Job ID = 2009855 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T11:08:28 fasterq-dump.2.9.6 fatal: SIGNAL - Segmentation fault spots read : 54,415,304 reads read : 54,415,304 reads written : 54,415,304 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR585735.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:16 54415304 reads; of these: 54415304 (100.00%) were unpaired; of these: 10804401 (19.86%) aligned 0 times 38333690 (70.45%) aligned exactly 1 time 5277213 (9.70%) aligned >1 times 80.14% overall alignment rate Time searching: 00:08:16 Overall time: 00:08:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 29399514 / 43610903 = 0.6741 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:05:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:05:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:05:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:05:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:05:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:05:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:05:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:05:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:05:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:05:30: 1000000 INFO @ Fri, 05 Jul 2019 21:05:30: 1000000 INFO @ Fri, 05 Jul 2019 21:05:30: 1000000 INFO @ Fri, 05 Jul 2019 21:05:38: 2000000 INFO @ Fri, 05 Jul 2019 21:05:39: 2000000 INFO @ Fri, 05 Jul 2019 21:05:39: 2000000 INFO @ Fri, 05 Jul 2019 21:05:45: 3000000 INFO @ Fri, 05 Jul 2019 21:05:47: 3000000 INFO @ Fri, 05 Jul 2019 21:05:48: 3000000 INFO @ Fri, 05 Jul 2019 21:05:52: 4000000 INFO @ Fri, 05 Jul 2019 21:05:55: 4000000 INFO @ Fri, 05 Jul 2019 21:05:56: 4000000 INFO @ Fri, 05 Jul 2019 21:05:59: 5000000 INFO @ Fri, 05 Jul 2019 21:06:03: 5000000 INFO @ Fri, 05 Jul 2019 21:06:04: 5000000 INFO @ Fri, 05 Jul 2019 21:06:06: 6000000 INFO @ Fri, 05 Jul 2019 21:06:11: 6000000 INFO @ Fri, 05 Jul 2019 21:06:12: 6000000 INFO @ Fri, 05 Jul 2019 21:06:13: 7000000 INFO @ Fri, 05 Jul 2019 21:06:20: 7000000 INFO @ Fri, 05 Jul 2019 21:06:20: 7000000 INFO @ Fri, 05 Jul 2019 21:06:20: 8000000 INFO @ Fri, 05 Jul 2019 21:06:27: 9000000 INFO @ Fri, 05 Jul 2019 21:06:28: 8000000 INFO @ Fri, 05 Jul 2019 21:06:28: 8000000 INFO @ Fri, 05 Jul 2019 21:06:34: 10000000 INFO @ Fri, 05 Jul 2019 21:06:36: 9000000 INFO @ Fri, 05 Jul 2019 21:06:36: 9000000 INFO @ Fri, 05 Jul 2019 21:06:41: 11000000 INFO @ Fri, 05 Jul 2019 21:06:43: 10000000 INFO @ Fri, 05 Jul 2019 21:06:44: 10000000 INFO @ Fri, 05 Jul 2019 21:06:48: 12000000 INFO @ Fri, 05 Jul 2019 21:06:51: 11000000 INFO @ Fri, 05 Jul 2019 21:06:52: 11000000 INFO @ Fri, 05 Jul 2019 21:06:55: 13000000 INFO @ Fri, 05 Jul 2019 21:06:59: 12000000 INFO @ Fri, 05 Jul 2019 21:07:00: 12000000 INFO @ Fri, 05 Jul 2019 21:07:02: 14000000 INFO @ Fri, 05 Jul 2019 21:07:03: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 21:07:03: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 21:07:03: #1 total tags in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:07:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:07:04: #1 tags after filtering in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:04: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:07:04: #1 finished! INFO @ Fri, 05 Jul 2019 21:07:04: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:07:04: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:07:05: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:07:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:07:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:07:07: 13000000 INFO @ Fri, 05 Jul 2019 21:07:08: 13000000 INFO @ Fri, 05 Jul 2019 21:07:15: 14000000 INFO @ Fri, 05 Jul 2019 21:07:16: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 21:07:16: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 21:07:16: #1 total tags in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:07:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:07:17: 14000000 INFO @ Fri, 05 Jul 2019 21:07:17: #1 tags after filtering in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:07:17: #1 finished! INFO @ Fri, 05 Jul 2019 21:07:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:07:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:07:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:07:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:07:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:07:18: #1 tag size is determined as 36 bps INFO @ Fri, 05 Jul 2019 21:07:18: #1 tag size = 36 INFO @ Fri, 05 Jul 2019 21:07:18: #1 total tags in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:07:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:07:19: #1 tags after filtering in treatment: 14211389 INFO @ Fri, 05 Jul 2019 21:07:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:07:19: #1 finished! INFO @ Fri, 05 Jul 2019 21:07:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:07:20: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:07:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:07:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193514/SRX193514.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。