Job ID = 2009859 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 49,275,093 reads read : 49,275,093 reads written : 49,275,093 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR585739.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:07 49275093 reads; of these: 49275093 (100.00%) were unpaired; of these: 15449869 (31.35%) aligned 0 times 27390497 (55.59%) aligned exactly 1 time 6434727 (13.06%) aligned >1 times 68.65% overall alignment rate Time searching: 00:06:07 Overall time: 00:06:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 23761195 / 33825224 = 0.7025 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:26:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:26:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:26:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:26:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:26:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:26:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:26:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:26:29: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:26:29: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:26:37: 1000000 INFO @ Fri, 05 Jul 2019 20:26:38: 1000000 INFO @ Fri, 05 Jul 2019 20:26:38: 1000000 INFO @ Fri, 05 Jul 2019 20:26:46: 2000000 INFO @ Fri, 05 Jul 2019 20:26:47: 2000000 INFO @ Fri, 05 Jul 2019 20:26:47: 2000000 INFO @ Fri, 05 Jul 2019 20:26:55: 3000000 INFO @ Fri, 05 Jul 2019 20:26:56: 3000000 INFO @ Fri, 05 Jul 2019 20:26:56: 3000000 INFO @ Fri, 05 Jul 2019 20:27:04: 4000000 INFO @ Fri, 05 Jul 2019 20:27:04: 4000000 INFO @ Fri, 05 Jul 2019 20:27:05: 4000000 INFO @ Fri, 05 Jul 2019 20:27:13: 5000000 INFO @ Fri, 05 Jul 2019 20:27:13: 5000000 INFO @ Fri, 05 Jul 2019 20:27:14: 5000000 INFO @ Fri, 05 Jul 2019 20:27:21: 6000000 INFO @ Fri, 05 Jul 2019 20:27:22: 6000000 INFO @ Fri, 05 Jul 2019 20:27:23: 6000000 INFO @ Fri, 05 Jul 2019 20:27:30: 7000000 INFO @ Fri, 05 Jul 2019 20:27:31: 7000000 INFO @ Fri, 05 Jul 2019 20:27:31: 7000000 INFO @ Fri, 05 Jul 2019 20:27:38: 8000000 INFO @ Fri, 05 Jul 2019 20:27:40: 8000000 INFO @ Fri, 05 Jul 2019 20:27:40: 8000000 INFO @ Fri, 05 Jul 2019 20:27:46: 9000000 INFO @ Fri, 05 Jul 2019 20:27:49: 9000000 INFO @ Fri, 05 Jul 2019 20:27:49: 9000000 INFO @ Fri, 05 Jul 2019 20:27:54: 10000000 INFO @ Fri, 05 Jul 2019 20:27:55: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:27:55: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:27:55: #1 total tags in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:55: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:27:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:27:55: #1 tags after filtering in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:55: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:27:55: #1 finished! INFO @ Fri, 05 Jul 2019 20:27:55: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:27:55: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:27:56: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:27:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:27:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:27:58: 10000000 INFO @ Fri, 05 Jul 2019 20:27:58: 10000000 INFO @ Fri, 05 Jul 2019 20:27:58: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:27:58: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:27:58: #1 total tags in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:27:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:27:58: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:27:58: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:27:58: #1 total tags in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:27:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:27:58: #1 tags after filtering in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:27:58: #1 finished! INFO @ Fri, 05 Jul 2019 20:27:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:27:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:27:59: #1 tags after filtering in treatment: 10064029 INFO @ Fri, 05 Jul 2019 20:27:59: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:27:59: #1 finished! INFO @ Fri, 05 Jul 2019 20:27:59: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:27:59: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:27:59: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:27:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:27:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:27:59: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:27:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:27:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193518/SRX193518.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。