Job ID = 2009905 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T11:43:27 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 23,767,356 reads read : 23,767,356 reads written : 23,767,356 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:04:06 23767356 reads; of these: 23767356 (100.00%) were unpaired; of these: 1179348 (4.96%) aligned 0 times 19412094 (81.68%) aligned exactly 1 time 3175914 (13.36%) aligned >1 times 95.04% overall alignment rate Time searching: 00:04:07 Overall time: 00:04:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10003103 / 22588008 = 0.4429 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:55:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:55:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:55:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:55:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:55:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:55:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:55:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:55:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:55:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:55:44: 1000000 INFO @ Fri, 05 Jul 2019 20:55:46: 1000000 INFO @ Fri, 05 Jul 2019 20:55:47: 1000000 INFO @ Fri, 05 Jul 2019 20:55:52: 2000000 INFO @ Fri, 05 Jul 2019 20:55:53: 2000000 INFO @ Fri, 05 Jul 2019 20:55:55: 2000000 INFO @ Fri, 05 Jul 2019 20:56:00: 3000000 INFO @ Fri, 05 Jul 2019 20:56:00: 3000000 INFO @ Fri, 05 Jul 2019 20:56:03: 3000000 INFO @ Fri, 05 Jul 2019 20:56:08: 4000000 INFO @ Fri, 05 Jul 2019 20:56:09: 4000000 INFO @ Fri, 05 Jul 2019 20:56:10: 4000000 INFO @ Fri, 05 Jul 2019 20:56:15: 5000000 INFO @ Fri, 05 Jul 2019 20:56:17: 5000000 INFO @ Fri, 05 Jul 2019 20:56:18: 5000000 INFO @ Fri, 05 Jul 2019 20:56:22: 6000000 INFO @ Fri, 05 Jul 2019 20:56:25: 6000000 INFO @ Fri, 05 Jul 2019 20:56:26: 6000000 INFO @ Fri, 05 Jul 2019 20:56:29: 7000000 INFO @ Fri, 05 Jul 2019 20:56:33: 7000000 INFO @ Fri, 05 Jul 2019 20:56:34: 7000000 INFO @ Fri, 05 Jul 2019 20:56:36: 8000000 INFO @ Fri, 05 Jul 2019 20:56:42: 8000000 INFO @ Fri, 05 Jul 2019 20:56:42: 8000000 INFO @ Fri, 05 Jul 2019 20:56:43: 9000000 INFO @ Fri, 05 Jul 2019 20:56:50: 9000000 INFO @ Fri, 05 Jul 2019 20:56:50: 9000000 INFO @ Fri, 05 Jul 2019 20:56:51: 10000000 INFO @ Fri, 05 Jul 2019 20:56:58: 11000000 INFO @ Fri, 05 Jul 2019 20:56:59: 10000000 INFO @ Fri, 05 Jul 2019 20:56:59: 10000000 INFO @ Fri, 05 Jul 2019 20:57:06: 12000000 INFO @ Fri, 05 Jul 2019 20:57:07: 11000000 INFO @ Fri, 05 Jul 2019 20:57:08: 11000000 INFO @ Fri, 05 Jul 2019 20:57:10: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:57:10: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:57:10: #1 total tags in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:10: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:57:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:57:10: #1 tags after filtering in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:57:10: #1 finished! INFO @ Fri, 05 Jul 2019 20:57:10: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:57:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:57:11: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:57:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:57:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:57:16: 12000000 INFO @ Fri, 05 Jul 2019 20:57:16: 12000000 INFO @ Fri, 05 Jul 2019 20:57:20: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:57:20: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:57:20: #1 total tags in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:57:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:57:20: #1 tags after filtering in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:57:20: #1 finished! INFO @ Fri, 05 Jul 2019 20:57:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:57:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:57:21: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:57:21: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:57:21: #1 total tags in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:57:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:57:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:57:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:57:21: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 20:57:21: #1 tags after filtering in treatment: 12584905 INFO @ Fri, 05 Jul 2019 20:57:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:57:21: #1 finished! INFO @ Fri, 05 Jul 2019 20:57:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:57:21: #2 looking for paired plus/minus strand peaks... cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:57:22: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:57:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:57:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148754/SRX2148754.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。