Job ID = 2009902 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 23,624,441 reads read : 23,624,441 reads written : 23,624,441 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:03 23624441 reads; of these: 23624441 (100.00%) were unpaired; of these: 828949 (3.51%) aligned 0 times 18298404 (77.46%) aligned exactly 1 time 4497088 (19.04%) aligned >1 times 96.49% overall alignment rate Time searching: 00:04:03 Overall time: 00:04:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10639847 / 22795492 = 0.4668 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:53:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:53:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:53:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:53:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:53:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:53:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:53:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:53:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:53:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:53:25: 1000000 INFO @ Fri, 05 Jul 2019 20:53:27: 1000000 INFO @ Fri, 05 Jul 2019 20:53:28: 1000000 INFO @ Fri, 05 Jul 2019 20:53:33: 2000000 INFO @ Fri, 05 Jul 2019 20:53:37: 2000000 INFO @ Fri, 05 Jul 2019 20:53:38: 2000000 INFO @ Fri, 05 Jul 2019 20:53:39: 3000000 INFO @ Fri, 05 Jul 2019 20:53:46: 4000000 INFO @ Fri, 05 Jul 2019 20:53:47: 3000000 INFO @ Fri, 05 Jul 2019 20:53:48: 3000000 INFO @ Fri, 05 Jul 2019 20:53:53: 5000000 INFO @ Fri, 05 Jul 2019 20:53:57: 4000000 INFO @ Fri, 05 Jul 2019 20:53:58: 4000000 INFO @ Fri, 05 Jul 2019 20:54:00: 6000000 INFO @ Fri, 05 Jul 2019 20:54:07: 5000000 INFO @ Fri, 05 Jul 2019 20:54:07: 7000000 INFO @ Fri, 05 Jul 2019 20:54:08: 5000000 INFO @ Fri, 05 Jul 2019 20:54:14: 8000000 INFO @ Fri, 05 Jul 2019 20:54:16: 6000000 INFO @ Fri, 05 Jul 2019 20:54:17: 6000000 INFO @ Fri, 05 Jul 2019 20:54:21: 9000000 INFO @ Fri, 05 Jul 2019 20:54:26: 7000000 INFO @ Fri, 05 Jul 2019 20:54:27: 7000000 INFO @ Fri, 05 Jul 2019 20:54:28: 10000000 INFO @ Fri, 05 Jul 2019 20:54:35: 11000000 INFO @ Fri, 05 Jul 2019 20:54:36: 8000000 INFO @ Fri, 05 Jul 2019 20:54:37: 8000000 INFO @ Fri, 05 Jul 2019 20:54:42: 12000000 INFO @ Fri, 05 Jul 2019 20:54:43: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:54:43: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:54:43: #1 total tags in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:54:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:54:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:54:43: #1 tags after filtering in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:54:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:54:43: #1 finished! INFO @ Fri, 05 Jul 2019 20:54:43: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:54:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:54:44: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:54:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:54:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:54:45: 9000000 INFO @ Fri, 05 Jul 2019 20:54:46: 9000000 INFO @ Fri, 05 Jul 2019 20:54:55: 10000000 INFO @ Fri, 05 Jul 2019 20:54:56: 10000000 INFO @ Fri, 05 Jul 2019 20:55:05: 11000000 INFO @ Fri, 05 Jul 2019 20:55:06: 11000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 20:55:14: 12000000 INFO @ Fri, 05 Jul 2019 20:55:15: 12000000 INFO @ Fri, 05 Jul 2019 20:55:16: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:55:16: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:55:16: #1 total tags in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:55:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:55:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:55:16: #1 tags after filtering in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:55:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:55:16: #1 finished! INFO @ Fri, 05 Jul 2019 20:55:16: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:55:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:55:16: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:55:16: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:55:16: #1 total tags in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:55:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:55:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:55:17: #1 tags after filtering in treatment: 12155645 INFO @ Fri, 05 Jul 2019 20:55:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:55:17: #1 finished! INFO @ Fri, 05 Jul 2019 20:55:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:55:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:55:17: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:55:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:55:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:55:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:55:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:55:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148752/SRX2148752.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。