Job ID = 11192870 sra ファイルのダウンロード中... Completed: 607554K bytes transferred in 8 seconds (604377K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 10920731 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2252394/SRR4432885.sra Written 10920731 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2252394/SRR4432885.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:20 10920731 reads; of these: 10920731 (100.00%) were paired; of these: 662802 (6.07%) aligned concordantly 0 times 7805070 (71.47%) aligned concordantly exactly 1 time 2452859 (22.46%) aligned concordantly >1 times ---- 662802 pairs aligned concordantly 0 times; of these: 83478 (12.59%) aligned discordantly 1 time ---- 579324 pairs aligned 0 times concordantly or discordantly; of these: 1158648 mates make up the pairs; of these: 793383 (68.47%) aligned 0 times 225708 (19.48%) aligned exactly 1 time 139557 (12.04%) aligned >1 times 96.37% overall alignment rate Time searching: 00:10:20 Overall time: 00:10:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 10299596 / 10335995 = 0.9965 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:20:13: # Command line: callpeak -t SRX2252394.bam -f BAM -g 12100000 -n SRX2252394.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2252394.05 # format = BAM # ChIP-seq file = ['SRX2252394.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:20:13: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:20:13: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:20:13: # Command line: callpeak -t SRX2252394.bam -f BAM -g 12100000 -n SRX2252394.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2252394.10 # format = BAM # ChIP-seq file = ['SRX2252394.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:20:13: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:20:13: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:20:13: # Command line: callpeak -t SRX2252394.bam -f BAM -g 12100000 -n SRX2252394.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2252394.20 # format = BAM # ChIP-seq file = ['SRX2252394.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:20:13: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:20:13: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size is determined as 32 bps INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size = 32 INFO @ Sat, 15 Sep 2018 10:20:15: #1 total tags in treatment: 39090 INFO @ Sat, 15 Sep 2018 10:20:15: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:20:15: #1 tags after filtering in treatment: 32130 INFO @ Sat, 15 Sep 2018 10:20:15: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 10:20:15: #1 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #2 number of paired peaks: 282 WARNING @ Sat, 15 Sep 2018 10:20:15: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Sat, 15 Sep 2018 10:20:15: start model_add_line... INFO @ Sat, 15 Sep 2018 10:20:15: start X-correlation... INFO @ Sat, 15 Sep 2018 10:20:15: end of X-cor INFO @ Sat, 15 Sep 2018 10:20:15: #2 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #2 predicted fragment length is 168 bps INFO @ Sat, 15 Sep 2018 10:20:15: #2 alternative fragment length(s) may be 99,134,168,209,239,378,542,580 bps INFO @ Sat, 15 Sep 2018 10:20:15: #2.2 Generate R script for model : SRX2252394.05_model.r INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size is determined as 32 bps INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size = 32 INFO @ Sat, 15 Sep 2018 10:20:15: #1 total tags in treatment: 39090 INFO @ Sat, 15 Sep 2018 10:20:15: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size is determined as 32 bps INFO @ Sat, 15 Sep 2018 10:20:15: #1 tags after filtering in treatment: 32130 INFO @ Sat, 15 Sep 2018 10:20:15: #1 tag size = 32 INFO @ Sat, 15 Sep 2018 10:20:15: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 10:20:15: #1 total tags in treatment: 39090 INFO @ Sat, 15 Sep 2018 10:20:15: #1 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:20:15: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:20:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #1 tags after filtering in treatment: 32130 INFO @ Sat, 15 Sep 2018 10:20:15: #1 Redundant rate of treatment: 0.18 INFO @ Sat, 15 Sep 2018 10:20:15: #1 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:20:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #2 number of paired peaks: 282 WARNING @ Sat, 15 Sep 2018 10:20:15: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Sat, 15 Sep 2018 10:20:15: start model_add_line... INFO @ Sat, 15 Sep 2018 10:20:15: start X-correlation... INFO @ Sat, 15 Sep 2018 10:20:15: end of X-cor INFO @ Sat, 15 Sep 2018 10:20:15: #2 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #2 predicted fragment length is 168 bps INFO @ Sat, 15 Sep 2018 10:20:15: #2 number of paired peaks: 282 INFO @ Sat, 15 Sep 2018 10:20:15: #2 alternative fragment length(s) may be 99,134,168,209,239,378,542,580 bps WARNING @ Sat, 15 Sep 2018 10:20:15: Fewer paired peaks (282) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 282 pairs to build model! INFO @ Sat, 15 Sep 2018 10:20:15: #2.2 Generate R script for model : SRX2252394.10_model.r INFO @ Sat, 15 Sep 2018 10:20:15: start model_add_line... INFO @ Sat, 15 Sep 2018 10:20:15: start X-correlation... INFO @ Sat, 15 Sep 2018 10:20:15: end of X-cor INFO @ Sat, 15 Sep 2018 10:20:15: #2 finished! INFO @ Sat, 15 Sep 2018 10:20:15: #2 predicted fragment length is 168 bps INFO @ Sat, 15 Sep 2018 10:20:15: #2 alternative fragment length(s) may be 99,134,168,209,239,378,542,580 bps INFO @ Sat, 15 Sep 2018 10:20:15: #2.2 Generate R script for model : SRX2252394.20_model.r INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:20:15: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write output xls file... SRX2252394.05_peaks.xls INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write peak in narrowPeak format file... SRX2252394.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write summits bed file... SRX2252394.05_summits.bed INFO @ Sat, 15 Sep 2018 10:20:15: Done! INFO @ Sat, 15 Sep 2018 10:20:15: #3 Call peaks for each chromosome... pass1 - making usageList (14 chroms): 0 millis pass2 - checking and writing primary data (53 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write output xls file... SRX2252394.10_peaks.xls INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write peak in narrowPeak format file... SRX2252394.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write summits bed file... SRX2252394.10_summits.bed INFO @ Sat, 15 Sep 2018 10:20:15: Done! INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write output xls file... SRX2252394.20_peaks.xls INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write peak in narrowPeak format file... SRX2252394.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:20:15: #4 Write summits bed file... SRX2252394.20_summits.bed INFO @ Sat, 15 Sep 2018 10:20:15: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (24 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (8 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。