Job ID = 7097552 SRX = SRX1967616 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 2121605 spots for SRR3938355/SRR3938355.sra Written 2121605 spots for SRR3938355/SRR3938355.sra Read 25899751 spots for SRR3938356/SRR3938356.sra Written 25899751 spots for SRR3938356/SRR3938356.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:27 28021356 reads; of these: 28021356 (100.00%) were unpaired; of these: 4817910 (17.19%) aligned 0 times 18437427 (65.80%) aligned exactly 1 time 4766019 (17.01%) aligned >1 times 82.81% overall alignment rate Time searching: 00:03:28 Overall time: 00:03:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15476182 / 23203446 = 0.6670 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:47:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:47:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:47:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:47:26: 1000000 INFO @ Wed, 22 Jul 2020 11:47:32: 2000000 INFO @ Wed, 22 Jul 2020 11:47:37: 3000000 INFO @ Wed, 22 Jul 2020 11:47:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:47:49: 5000000 INFO @ Wed, 22 Jul 2020 11:47:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:47:50: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:47:50: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:47:55: 1000000 INFO @ Wed, 22 Jul 2020 11:47:56: 6000000 INFO @ Wed, 22 Jul 2020 11:48:01: 2000000 INFO @ Wed, 22 Jul 2020 11:48:02: 7000000 INFO @ Wed, 22 Jul 2020 11:48:06: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 11:48:06: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 11:48:06: #1 total tags in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:48:06: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:48:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:48:07: #1 tags after filtering in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:48:07: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:48:07: #1 finished! INFO @ Wed, 22 Jul 2020 11:48:07: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:48:07: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:48:07: 3000000 INFO @ Wed, 22 Jul 2020 11:48:07: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 11:48:07: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:48:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 11:48:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:48:18: 5000000 INFO @ Wed, 22 Jul 2020 11:48:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:48:20: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:48:20: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:48:23: 6000000 INFO @ Wed, 22 Jul 2020 11:48:25: 1000000 INFO @ Wed, 22 Jul 2020 11:48:29: 7000000 INFO @ Wed, 22 Jul 2020 11:48:31: 2000000 INFO @ Wed, 22 Jul 2020 11:48:33: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 11:48:33: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 11:48:33: #1 total tags in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:48:33: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:48:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:48:33: #1 tags after filtering in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:48:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:48:33: #1 finished! INFO @ Wed, 22 Jul 2020 11:48:33: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:48:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:48:34: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 11:48:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:48:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Jul 2020 11:48:37: 3000000 INFO @ Wed, 22 Jul 2020 11:48:42: 4000000 INFO @ Wed, 22 Jul 2020 11:48:48: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:48:53: 6000000 INFO @ Wed, 22 Jul 2020 11:48:58: 7000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 11:49:02: #1 tag size is determined as 50 bps INFO @ Wed, 22 Jul 2020 11:49:02: #1 tag size = 50 INFO @ Wed, 22 Jul 2020 11:49:02: #1 total tags in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:49:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:49:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:49:02: #1 tags after filtering in treatment: 7727264 INFO @ Wed, 22 Jul 2020 11:49:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:49:02: #1 finished! INFO @ Wed, 22 Jul 2020 11:49:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:49:03: #2 number of paired peaks: 0 WARNING @ Wed, 22 Jul 2020 11:49:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:49:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX1967616/SRX1967616.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling