Job ID = 2009883 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,323,761 reads read : 12,323,761 reads written : 12,323,761 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:10 12323761 reads; of these: 12323761 (100.00%) were unpaired; of these: 362017 (2.94%) aligned 0 times 9878920 (80.16%) aligned exactly 1 time 2082824 (16.90%) aligned >1 times 97.06% overall alignment rate Time searching: 00:02:10 Overall time: 00:02:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3806487 / 11961744 = 0.3182 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:32:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:32:54: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:32:54: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:32:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:32:55: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:32:55: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:32:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:32:56: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:32:56: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:33:02: 1000000 INFO @ Fri, 05 Jul 2019 20:33:03: 1000000 INFO @ Fri, 05 Jul 2019 20:33:03: 1000000 INFO @ Fri, 05 Jul 2019 20:33:09: 2000000 INFO @ Fri, 05 Jul 2019 20:33:11: 2000000 INFO @ Fri, 05 Jul 2019 20:33:11: 2000000 INFO @ Fri, 05 Jul 2019 20:33:16: 3000000 INFO @ Fri, 05 Jul 2019 20:33:17: 3000000 INFO @ Fri, 05 Jul 2019 20:33:19: 3000000 INFO @ Fri, 05 Jul 2019 20:33:22: 4000000 INFO @ Fri, 05 Jul 2019 20:33:24: 4000000 INFO @ Fri, 05 Jul 2019 20:33:26: 4000000 INFO @ Fri, 05 Jul 2019 20:33:29: 5000000 INFO @ Fri, 05 Jul 2019 20:33:31: 5000000 INFO @ Fri, 05 Jul 2019 20:33:34: 5000000 INFO @ Fri, 05 Jul 2019 20:33:36: 6000000 INFO @ Fri, 05 Jul 2019 20:33:38: 6000000 INFO @ Fri, 05 Jul 2019 20:33:41: 6000000 INFO @ Fri, 05 Jul 2019 20:33:43: 7000000 INFO @ Fri, 05 Jul 2019 20:33:45: 7000000 INFO @ Fri, 05 Jul 2019 20:33:49: 7000000 INFO @ Fri, 05 Jul 2019 20:33:50: 8000000 INFO @ Fri, 05 Jul 2019 20:33:51: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:33:51: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:33:51: #1 total tags in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:51: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:33:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:33:51: #1 tags after filtering in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:33:51: #1 finished! INFO @ Fri, 05 Jul 2019 20:33:51: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:33:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:33:51: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:33:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:33:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:33:52: 8000000 INFO @ Fri, 05 Jul 2019 20:33:53: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:33:53: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:33:53: #1 total tags in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:33:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:33:53: #1 tags after filtering in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:33:53: #1 finished! INFO @ Fri, 05 Jul 2019 20:33:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:33:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:33:54: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:33:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:33:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:33:56: 8000000 INFO @ Fri, 05 Jul 2019 20:33:57: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 20:33:57: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 20:33:57: #1 total tags in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:57: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:33:57: #1 tags after filtering in treatment: 8155257 INFO @ Fri, 05 Jul 2019 20:33:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:33:57: #1 finished! INFO @ Fri, 05 Jul 2019 20:33:57: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:33:58: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:33:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:33:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 49 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2148734/SRX2148734.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。