Job ID = 2009848 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 16,000,000 reads read : 32,000,000 reads written : 32,000,000 spots read : 838,570 reads read : 1,677,140 reads written : 1,677,140 rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR583971.sra.cache’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR583972.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:39 16838570 reads; of these: 16838570 (100.00%) were paired; of these: 5937235 (35.26%) aligned concordantly 0 times 9884598 (58.70%) aligned concordantly exactly 1 time 1016737 (6.04%) aligned concordantly >1 times ---- 5937235 pairs aligned concordantly 0 times; of these: 2323766 (39.14%) aligned discordantly 1 time ---- 3613469 pairs aligned 0 times concordantly or discordantly; of these: 7226938 mates make up the pairs; of these: 5265766 (72.86%) aligned 0 times 1443770 (19.98%) aligned exactly 1 time 517402 (7.16%) aligned >1 times 84.36% overall alignment rate Time searching: 00:16:39 Overall time: 00:16:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 2664814 / 12948385 = 0.2058 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 20:43:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:43:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:43:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:43:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:43:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:43:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:43:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 20:43:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 20:43:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 20:43:39: 1000000 INFO @ Fri, 05 Jul 2019 20:43:40: 1000000 INFO @ Fri, 05 Jul 2019 20:43:45: 1000000 INFO @ Fri, 05 Jul 2019 20:43:46: 2000000 INFO @ Fri, 05 Jul 2019 20:43:49: 2000000 INFO @ Fri, 05 Jul 2019 20:43:51: 2000000 INFO @ Fri, 05 Jul 2019 20:43:52: 3000000 INFO @ Fri, 05 Jul 2019 20:43:55: 3000000 INFO @ Fri, 05 Jul 2019 20:43:57: 3000000 INFO @ Fri, 05 Jul 2019 20:43:58: 4000000 INFO @ Fri, 05 Jul 2019 20:44:01: 4000000 INFO @ Fri, 05 Jul 2019 20:44:04: 4000000 INFO @ Fri, 05 Jul 2019 20:44:05: 5000000 INFO @ Fri, 05 Jul 2019 20:44:07: 5000000 INFO @ Fri, 05 Jul 2019 20:44:10: 5000000 INFO @ Fri, 05 Jul 2019 20:44:11: 6000000 INFO @ Fri, 05 Jul 2019 20:44:13: 6000000 INFO @ Fri, 05 Jul 2019 20:44:16: 6000000 INFO @ Fri, 05 Jul 2019 20:44:17: 7000000 INFO @ Fri, 05 Jul 2019 20:44:18: 7000000 INFO @ Fri, 05 Jul 2019 20:44:24: 7000000 INFO @ Fri, 05 Jul 2019 20:44:24: 8000000 INFO @ Fri, 05 Jul 2019 20:44:24: 8000000 INFO @ Fri, 05 Jul 2019 20:44:30: 8000000 INFO @ Fri, 05 Jul 2019 20:44:30: 9000000 INFO @ Fri, 05 Jul 2019 20:44:30: 9000000 INFO @ Fri, 05 Jul 2019 20:44:36: 9000000 INFO @ Fri, 05 Jul 2019 20:44:37: 10000000 INFO @ Fri, 05 Jul 2019 20:44:37: 10000000 INFO @ Fri, 05 Jul 2019 20:44:43: 11000000 INFO @ Fri, 05 Jul 2019 20:44:43: 10000000 INFO @ Fri, 05 Jul 2019 20:44:43: 11000000 INFO @ Fri, 05 Jul 2019 20:44:48: 12000000 INFO @ Fri, 05 Jul 2019 20:44:49: 11000000 INFO @ Fri, 05 Jul 2019 20:44:50: 12000000 INFO @ Fri, 05 Jul 2019 20:44:54: 13000000 INFO @ Fri, 05 Jul 2019 20:44:55: 12000000 INFO @ Fri, 05 Jul 2019 20:44:56: 13000000 INFO @ Fri, 05 Jul 2019 20:45:00: 14000000 INFO @ Fri, 05 Jul 2019 20:45:02: 13000000 INFO @ Fri, 05 Jul 2019 20:45:03: 14000000 INFO @ Fri, 05 Jul 2019 20:45:06: 15000000 INFO @ Fri, 05 Jul 2019 20:45:08: 14000000 INFO @ Fri, 05 Jul 2019 20:45:09: 15000000 INFO @ Fri, 05 Jul 2019 20:45:12: 16000000 INFO @ Fri, 05 Jul 2019 20:45:15: 15000000 INFO @ Fri, 05 Jul 2019 20:45:15: 16000000 INFO @ Fri, 05 Jul 2019 20:45:17: 17000000 INFO @ Fri, 05 Jul 2019 20:45:21: 16000000 INFO @ Fri, 05 Jul 2019 20:45:22: 17000000 INFO @ Fri, 05 Jul 2019 20:45:23: 18000000 INFO @ Fri, 05 Jul 2019 20:45:27: 17000000 INFO @ Fri, 05 Jul 2019 20:45:28: 18000000 INFO @ Fri, 05 Jul 2019 20:45:30: 19000000 INFO @ Fri, 05 Jul 2019 20:45:34: 18000000 INFO @ Fri, 05 Jul 2019 20:45:35: 19000000 INFO @ Fri, 05 Jul 2019 20:45:35: 20000000 INFO @ Fri, 05 Jul 2019 20:45:41: 19000000 INFO @ Fri, 05 Jul 2019 20:45:41: 20000000 INFO @ Fri, 05 Jul 2019 20:45:41: 21000000 INFO @ Fri, 05 Jul 2019 20:45:47: 21000000 INFO @ Fri, 05 Jul 2019 20:45:47: 22000000 INFO @ Fri, 05 Jul 2019 20:45:47: 20000000 INFO @ Fri, 05 Jul 2019 20:45:53: 23000000 INFO @ Fri, 05 Jul 2019 20:45:53: 22000000 INFO @ Fri, 05 Jul 2019 20:45:54: #1 tag size is determined as 101 bps INFO @ Fri, 05 Jul 2019 20:45:54: #1 tag size = 101 INFO @ Fri, 05 Jul 2019 20:45:54: #1 total tags in treatment: 8628399 INFO @ Fri, 05 Jul 2019 20:45:54: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:45:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:45:54: #1 tags after filtering in treatment: 4139423 INFO @ Fri, 05 Jul 2019 20:45:54: #1 Redundant rate of treatment: 0.52 INFO @ Fri, 05 Jul 2019 20:45:54: #1 finished! INFO @ Fri, 05 Jul 2019 20:45:54: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:45:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:45:54: 21000000 INFO @ Fri, 05 Jul 2019 20:45:54: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:45:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:45:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:45:59: 23000000 INFO @ Fri, 05 Jul 2019 20:46:00: 22000000 INFO @ Fri, 05 Jul 2019 20:46:00: #1 tag size is determined as 101 bps INFO @ Fri, 05 Jul 2019 20:46:00: #1 tag size = 101 INFO @ Fri, 05 Jul 2019 20:46:00: #1 total tags in treatment: 8628399 INFO @ Fri, 05 Jul 2019 20:46:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:46:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:46:01: #1 tags after filtering in treatment: 4139423 INFO @ Fri, 05 Jul 2019 20:46:01: #1 Redundant rate of treatment: 0.52 INFO @ Fri, 05 Jul 2019 20:46:01: #1 finished! INFO @ Fri, 05 Jul 2019 20:46:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:46:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:46:01: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:46:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:46:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 20:46:05: 23000000 INFO @ Fri, 05 Jul 2019 20:46:06: #1 tag size is determined as 101 bps INFO @ Fri, 05 Jul 2019 20:46:06: #1 tag size = 101 INFO @ Fri, 05 Jul 2019 20:46:06: #1 total tags in treatment: 8628399 INFO @ Fri, 05 Jul 2019 20:46:06: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 20:46:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 20:46:06: #1 tags after filtering in treatment: 4139423 INFO @ Fri, 05 Jul 2019 20:46:06: #1 Redundant rate of treatment: 0.52 INFO @ Fri, 05 Jul 2019 20:46:06: #1 finished! INFO @ Fri, 05 Jul 2019 20:46:06: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 20:46:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 20:46:06: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 20:46:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 20:46:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX193165/SRX193165.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。