Job ID = 9162397 sra ファイルのダウンロード中... Completed: 4270505K bytes transferred in 36 seconds (968920K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 58815238 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2250626/SRR4429107.sra Written 58815238 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:59 58815238 reads; of these: 58815238 (100.00%) were unpaired; of these: 2994581 (5.09%) aligned 0 times 43508766 (73.98%) aligned exactly 1 time 12311891 (20.93%) aligned >1 times 94.91% overall alignment rate Time searching: 00:18:59 Overall time: 00:18:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 36248884 / 55820657 = 0.6494 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 08:19:27: # Command line: callpeak -t SRX2250626.bam -f BAM -g 12100000 -n SRX2250626.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2250626.20 # format = BAM # ChIP-seq file = ['SRX2250626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:19:27: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:19:27: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:19:27: # Command line: callpeak -t SRX2250626.bam -f BAM -g 12100000 -n SRX2250626.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2250626.05 # format = BAM # ChIP-seq file = ['SRX2250626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:19:27: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:19:27: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:19:27: # Command line: callpeak -t SRX2250626.bam -f BAM -g 12100000 -n SRX2250626.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2250626.10 # format = BAM # ChIP-seq file = ['SRX2250626.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 08:19:27: #1 read tag files... INFO @ Wed, 28 Jun 2017 08:19:27: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 08:19:37: 1000000 INFO @ Wed, 28 Jun 2017 08:19:38: 1000000 INFO @ Wed, 28 Jun 2017 08:19:38: 1000000 INFO @ Wed, 28 Jun 2017 08:19:47: 2000000 INFO @ Wed, 28 Jun 2017 08:19:48: 2000000 INFO @ Wed, 28 Jun 2017 08:19:48: 2000000 INFO @ Wed, 28 Jun 2017 08:19:58: 3000000 INFO @ Wed, 28 Jun 2017 08:20:00: 3000000 INFO @ Wed, 28 Jun 2017 08:20:00: 3000000 INFO @ Wed, 28 Jun 2017 08:20:10: 4000000 INFO @ Wed, 28 Jun 2017 08:20:12: 4000000 INFO @ Wed, 28 Jun 2017 08:20:12: 4000000 INFO @ Wed, 28 Jun 2017 08:20:22: 5000000 INFO @ Wed, 28 Jun 2017 08:20:24: 5000000 INFO @ Wed, 28 Jun 2017 08:20:24: 5000000 INFO @ Wed, 28 Jun 2017 08:20:32: 6000000 INFO @ Wed, 28 Jun 2017 08:20:35: 6000000 INFO @ Wed, 28 Jun 2017 08:20:35: 6000000 INFO @ Wed, 28 Jun 2017 08:20:43: 7000000 INFO @ Wed, 28 Jun 2017 08:20:46: 7000000 INFO @ Wed, 28 Jun 2017 08:20:46: 7000000 INFO @ Wed, 28 Jun 2017 08:20:53: 8000000 INFO @ Wed, 28 Jun 2017 08:20:57: 8000000 INFO @ Wed, 28 Jun 2017 08:20:57: 8000000 INFO @ Wed, 28 Jun 2017 08:21:04: 9000000 INFO @ Wed, 28 Jun 2017 08:21:08: 9000000 INFO @ Wed, 28 Jun 2017 08:21:08: 9000000 INFO @ Wed, 28 Jun 2017 08:21:14: 10000000 INFO @ Wed, 28 Jun 2017 08:21:19: 10000000 INFO @ Wed, 28 Jun 2017 08:21:19: 10000000 INFO @ Wed, 28 Jun 2017 08:21:25: 11000000 INFO @ Wed, 28 Jun 2017 08:21:30: 11000000 INFO @ Wed, 28 Jun 2017 08:21:30: 11000000 INFO @ Wed, 28 Jun 2017 08:21:36: 12000000 INFO @ Wed, 28 Jun 2017 08:21:41: 12000000 INFO @ Wed, 28 Jun 2017 08:21:41: 12000000 INFO @ Wed, 28 Jun 2017 08:21:47: 13000000 INFO @ Wed, 28 Jun 2017 08:21:52: 13000000 INFO @ Wed, 28 Jun 2017 08:21:52: 13000000 INFO @ Wed, 28 Jun 2017 08:21:58: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 28 Jun 2017 08:22:04: 14000000 INFO @ Wed, 28 Jun 2017 08:22:04: 14000000 INFO @ Wed, 28 Jun 2017 08:22:09: 15000000 BigWig に変換しました。 INFO @ Wed, 28 Jun 2017 08:22:15: 15000000 INFO @ Wed, 28 Jun 2017 08:22:15: 15000000 INFO @ Wed, 28 Jun 2017 08:22:20: 16000000 INFO @ Wed, 28 Jun 2017 08:22:26: 16000000 INFO @ Wed, 28 Jun 2017 08:22:27: 16000000 INFO @ Wed, 28 Jun 2017 08:22:32: 17000000 INFO @ Wed, 28 Jun 2017 08:22:37: 17000000 INFO @ Wed, 28 Jun 2017 08:22:38: 17000000 INFO @ Wed, 28 Jun 2017 08:22:44: 18000000 INFO @ Wed, 28 Jun 2017 08:22:48: 18000000 INFO @ Wed, 28 Jun 2017 08:22:49: 18000000 INFO @ Wed, 28 Jun 2017 08:22:55: 19000000 INFO @ Wed, 28 Jun 2017 08:22:59: 19000000 INFO @ Wed, 28 Jun 2017 08:22:59: 19000000 INFO @ Wed, 28 Jun 2017 08:23:02: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:23:02: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:23:02: #1 total tags in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:02: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:23:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:23:02: #1 tags after filtering in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:23:02: #1 finished! INFO @ Wed, 28 Jun 2017 08:23:02: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:23:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:23:04: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:23:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:23:04: Process for pairing-model is terminated! cat: SRX2250626.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250626.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 08:23:05: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:23:05: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:23:05: #1 total tags in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:23:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:23:05: #1 tag size is determined as 100 bps INFO @ Wed, 28 Jun 2017 08:23:05: #1 tag size = 100 INFO @ Wed, 28 Jun 2017 08:23:05: #1 total tags in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:05: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 08:23:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 08:23:05: #1 tags after filtering in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:23:05: #1 finished! INFO @ Wed, 28 Jun 2017 08:23:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:23:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:23:05: #1 tags after filtering in treatment: 19571773 INFO @ Wed, 28 Jun 2017 08:23:05: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 08:23:05: #1 finished! INFO @ Wed, 28 Jun 2017 08:23:05: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 08:23:05: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 08:23:06: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:23:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:23:06: Process for pairing-model is terminated! cat: SRX2250626.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250626.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 08:23:07: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 08:23:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 08:23:07: Process for pairing-model is terminated! cat: SRX2250626.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2250626.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2250626.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling