンロード中... Completed: 457174K bytes transferred in 7 seconds (482389K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25984890 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2381670/SRR5061144.sra Written 25984890 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:39 25984890 reads; of these: 25984890 (100.00%) were unpaired; of these: 692839 (2.67%) aligned 0 times 20004573 (76.99%) aligned exactly 1 time 5287478 (20.35%) aligned >1 times 97.33% overall alignment rate Time searching: 00:04:39 Overall time: 00:04:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 16853696 / 25292051 = 0.6664 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 01:39:29: # Command line: callpeak -t SRX2381670.bam -f BAM -g 12100000 -n SRX2381670.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2381670.10 # format = BAM # ChIP-seq file = ['SRX2381670.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:39:29: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:39:29: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:39:29: # Command line: callpeak -t SRX2381670.bam -f BAM -g 12100000 -n SRX2381670.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2381670.05 # format = BAM # ChIP-seq file = ['SRX2381670.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:39:29: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:39:29: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:39:29: # Command line: callpeak -t SRX2381670.bam -f BAM -g 12100000 -n SRX2381670.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2381670.20 # format = BAM # ChIP-seq file = ['SRX2381670.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 01:39:29: #1 read tag files... INFO @ Wed, 28 Jun 2017 01:39:29: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 01:39:36: 1000000 INFO @ Wed, 28 Jun 2017 01:39:37: 1000000 INFO @ Wed, 28 Jun 2017 01:39:37: 1000000 INFO @ Wed, 28 Jun 2017 01:39:43: 2000000 INFO @ Wed, 28 Jun 2017 01:39:44: 2000000 INFO @ Wed, 28 Jun 2017 01:39:44: 2000000 INFO @ Wed, 28 Jun 2017 01:39:50: 3000000 INFO @ Wed, 28 Jun 2017 01:39:51: 3000000 INFO @ Wed, 28 Jun 2017 01:39:52: 3000000 INFO @ Wed, 28 Jun 2017 01:39:58: 4000000 INFO @ Wed, 28 Jun 2017 01:39:58: 4000000 INFO @ Wed, 28 Jun 2017 01:40:00: 4000000 INFO @ Wed, 28 Jun 2017 01:40:05: 5000000 INFO @ Wed, 28 Jun 2017 01:40:05: 5000000 INFO @ Wed, 28 Jun 2017 01:40:07: 5000000 INFO @ Wed, 28 Jun 2017 01:40:12: 6000000 INFO @ Wed, 28 Jun 2017 01:40:12: 6000000 INFO @ Wed, 28 Jun 2017 01:40:14: 6000000 INFO @ Wed, 28 Jun 2017 01:40:19: 7000000 INFO @ Wed, 28 Jun 2017 01:40:19: 7000000 INFO @ Wed, 28 Jun 2017 01:40:21: 7000000 INFO @ Wed, 28 Jun 2017 01:40:26: 8000000 INFO @ Wed, 28 Jun 2017 01:40:26: 8000000 INFO @ Wed, 28 Jun 2017 01:40:28: 8000000 INFO @ Wed, 28 Jun 2017 01:40:29: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:40:29: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:40:29: #1 total tags in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:29: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:40:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:40:29: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:40:29: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:40:29: #1 total tags in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:29: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:40:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:40:29: #1 tags after filtering in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:40:29: #1 finished! INFO @ Wed, 28 Jun 2017 01:40:29: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:40:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:40:29: #1 tags after filtering in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:40:29: #1 finished! INFO @ Wed, 28 Jun 2017 01:40:29: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:40:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:40:30: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:40:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:40:30: Process for pairing-model is terminated! cat: SRX2381670.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381670.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:40:30: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:40:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:40:30: Process for pairing-model is terminated! cat: SRX2381670.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381670.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 01:40:31: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 01:40:31: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 01:40:31: #1 total tags in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 01:40:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 01:40:31: #1 tags after filtering in treatment: 8438355 INFO @ Wed, 28 Jun 2017 01:40:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 01:40:31: #1 finished! INFO @ Wed, 28 Jun 2017 01:40:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 01:40:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 01:40:32: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 01:40:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 01:40:32: Process for pairing-model is terminated! cat: SRX2381670.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2381670.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2381670.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。