Job ID = 9162398 sra ファイルのダウンロード中... Completed: 3151839K bytes transferred in 29 seconds (883647K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 43440743 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2250627/SRR4429108.sra Written 43440743 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:34 43440743 reads; of these: 43440743 (100.00%) were unpaired; of these: 2557169 (5.89%) aligned 0 times 33479590 (77.07%) aligned exactly 1 time 7403984 (17.04%) aligned >1 times 94.11% overall alignment rate Time searching: 00:13:34 Overall time: 00:13:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 23169120 / 40883574 = 0.5667 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 08:09:20: # Command line: callpeak -t SRX2250627.bam -f BAM -g 12100000 -n SRX2250627.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2250627.10 # format = BAM # ChIP-seq file = ['SRX2250627.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:09:20: # Command line: callpeak -t SRX2250627.bam -f BAM -g 12100000 -n SRX2250627.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2250627.20 # format = BAM # ChIP-seq file = ['SRX2250627.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:09:20: # Command line: callpeak -t SRX2250627.bam -f BAM -g 12100000 -n SRX2250627.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2250627.05 # format = BAM # ChIP-seq file = ['SRX2250627.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:09:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:09:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:09:20: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:09:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:09:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:09:20: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:09:29: 1000000 INFO @ Wed, 28 Jun 2017 08:09:29: 1000000 INFO @ Wed, 28 Jun 2017 08:09:30: 1000000 INFO @ Wed, 28 Jun 2017 08:09:39: 2000000 INFO @ Wed, 28 Jun 2017 08:09:39: 2000000 INFO @ Wed, 28 Jun 2017 08:09:41: 2000000 INFO @ Wed, 28 Jun 2017 08:09:48: 3000000 INFO @ Wed, 28 Jun 2017 08:09:48: 3000000 INFO @ Wed, 28 Jun 2017 08:09:51: 3000000 INFO @ Wed, 28 Jun 2017 08:09:58: 4000000 INFO @ Wed, 28 Jun 2017 08:09:58: 4000000 INFO @ Wed, 28 Jun 2017 08:10:01: 4000000 INFO @ Wed, 28 Jun 2017 08:10:08: 5000000 INFO @ Wed, 28 Jun 2017 08:10:08: 5000000 INFO @ Wed, 28 Jun 2017 08:10:11: 5000000 INFO @ Wed, 28 Jun 2017 08:10:18: 6000000 INFO @ Wed, 28 Jun 2017 08:10:18: 6000000 INFO @ Wed, 28 Jun 2017 08:10:21: 6000000 INFO @ Wed, 28 Jun 2017 08:10:28: 7000000 INFO @ Wed, 28 Jun 2017 08:10:28: 7000000 INFO @ Wed, 28 Jun 2017 08:10:31: 7000000 INFO @ Wed, 28 Jun 2017 08:10:38: 8000000 INFO @ Wed, 28 Jun 2017 08:10:38: 8000000 INFO @ Wed, 28 Jun 2017 08:10:41: 8000000 INFO @ Wed, 28 Jun 2017 08:10:49: 9000000 INFO @ Wed, 28 Jun 2017 08:10:49: 9000000 INFO @ Wed, 28 Jun 2017 08:10:50: 9000000 INFO @ Wed, 28 Jun 2017 08:10:59: 10000000 INFO @ Wed, 28 Jun 2017 08:10:59: 10000000 INFO @ Wed, 28 Jun 2017 08:11:00: 10000000 INFO @ Wed, 28 Jun 2017 08:11:09: 11000000 INFO @ Wed, 28 Jun 2017 08:11:10: 11000000 INFO @ Wed, 28 Jun 2017 08:11:10: 11000000 INFO @ Wed, 28 Jun 2017 08:11:18: 12000000 INFO @ Wed, 28 Jun 2017 08:11:20: 12000000 INFO @ Wed, 28 Jun 2017 08:11:20: 12000000 INFO @ Wed, 28 Jun 2017 08:11:28: 13000000 INFO @ Wed, 28 Jun 2017 08:11:31: 13000000 INFO @ Wed, 28 Jun 2017 08:11:31: 13000000 INFO @ Wed, 28 Jun 2017 08:11:38: 14000000 INFO @ Wed, 28 Jun 2017 08:11:41: 14000000 INFO @ Wed, 28 Jun 2017 08:11:41: 14000000 INFO @ Wed, 28 Jun 2017 08:11:47: 15000000 INFO @ Wed, 28 Jun 2017 08:11:51: 15000000 INFO @ Wed, 28 Jun 2017 08:11:52: 15000000 INFO @ Wed, 28 Jun 2017 08:11:57: 16000000 INFO @ Wed, 28 Jun 2017 08:12:01: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 28 Jun 2017 08:12:02: 16000000 INFO @ Wed, 28 Jun 2017 08:12:07: 17000000 INFO @ Wed, 28 Jun 2017 08:12:11: 17000000 INFO @ Wed, 28 Jun 2017 08:12:12: 17000000 BigWig に変換しました。 INFO @ Wed, 28 Jun 2017 08:12:14: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:12:14: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:12:14: #1 total tags in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:14: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:12:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:12:15: #1 tags after filtering in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:12:15: #1 finished! INFO @ Wed, 28 Jun 2017 08:12:15: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:12:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:12:16: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:12:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:12:16: Process for pairing-model is terminated! cat: SRX2250627.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250627.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 08:12:18: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:12:18: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:12:18: #1 total tags in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:18: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:12:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:12:18: #1 tags after filtering in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:18: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:12:18: #1 finished! INFO @ Wed, 28 Jun 2017 08:12:18: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:12:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:12:19: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:12:19: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:12:19: #1 total tags in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:12:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:12:19: #1 tags after filtering in treatment: 17714454 INFO @ Wed, 28 Jun 2017 08:12:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:12:19: #1 finished! INFO @ Wed, 28 Jun 2017 08:12:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:12:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:12:19: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:12:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:12:19: Process for pairing-model is terminated! cat: SRX2250627.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250627.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 08:12:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:12:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:12:20: Process for pairing-model is terminated! cat: SRX2250627.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250627.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250627.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling