Job ID = 7097579 SRX = SRX1967618 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 56091242 spots for SRR3938359/SRR3938359.sra Written 56091242 spots for SRR3938359/SRR3938359.sra Read 37701636 spots for SRR3938360/SRR3938360.sra Written 37701636 spots for SRR3938360/SRR3938360.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:56 93792878 reads; of these: 93792878 (100.00%) were unpaired; of these: 3387074 (3.61%) aligned 0 times 74755655 (79.70%) aligned exactly 1 time 15650149 (16.69%) aligned >1 times 96.39% overall alignment rate Time searching: 00:12:56 Overall time: 00:12:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 68189764 / 90405804 = 0.7543 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:08:26: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:08:26: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:08:32: 1000000 INFO @ Wed, 22 Jul 2020 12:08:38: 2000000 INFO @ Wed, 22 Jul 2020 12:08:43: 3000000 INFO @ Wed, 22 Jul 2020 12:08:49: 4000000 INFO @ Wed, 22 Jul 2020 12:08:54: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:08:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:08:56: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:08:56: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:09:00: 6000000 INFO @ Wed, 22 Jul 2020 12:09:02: 1000000 INFO @ Wed, 22 Jul 2020 12:09:05: 7000000 INFO @ Wed, 22 Jul 2020 12:09:08: 2000000 INFO @ Wed, 22 Jul 2020 12:09:11: 8000000 INFO @ Wed, 22 Jul 2020 12:09:13: 3000000 INFO @ Wed, 22 Jul 2020 12:09:17: 9000000 INFO @ Wed, 22 Jul 2020 12:09:19: 4000000 INFO @ Wed, 22 Jul 2020 12:09:23: 10000000 BedGraph に変換中... INFO @ Wed, 22 Jul 2020 12:09:25: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 12:09:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 12:09:26: #1 read tag files... INFO @ Wed, 22 Jul 2020 12:09:26: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 12:09:28: 11000000 INFO @ Wed, 22 Jul 2020 12:09:30: 6000000 INFO @ Wed, 22 Jul 2020 12:09:32: 1000000 INFO @ Wed, 22 Jul 2020 12:09:34: 12000000 INFO @ Wed, 22 Jul 2020 12:09:36: 7000000 INFO @ Wed, 22 Jul 2020 12:09:38: 2000000 INFO @ Wed, 22 Jul 2020 12:09:40: 13000000 INFO @ Wed, 22 Jul 2020 12:09:42: 8000000 INFO @ Wed, 22 Jul 2020 12:09:44: 3000000 INFO @ Wed, 22 Jul 2020 12:09:46: 14000000 INFO @ Wed, 22 Jul 2020 12:09:47: 9000000 INFO @ Wed, 22 Jul 2020 12:09:50: 4000000 INFO @ Wed, 22 Jul 2020 12:09:52: 15000000 INFO @ Wed, 22 Jul 2020 12:09:53: 10000000 INFO @ Wed, 22 Jul 2020 12:09:55: 5000000 INFO @ Wed, 22 Jul 2020 12:09:57: 16000000 INFO @ Wed, 22 Jul 2020 12:09:59: 11000000 INFO @ Wed, 22 Jul 2020 12:10:01: 6000000 INFO @ Wed, 22 Jul 2020 12:10:03: 17000000 INFO @ Wed, 22 Jul 2020 12:10:05: 12000000 INFO @ Wed, 22 Jul 2020 12:10:07: 7000000 INFO @ Wed, 22 Jul 2020 12:10:09: 18000000 INFO @ Wed, 22 Jul 2020 12:10:11: 13000000 INFO @ Wed, 22 Jul 2020 12:10:13: 8000000 INFO @ Wed, 22 Jul 2020 12:10:15: 19000000 INFO @ Wed, 22 Jul 2020 12:10:16: 14000000 INFO @ Wed, 22 Jul 2020 12:10:18: 9000000 INFO @ Wed, 22 Jul 2020 12:10:20: 20000000 INFO @ Wed, 22 Jul 2020 12:10:22: 15000000 INFO @ Wed, 22 Jul 2020 12:10:24: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 12:10:26: 21000000 INFO @ Wed, 22 Jul 2020 12:10:28: 16000000 INFO @ Wed, 22 Jul 2020 12:10:30: 11000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 12:10:32: 22000000 INFO @ Wed, 22 Jul 2020 12:10:33: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:10:33: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:10:33: #1 total tags in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:10:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:10:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:10:34: 17000000 INFO @ Wed, 22 Jul 2020 12:10:34: #1 tags after filtering in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:10:34: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:10:34: #1 finished! INFO @ Wed, 22 Jul 2020 12:10:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:10:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:10:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:10:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:10:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:10:36: 12000000 INFO @ Wed, 22 Jul 2020 12:10:40: 18000000 INFO @ Wed, 22 Jul 2020 12:10:42: 13000000 INFO @ Wed, 22 Jul 2020 12:10:45: 19000000 INFO @ Wed, 22 Jul 2020 12:10:48: 14000000 INFO @ Wed, 22 Jul 2020 12:10:51: 20000000 INFO @ Wed, 22 Jul 2020 12:10:54: 15000000 INFO @ Wed, 22 Jul 2020 12:10:57: 21000000 INFO @ Wed, 22 Jul 2020 12:11:00: 16000000 INFO @ Wed, 22 Jul 2020 12:11:02: 22000000 INFO @ Wed, 22 Jul 2020 12:11:04: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:11:04: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:11:04: #1 total tags in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:11:04: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:11:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:11:04: #1 tags after filtering in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:11:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:11:04: #1 finished! INFO @ Wed, 22 Jul 2020 12:11:04: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:11:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:11:05: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:11:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:11:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 12:11:05: 17000000 INFO @ Wed, 22 Jul 2020 12:11:11: 18000000 INFO @ Wed, 22 Jul 2020 12:11:16: 19000000 INFO @ Wed, 22 Jul 2020 12:11:22: 20000000 INFO @ Wed, 22 Jul 2020 12:11:27: 21000000 INFO @ Wed, 22 Jul 2020 12:11:32: 22000000 INFO @ Wed, 22 Jul 2020 12:11:33: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 12:11:33: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 12:11:33: #1 total tags in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:11:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 12:11:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 12:11:34: #1 tags after filtering in treatment: 22216040 INFO @ Wed, 22 Jul 2020 12:11:34: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 12:11:34: #1 finished! INFO @ Wed, 22 Jul 2020 12:11:34: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 12:11:34: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 12:11:35: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 12:11:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 12:11:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967618/SRX1967618.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling