Job ID = 2010013 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 14,702,488 reads read : 29,404,976 reads written : 29,404,976 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:34 14702488 reads; of these: 14702488 (100.00%) were paired; of these: 3095810 (21.06%) aligned concordantly 0 times 9516917 (64.73%) aligned concordantly exactly 1 time 2089761 (14.21%) aligned concordantly >1 times ---- 3095810 pairs aligned concordantly 0 times; of these: 162655 (5.25%) aligned discordantly 1 time ---- 2933155 pairs aligned 0 times concordantly or discordantly; of these: 5866310 mates make up the pairs; of these: 5386350 (91.82%) aligned 0 times 324680 (5.53%) aligned exactly 1 time 155280 (2.65%) aligned >1 times 81.68% overall alignment rate Time searching: 00:06:34 Overall time: 00:06:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2082345 / 11764628 = 0.1770 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:14:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:14:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:14:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:14:21: 1000000 INFO @ Fri, 05 Jul 2019 21:14:22: 1000000 INFO @ Fri, 05 Jul 2019 21:14:23: 1000000 INFO @ Fri, 05 Jul 2019 21:14:27: 2000000 INFO @ Fri, 05 Jul 2019 21:14:28: 2000000 INFO @ Fri, 05 Jul 2019 21:14:29: 2000000 INFO @ Fri, 05 Jul 2019 21:14:34: 3000000 INFO @ Fri, 05 Jul 2019 21:14:35: 3000000 INFO @ Fri, 05 Jul 2019 21:14:36: 3000000 INFO @ Fri, 05 Jul 2019 21:14:40: 4000000 INFO @ Fri, 05 Jul 2019 21:14:41: 4000000 INFO @ Fri, 05 Jul 2019 21:14:42: 4000000 INFO @ Fri, 05 Jul 2019 21:14:47: 5000000 INFO @ Fri, 05 Jul 2019 21:14:48: 5000000 INFO @ Fri, 05 Jul 2019 21:14:49: 5000000 INFO @ Fri, 05 Jul 2019 21:14:53: 6000000 INFO @ Fri, 05 Jul 2019 21:14:55: 6000000 INFO @ Fri, 05 Jul 2019 21:14:55: 6000000 INFO @ Fri, 05 Jul 2019 21:15:00: 7000000 INFO @ Fri, 05 Jul 2019 21:15:01: 7000000 INFO @ Fri, 05 Jul 2019 21:15:02: 7000000 INFO @ Fri, 05 Jul 2019 21:15:06: 8000000 INFO @ Fri, 05 Jul 2019 21:15:08: 8000000 INFO @ Fri, 05 Jul 2019 21:15:09: 8000000 INFO @ Fri, 05 Jul 2019 21:15:13: 9000000 INFO @ Fri, 05 Jul 2019 21:15:14: 9000000 INFO @ Fri, 05 Jul 2019 21:15:15: 9000000 INFO @ Fri, 05 Jul 2019 21:15:20: 10000000 INFO @ Fri, 05 Jul 2019 21:15:21: 10000000 INFO @ Fri, 05 Jul 2019 21:15:22: 10000000 INFO @ Fri, 05 Jul 2019 21:15:26: 11000000 INFO @ Fri, 05 Jul 2019 21:15:28: 11000000 INFO @ Fri, 05 Jul 2019 21:15:28: 11000000 INFO @ Fri, 05 Jul 2019 21:15:33: 12000000 INFO @ Fri, 05 Jul 2019 21:15:34: 12000000 INFO @ Fri, 05 Jul 2019 21:15:35: 12000000 INFO @ Fri, 05 Jul 2019 21:15:39: 13000000 INFO @ Fri, 05 Jul 2019 21:15:41: 13000000 INFO @ Fri, 05 Jul 2019 21:15:41: 13000000 INFO @ Fri, 05 Jul 2019 21:15:46: 14000000 INFO @ Fri, 05 Jul 2019 21:15:47: 14000000 INFO @ Fri, 05 Jul 2019 21:15:48: 14000000 INFO @ Fri, 05 Jul 2019 21:15:52: 15000000 INFO @ Fri, 05 Jul 2019 21:15:54: 15000000 INFO @ Fri, 05 Jul 2019 21:15:55: 15000000 INFO @ Fri, 05 Jul 2019 21:15:59: 16000000 INFO @ Fri, 05 Jul 2019 21:16:01: 16000000 INFO @ Fri, 05 Jul 2019 21:16:01: 16000000 INFO @ Fri, 05 Jul 2019 21:16:05: 17000000 INFO @ Fri, 05 Jul 2019 21:16:08: 17000000 INFO @ Fri, 05 Jul 2019 21:16:08: 17000000 INFO @ Fri, 05 Jul 2019 21:16:12: 18000000 INFO @ Fri, 05 Jul 2019 21:16:15: 18000000 INFO @ Fri, 05 Jul 2019 21:16:15: 18000000 INFO @ Fri, 05 Jul 2019 21:16:18: 19000000 INFO @ Fri, 05 Jul 2019 21:16:22: 19000000 INFO @ Fri, 05 Jul 2019 21:16:22: 19000000 INFO @ Fri, 05 Jul 2019 21:16:25: #1 tag size is determined as 24 bps INFO @ Fri, 05 Jul 2019 21:16:25: #1 tag size = 24 INFO @ Fri, 05 Jul 2019 21:16:25: #1 total tags in treatment: 9525360 INFO @ Fri, 05 Jul 2019 21:16:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:25: #1 tags after filtering in treatment: 4791968 INFO @ Fri, 05 Jul 2019 21:16:25: #1 Redundant rate of treatment: 0.50 INFO @ Fri, 05 Jul 2019 21:16:25: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:27: #1 tag size is determined as 24 bps INFO @ Fri, 05 Jul 2019 21:16:27: #1 tag size = 24 INFO @ Fri, 05 Jul 2019 21:16:27: #1 total tags in treatment: 9525360 INFO @ Fri, 05 Jul 2019 21:16:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:28: #1 tags after filtering in treatment: 4791968 INFO @ Fri, 05 Jul 2019 21:16:28: #1 Redundant rate of treatment: 0.50 INFO @ Fri, 05 Jul 2019 21:16:28: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:28: #1 tag size is determined as 24 bps INFO @ Fri, 05 Jul 2019 21:16:28: #1 tag size = 24 INFO @ Fri, 05 Jul 2019 21:16:28: #1 total tags in treatment: 9525360 INFO @ Fri, 05 Jul 2019 21:16:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:16:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:16:28: #1 tags after filtering in treatment: 4791968 INFO @ Fri, 05 Jul 2019 21:16:28: #1 Redundant rate of treatment: 0.50 INFO @ Fri, 05 Jul 2019 21:16:28: #1 finished! INFO @ Fri, 05 Jul 2019 21:16:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:16:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:16:28: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:16:28: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:16:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:16:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX235690/SRX235690.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。