Job ID = 2009871 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,432,702 reads read : 36,432,702 reads written : 36,432,702 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR4105768.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:41 36432702 reads; of these: 36432702 (100.00%) were unpaired; of these: 1725989 (4.74%) aligned 0 times 22537637 (61.86%) aligned exactly 1 time 12169076 (33.40%) aligned >1 times 95.26% overall alignment rate Time searching: 00:07:42 Overall time: 00:07:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 21354347 / 34706713 = 0.6153 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:33:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:33:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:33:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:33:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:33:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:33:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:33:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:33:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:33:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:33:40: 1000000 INFO @ Fri, 05 Jul 2019 20:33:41: 1000000 INFO @ Fri, 05 Jul 2019 20:33:41: 1000000 INFO @ Fri, 05 Jul 2019 20:33:47: 2000000 INFO @ Fri, 05 Jul 2019 20:33:48: 2000000 INFO @ Fri, 05 Jul 2019 20:33:50: 2000000 INFO @ Fri, 05 Jul 2019 20:33:55: 3000000 INFO @ Fri, 05 Jul 2019 20:33:55: 3000000 INFO @ Fri, 05 Jul 2019 20:33:58: 3000000 INFO @ Fri, 05 Jul 2019 20:34:02: 4000000 INFO @ Fri, 05 Jul 2019 20:34:02: 4000000 INFO @ Fri, 05 Jul 2019 20:34:07: 4000000 INFO @ Fri, 05 Jul 2019 20:34:09: 5000000 INFO @ Fri, 05 Jul 2019 20:34:09: 5000000 INFO @ Fri, 05 Jul 2019 20:34:15: 5000000 INFO @ Fri, 05 Jul 2019 20:34:16: 6000000 INFO @ Fri, 05 Jul 2019 20:34:17: 6000000 INFO @ Fri, 05 Jul 2019 20:34:23: 7000000 INFO @ Fri, 05 Jul 2019 20:34:23: 6000000 INFO @ Fri, 05 Jul 2019 20:34:24: 7000000 INFO @ Fri, 05 Jul 2019 20:34:30: 8000000 INFO @ Fri, 05 Jul 2019 20:34:31: 8000000 INFO @ Fri, 05 Jul 2019 20:34:32: 7000000 INFO @ Fri, 05 Jul 2019 20:34:38: 9000000 INFO @ Fri, 05 Jul 2019 20:34:39: 9000000 INFO @ Fri, 05 Jul 2019 20:34:41: 8000000 INFO @ Fri, 05 Jul 2019 20:34:44: 10000000 INFO @ Fri, 05 Jul 2019 20:34:46: 10000000 INFO @ Fri, 05 Jul 2019 20:34:49: 9000000 INFO @ Fri, 05 Jul 2019 20:34:51: 11000000 INFO @ Fri, 05 Jul 2019 20:34:54: 11000000 INFO @ Fri, 05 Jul 2019 20:34:58: 10000000 INFO @ Fri, 05 Jul 2019 20:34:58: 12000000 INFO @ Fri, 05 Jul 2019 20:35:01: 12000000 INFO @ Fri, 05 Jul 2019 20:35:05: 13000000 INFO @ Fri, 05 Jul 2019 20:35:06: 11000000 INFO @ Fri, 05 Jul 2019 20:35:08: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:35:08: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:35:08: #1 total tags in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:35:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:35:08: #1 tags after filtering in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:35:08: #1 finished! INFO @ Fri, 05 Jul 2019 20:35:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:35:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:35:08: 13000000 INFO @ Fri, 05 Jul 2019 20:35:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:35:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:35:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:35:11: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:35:11: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:35:11: #1 total tags in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:35:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:35:11: #1 tags after filtering in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:35:11: #1 finished! INFO @ Fri, 05 Jul 2019 20:35:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:35:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:35:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:35:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:35:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:35:15: 12000000 INFO @ Fri, 05 Jul 2019 20:35:23: 13000000 INFO @ Fri, 05 Jul 2019 20:35:26: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 20:35:26: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 20:35:26: #1 total tags in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:26: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:35:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:35:26: #1 tags after filtering in treatment: 13352366 INFO @ Fri, 05 Jul 2019 20:35:26: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:35:26: #1 finished! INFO @ Fri, 05 Jul 2019 20:35:26: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:35:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:35:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:35:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:35:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070417/SRX2070417.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。