Job ID = 2009869 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,611,597 reads read : 22,611,597 reads written : 22,611,597 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:54 22611597 reads; of these: 22611597 (100.00%) were unpaired; of these: 7526627 (33.29%) aligned 0 times 11638731 (51.47%) aligned exactly 1 time 3446239 (15.24%) aligned >1 times 66.71% overall alignment rate Time searching: 00:44:54 Overall time: 00:44:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7441151 / 15084970 = 0.4933 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:11:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:11:40: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:11:40: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:11:46: 1000000 INFO @ Fri, 05 Jul 2019 21:11:48: 1000000 INFO @ Fri, 05 Jul 2019 21:11:48: 1000000 INFO @ Fri, 05 Jul 2019 21:11:54: 2000000 INFO @ Fri, 05 Jul 2019 21:11:57: 2000000 INFO @ Fri, 05 Jul 2019 21:11:57: 2000000 INFO @ Fri, 05 Jul 2019 21:12:02: 3000000 INFO @ Fri, 05 Jul 2019 21:12:06: 3000000 INFO @ Fri, 05 Jul 2019 21:12:06: 3000000 INFO @ Fri, 05 Jul 2019 21:12:09: 4000000 INFO @ Fri, 05 Jul 2019 21:12:15: 4000000 INFO @ Fri, 05 Jul 2019 21:12:15: 4000000 INFO @ Fri, 05 Jul 2019 21:12:17: 5000000 INFO @ Fri, 05 Jul 2019 21:12:24: 5000000 INFO @ Fri, 05 Jul 2019 21:12:24: 5000000 INFO @ Fri, 05 Jul 2019 21:12:25: 6000000 INFO @ Fri, 05 Jul 2019 21:12:32: 6000000 INFO @ Fri, 05 Jul 2019 21:12:32: 6000000 INFO @ Fri, 05 Jul 2019 21:12:33: 7000000 INFO @ Fri, 05 Jul 2019 21:12:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:12:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:12:38: #1 total tags in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:12:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:12:38: #1 tags after filtering in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:12:38: #1 finished! INFO @ Fri, 05 Jul 2019 21:12:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:12:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:12:39: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:12:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:12:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:12:41: 7000000 INFO @ Fri, 05 Jul 2019 21:12:42: 7000000 INFO @ Fri, 05 Jul 2019 21:12:46: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:12:46: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:12:46: #1 total tags in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:12:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:12:47: #1 tags after filtering in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:12:47: #1 finished! INFO @ Fri, 05 Jul 2019 21:12:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:12:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:12:47: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:12:47: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:12:47: #1 total tags in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:12:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:12:47: #1 tags after filtering in treatment: 7643819 INFO @ Fri, 05 Jul 2019 21:12:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:12:47: #1 finished! INFO @ Fri, 05 Jul 2019 21:12:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:12:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:12:47: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:12:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:12:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:12:48: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:12:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:12:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2070415/SRX2070415.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。