Job ID = 2009870 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,760,057 reads read : 41,760,057 reads written : 41,760,057 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:40 41760057 reads; of these: 41760057 (100.00%) were unpaired; of these: 1712899 (4.10%) aligned 0 times 24720346 (59.20%) aligned exactly 1 time 15326812 (36.70%) aligned >1 times 95.90% overall alignment rate Time searching: 00:07:40 Overall time: 00:07:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 25609773 / 40047158 = 0.6395 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:04:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:04:45: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:04:45: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:04:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:04:46: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:04:46: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:04:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:04:47: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:04:47: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:04:53: 1000000 INFO @ Fri, 05 Jul 2019 21:04:54: 1000000 INFO @ Fri, 05 Jul 2019 21:04:54: 1000000 INFO @ Fri, 05 Jul 2019 21:05:00: 2000000 INFO @ Fri, 05 Jul 2019 21:05:01: 2000000 INFO @ Fri, 05 Jul 2019 21:05:02: 2000000 INFO @ Fri, 05 Jul 2019 21:05:08: 3000000 INFO @ Fri, 05 Jul 2019 21:05:09: 3000000 INFO @ Fri, 05 Jul 2019 21:05:09: 3000000 INFO @ Fri, 05 Jul 2019 21:05:16: 4000000 INFO @ Fri, 05 Jul 2019 21:05:16: 4000000 INFO @ Fri, 05 Jul 2019 21:05:17: 4000000 INFO @ Fri, 05 Jul 2019 21:05:23: 5000000 INFO @ Fri, 05 Jul 2019 21:05:23: 5000000 INFO @ Fri, 05 Jul 2019 21:05:25: 5000000 INFO @ Fri, 05 Jul 2019 21:05:30: 6000000 INFO @ Fri, 05 Jul 2019 21:05:31: 6000000 INFO @ Fri, 05 Jul 2019 21:05:32: 6000000 INFO @ Fri, 05 Jul 2019 21:05:37: 7000000 INFO @ Fri, 05 Jul 2019 21:05:39: 7000000 INFO @ Fri, 05 Jul 2019 21:05:40: 7000000 INFO @ Fri, 05 Jul 2019 21:05:45: 8000000 INFO @ Fri, 05 Jul 2019 21:05:46: 8000000 INFO @ Fri, 05 Jul 2019 21:05:48: 8000000 INFO @ Fri, 05 Jul 2019 21:05:52: 9000000 INFO @ Fri, 05 Jul 2019 21:05:54: 9000000 INFO @ Fri, 05 Jul 2019 21:05:55: 9000000 INFO @ Fri, 05 Jul 2019 21:05:59: 10000000 INFO @ Fri, 05 Jul 2019 21:06:01: 10000000 INFO @ Fri, 05 Jul 2019 21:06:03: 10000000 INFO @ Fri, 05 Jul 2019 21:06:06: 11000000 INFO @ Fri, 05 Jul 2019 21:06:09: 11000000 INFO @ Fri, 05 Jul 2019 21:06:11: 11000000 INFO @ Fri, 05 Jul 2019 21:06:13: 12000000 INFO @ Fri, 05 Jul 2019 21:06:17: 12000000 INFO @ Fri, 05 Jul 2019 21:06:18: 12000000 INFO @ Fri, 05 Jul 2019 21:06:20: 13000000 INFO @ Fri, 05 Jul 2019 21:06:24: 13000000 INFO @ Fri, 05 Jul 2019 21:06:26: 13000000 INFO @ Fri, 05 Jul 2019 21:06:27: 14000000 INFO @ Fri, 05 Jul 2019 21:06:30: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:06:30: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:06:30: #1 total tags in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:30: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:06:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:06:31: #1 tags after filtering in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:06:31: #1 finished! INFO @ Fri, 05 Jul 2019 21:06:31: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:06:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:06:32: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:06:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:06:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 21:06:32: 14000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:06:34: 14000000 INFO @ Fri, 05 Jul 2019 21:06:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:06:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:06:35: #1 total tags in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:06:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:06:36: #1 tags after filtering in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:06:36: #1 finished! INFO @ Fri, 05 Jul 2019 21:06:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:06:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:06:37: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:06:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:06:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 21:06:37: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:06:37: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:06:37: #1 total tags in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:37: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:06:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:06:37: #1 tags after filtering in treatment: 14437385 INFO @ Fri, 05 Jul 2019 21:06:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:06:37: #1 finished! INFO @ Fri, 05 Jul 2019 21:06:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:06:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:06:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:06:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:06:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070416/SRX2070416.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。