Job ID = 2009900 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,809,208 reads read : 22,809,208 reads written : 22,809,208 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:09 22809208 reads; of these: 22809208 (100.00%) were unpaired; of these: 852824 (3.74%) aligned 0 times 17920632 (78.57%) aligned exactly 1 time 4035752 (17.69%) aligned >1 times 96.26% overall alignment rate Time searching: 00:04:09 Overall time: 00:04:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10106419 / 21956384 = 0.4603 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:50:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:50:47: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:50:47: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:50:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:50:48: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:50:48: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:50:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:50:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:50:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:50:57: 1000000 INFO @ Fri, 05 Jul 2019 20:50:57: 1000000 INFO @ Fri, 05 Jul 2019 20:50:58: 1000000 INFO @ Fri, 05 Jul 2019 20:51:07: 2000000 INFO @ Fri, 05 Jul 2019 20:51:07: 2000000 INFO @ Fri, 05 Jul 2019 20:51:07: 2000000 INFO @ Fri, 05 Jul 2019 20:51:16: 3000000 INFO @ Fri, 05 Jul 2019 20:51:17: 3000000 INFO @ Fri, 05 Jul 2019 20:51:17: 3000000 INFO @ Fri, 05 Jul 2019 20:51:25: 4000000 INFO @ Fri, 05 Jul 2019 20:51:25: 4000000 INFO @ Fri, 05 Jul 2019 20:51:26: 4000000 INFO @ Fri, 05 Jul 2019 20:51:33: 5000000 INFO @ Fri, 05 Jul 2019 20:51:34: 5000000 INFO @ Fri, 05 Jul 2019 20:51:36: 5000000 INFO @ Fri, 05 Jul 2019 20:51:42: 6000000 INFO @ Fri, 05 Jul 2019 20:51:43: 6000000 INFO @ Fri, 05 Jul 2019 20:51:45: 6000000 INFO @ Fri, 05 Jul 2019 20:51:50: 7000000 INFO @ Fri, 05 Jul 2019 20:51:52: 7000000 INFO @ Fri, 05 Jul 2019 20:51:55: 7000000 INFO @ Fri, 05 Jul 2019 20:51:59: 8000000 INFO @ Fri, 05 Jul 2019 20:52:00: 8000000 INFO @ Fri, 05 Jul 2019 20:52:05: 8000000 INFO @ Fri, 05 Jul 2019 20:52:08: 9000000 INFO @ Fri, 05 Jul 2019 20:52:09: 9000000 INFO @ Fri, 05 Jul 2019 20:52:14: 9000000 INFO @ Fri, 05 Jul 2019 20:52:16: 10000000 INFO @ Fri, 05 Jul 2019 20:52:18: 10000000 INFO @ Fri, 05 Jul 2019 20:52:24: 10000000 INFO @ Fri, 05 Jul 2019 20:52:25: 11000000 INFO @ Fri, 05 Jul 2019 20:52:26: 11000000 INFO @ Fri, 05 Jul 2019 20:52:32: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:52:32: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:52:32: #1 total tags in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:32: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:52:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:52:32: #1 tags after filtering in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:52:32: #1 finished! INFO @ Fri, 05 Jul 2019 20:52:32: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:52:32: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:52:33: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:52:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:52:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:52:33: 11000000 INFO @ Fri, 05 Jul 2019 20:52:33: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:52:33: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:52:33: #1 total tags in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:52:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:52:34: #1 tags after filtering in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:52:34: #1 finished! INFO @ Fri, 05 Jul 2019 20:52:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:52:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:52:34: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:52:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:52:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:52:41: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:52:41: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:52:41: #1 total tags in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:41: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:52:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:52:41: #1 tags after filtering in treatment: 11849965 INFO @ Fri, 05 Jul 2019 20:52:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:52:41: #1 finished! INFO @ Fri, 05 Jul 2019 20:52:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:52:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:52:42: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:52:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:52:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148750/SRX2148750.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。