Job ID = 2009894 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,843,006 reads read : 14,843,006 reads written : 14,843,006 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:38 14843006 reads; of these: 14843006 (100.00%) were unpaired; of these: 349734 (2.36%) aligned 0 times 12339774 (83.14%) aligned exactly 1 time 2153498 (14.51%) aligned >1 times 97.64% overall alignment rate Time searching: 00:02:38 Overall time: 00:02:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5462962 / 14493272 = 0.3769 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:47:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:47:47: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:47:47: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:47:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:47:48: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:47:48: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:47:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:47:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:47:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:47:57: 1000000 INFO @ Fri, 05 Jul 2019 20:47:57: 1000000 INFO @ Fri, 05 Jul 2019 20:47:58: 1000000 INFO @ Fri, 05 Jul 2019 20:48:05: 2000000 INFO @ Fri, 05 Jul 2019 20:48:07: 2000000 INFO @ Fri, 05 Jul 2019 20:48:08: 2000000 INFO @ Fri, 05 Jul 2019 20:48:13: 3000000 INFO @ Fri, 05 Jul 2019 20:48:16: 3000000 INFO @ Fri, 05 Jul 2019 20:48:18: 3000000 INFO @ Fri, 05 Jul 2019 20:48:21: 4000000 INFO @ Fri, 05 Jul 2019 20:48:24: 4000000 INFO @ Fri, 05 Jul 2019 20:48:28: 4000000 INFO @ Fri, 05 Jul 2019 20:48:29: 5000000 INFO @ Fri, 05 Jul 2019 20:48:33: 5000000 INFO @ Fri, 05 Jul 2019 20:48:38: 5000000 INFO @ Fri, 05 Jul 2019 20:48:38: 6000000 INFO @ Fri, 05 Jul 2019 20:48:42: 6000000 INFO @ Fri, 05 Jul 2019 20:48:45: 7000000 INFO @ Fri, 05 Jul 2019 20:48:47: 6000000 INFO @ Fri, 05 Jul 2019 20:48:50: 7000000 INFO @ Fri, 05 Jul 2019 20:48:53: 8000000 INFO @ Fri, 05 Jul 2019 20:48:57: 7000000 INFO @ Fri, 05 Jul 2019 20:48:59: 8000000 INFO @ Fri, 05 Jul 2019 20:49:00: 9000000 INFO @ Fri, 05 Jul 2019 20:49:00: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:49:00: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:49:00: #1 total tags in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:49:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:49:01: #1 tags after filtering in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:49:01: #1 finished! INFO @ Fri, 05 Jul 2019 20:49:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:49:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:49:01: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:49:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:49:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:49:07: 9000000 INFO @ Fri, 05 Jul 2019 20:49:07: 8000000 INFO @ Fri, 05 Jul 2019 20:49:07: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:49:07: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:49:07: #1 total tags in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:07: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:49:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:49:08: #1 tags after filtering in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:49:08: #1 finished! INFO @ Fri, 05 Jul 2019 20:49:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:49:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:49:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:49:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:49:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:49:17: 9000000 INFO @ Fri, 05 Jul 2019 20:49:17: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:49:17: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:49:17: #1 total tags in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:17: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:49:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:49:17: #1 tags after filtering in treatment: 9030310 INFO @ Fri, 05 Jul 2019 20:49:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:49:17: #1 finished! INFO @ Fri, 05 Jul 2019 20:49:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:49:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:49:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:49:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:49:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148744/SRX2148744.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。