Job ID = 2009856 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 59,434,734 reads read : 59,434,734 reads written : 59,434,734 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR585736.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:24 59434734 reads; of these: 59434734 (100.00%) were unpaired; of these: 14955498 (25.16%) aligned 0 times 40877537 (68.78%) aligned exactly 1 time 3601699 (6.06%) aligned >1 times 74.84% overall alignment rate Time searching: 00:07:24 Overall time: 00:07:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 30262002 / 44479236 = 0.6804 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:30:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:30:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:30:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:30:41: 1000000 INFO @ Fri, 05 Jul 2019 20:30:41: 1000000 INFO @ Fri, 05 Jul 2019 20:30:42: 1000000 INFO @ Fri, 05 Jul 2019 20:30:47: 2000000 INFO @ Fri, 05 Jul 2019 20:30:48: 2000000 INFO @ Fri, 05 Jul 2019 20:30:49: 2000000 INFO @ Fri, 05 Jul 2019 20:30:53: 3000000 INFO @ Fri, 05 Jul 2019 20:30:54: 3000000 INFO @ Fri, 05 Jul 2019 20:30:55: 3000000 INFO @ Fri, 05 Jul 2019 20:30:59: 4000000 INFO @ Fri, 05 Jul 2019 20:31:01: 4000000 INFO @ Fri, 05 Jul 2019 20:31:01: 4000000 INFO @ Fri, 05 Jul 2019 20:31:05: 5000000 INFO @ Fri, 05 Jul 2019 20:31:07: 5000000 INFO @ Fri, 05 Jul 2019 20:31:07: 5000000 INFO @ Fri, 05 Jul 2019 20:31:12: 6000000 INFO @ Fri, 05 Jul 2019 20:31:13: 6000000 INFO @ Fri, 05 Jul 2019 20:31:14: 6000000 INFO @ Fri, 05 Jul 2019 20:31:18: 7000000 INFO @ Fri, 05 Jul 2019 20:31:19: 7000000 INFO @ Fri, 05 Jul 2019 20:31:20: 7000000 INFO @ Fri, 05 Jul 2019 20:31:24: 8000000 INFO @ Fri, 05 Jul 2019 20:31:26: 8000000 INFO @ Fri, 05 Jul 2019 20:31:27: 8000000 INFO @ Fri, 05 Jul 2019 20:31:30: 9000000 INFO @ Fri, 05 Jul 2019 20:31:32: 9000000 INFO @ Fri, 05 Jul 2019 20:31:33: 9000000 INFO @ Fri, 05 Jul 2019 20:31:36: 10000000 INFO @ Fri, 05 Jul 2019 20:31:38: 10000000 INFO @ Fri, 05 Jul 2019 20:31:39: 10000000 INFO @ Fri, 05 Jul 2019 20:31:42: 11000000 INFO @ Fri, 05 Jul 2019 20:31:44: 11000000 INFO @ Fri, 05 Jul 2019 20:31:46: 11000000 INFO @ Fri, 05 Jul 2019 20:31:48: 12000000 INFO @ Fri, 05 Jul 2019 20:31:50: 12000000 INFO @ Fri, 05 Jul 2019 20:31:52: 12000000 INFO @ Fri, 05 Jul 2019 20:31:54: 13000000 INFO @ Fri, 05 Jul 2019 20:31:56: 13000000 INFO @ Fri, 05 Jul 2019 20:31:58: 13000000 INFO @ Fri, 05 Jul 2019 20:32:00: 14000000 INFO @ Fri, 05 Jul 2019 20:32:01: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:32:01: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:32:01: #1 total tags in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:32:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:32:01: #1 tags after filtering in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:32:01: #1 finished! INFO @ Fri, 05 Jul 2019 20:32:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:32:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:32:02: 14000000 INFO @ Fri, 05 Jul 2019 20:32:02: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:32:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:32:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:32:03: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:32:03: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:32:03: #1 total tags in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:32:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:32:03: #1 tags after filtering in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:32:03: #1 finished! INFO @ Fri, 05 Jul 2019 20:32:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:32:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:32:04: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:32:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:32:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 20:32:05: 14000000 pass1 - making usageList (0 chroms): 19 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:32:06: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 20:32:06: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 20:32:06: #1 total tags in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:06: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:32:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:32:06: #1 tags after filtering in treatment: 14217234 INFO @ Fri, 05 Jul 2019 20:32:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 20:32:06: #1 finished! INFO @ Fri, 05 Jul 2019 20:32:06: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:32:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:32:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:32:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:32:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193515/SRX193515.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。