Job ID = 9162344 sra ファイルのダウンロード中... Completed: 935584K bytes transferred in 10 seconds (734825K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28295702 spots for /home/okishinya/chipatlas/results/sacCer3/SRX211376/SRR636627.sra Written 28295702 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:42 28295702 reads; of these: 28295702 (100.00%) were unpaired; of these: 9464852 (33.45%) aligned 0 times 16283433 (57.55%) aligned exactly 1 time 2547417 (9.00%) aligned >1 times 66.55% overall alignment rate Time searching: 00:06:42 Overall time: 00:06:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11225155 / 18830850 = 0.5961 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:39:36: # Command line: callpeak -t SRX211376.bam -f BAM -g 12100000 -n SRX211376.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX211376.20 # format = BAM # ChIP-seq file = ['SRX211376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:39:36: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:39:36: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:39:36: # Command line: callpeak -t SRX211376.bam -f BAM -g 12100000 -n SRX211376.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX211376.10 # format = BAM # ChIP-seq file = ['SRX211376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:39:36: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:39:36: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:39:36: # Command line: callpeak -t SRX211376.bam -f BAM -g 12100000 -n SRX211376.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX211376.05 # format = BAM # ChIP-seq file = ['SRX211376.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:39:36: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:39:36: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:39:44: 1000000 INFO @ Wed, 28 Jun 2017 07:39:44: 1000000 INFO @ Wed, 28 Jun 2017 07:39:44: 1000000 INFO @ Wed, 28 Jun 2017 07:39:52: 2000000 INFO @ Wed, 28 Jun 2017 07:39:52: 2000000 INFO @ Wed, 28 Jun 2017 07:39:53: 2000000 INFO @ Wed, 28 Jun 2017 07:40:00: 3000000 INFO @ Wed, 28 Jun 2017 07:40:00: 3000000 INFO @ Wed, 28 Jun 2017 07:40:02: 3000000 INFO @ Wed, 28 Jun 2017 07:40:07: 4000000 INFO @ Wed, 28 Jun 2017 07:40:07: 4000000 INFO @ Wed, 28 Jun 2017 07:40:10: 4000000 INFO @ Wed, 28 Jun 2017 07:40:15: 5000000 INFO @ Wed, 28 Jun 2017 07:40:15: 5000000 INFO @ Wed, 28 Jun 2017 07:40:19: 5000000 INFO @ Wed, 28 Jun 2017 07:40:23: 6000000 INFO @ Wed, 28 Jun 2017 07:40:23: 6000000 INFO @ Wed, 28 Jun 2017 07:40:27: 6000000 INFO @ Wed, 28 Jun 2017 07:40:31: 7000000 INFO @ Wed, 28 Jun 2017 07:40:31: 7000000 INFO @ Wed, 28 Jun 2017 07:40:36: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:40:36: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:40:36: #1 total tags in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:36: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:40:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:40:36: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:40:36: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:40:36: #1 total tags in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:36: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:40:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:40:36: #1 tags after filtering in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:40:36: #1 finished! INFO @ Wed, 28 Jun 2017 07:40:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:40:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:40:36: #1 tags after filtering in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:36: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:40:36: #1 finished! INFO @ Wed, 28 Jun 2017 07:40:36: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:40:36: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:40:36: 7000000 INFO @ Wed, 28 Jun 2017 07:40:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:40:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:40:36: Process for pairing-model is terminated! cat: SRX211376.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Wed, 28 Jun 2017 07:40:36: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:40:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:40:36: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX211376.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX211376.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX211376.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:40:41: #1 tag size is determined as 51 bps INFO @ Wed, 28 Jun 2017 07:40:41: #1 tag size = 51 INFO @ Wed, 28 Jun 2017 07:40:41: #1 total tags in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:41: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:40:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:40:41: #1 tags after filtering in treatment: 7605695 INFO @ Wed, 28 Jun 2017 07:40:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:40:41: #1 finished! INFO @ Wed, 28 Jun 2017 07:40:41: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:40:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:40:42: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:40:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:40:42: Process for pairing-model is terminated! cat: SRX211376.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX211376.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX211376.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。